Anti-GABA-A-R, Beta3 Antibody (N87/25)
Our Anti-GABA(A)R, Beta3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N87/25. It detects human and mouse GABA(A)R, Beta3, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
![Immunoblot against membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N87/25 (left) or N52A/42 (right) TC supe.](http://www.antibodiesinc.com/cdn/shop/files/73-149-n87-25-wb-1_{width}x.jpg?v=1703224571)
Adult rat brain membrane immunoblot.
![Immunoblot against membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N87/25 (left) or N52A/42 (right) TC supe.](http://www.antibodiesinc.com/cdn/shop/files/73-149-n87-25-wb-1_1024x.jpg?v=1703224571)
Product Specific References for Applications and Species
- Immunocytochemistry: Human | Mouse| Rat
- Immunohistochemistry: Mouse| Rat
- Immunoprecipitation: Rat
- Western Blot: Human | Mouse | Rat
- Additional Publications: Unspecified
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 23236276 | 1:250 | Fuks, J.M., et al. 2012. GABAergic signaling is linked to a hypermigratory phenotype in dendritic cells infected by Toxoplasma gondii.. PLoS Pathogens, e1003051. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 36997609 | 1:300 | Giansante, G., et al. 2023. Neuronal Network Activity and Connectivity are Impaired in a Conditional Knockout Mouse Model with PCDH19 Mosaic Expression. Molecular Psychiatry, . |
| 34936876 | 1:1000 | Garcia, J.D., et al. 2021. Stepwise disassembly of GABAergic synapses during pathogenic excitotoxicity. Cell Reports, 110142. |
| 31835170 | 1:500 | Niwa, F., et al. 2019. cAMP-EPAC-Dependent Regulation of Gephyrin Phosphorylation and GABAAR Trapping at Inhibitory Synapses. iScience, 453-465. |
| 31665629 | 1:200 | Moretto, E., et al. 2019. TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering. Cell Reports, 1130-1146.e8. |
| 31315046 | not listed | Nathanson, A.J., et al. 2019. Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures. Cell Reports, 670-681. |
| 30667360 | 1:5 (supe) | Andrews, N.P., et al. 2019. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife, e43322. |
| 23672680 | 1:250 | Alicea, B., et al. 2013. Defining the diversity of phenotypic respecification using multiple cell lines and reprogramming regimens.. Stem Cells and Development, 2641-2654. |
| 21735059 | 1:500 | Yanovsky, Y., et al. 2012. GABAA receptors involved in sleep and anaesthesia: β1- versus β3-containing assemblies.. Pflügers Archiv: European Journal of Physiology, 187-199. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 34047007 | 1:500 | Yang, X., et al. 2021. Differential regulation of glycinergic and GABAergic nanocolumns at mixed inhibitory synapses. EMBO Reports, e52154. |
| 31665629 | 1:200 | Moretto, E., et al. 2019. TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering. Cell Reports, 1130-1146.e8. |
| 28262662 | 1:300 | Zapata, J., et al. 2017. Epilepsy and intellectual disability linked protein Shrm4 interaction with GABABRs shapes inhibitory neurotransmission. Nature Communications, 14536. |
| 22927941 | 1:500 | , et al. 2012. Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes.. PLoS One, e42959. |
| 20417281 | 1:100 (supe) | Smith, K.R., et al. 2010. Identification and characterisation of a Maf1/Macoco protein complex that interacts with GABAA receptors in neurons.. Molecular and Cellular Neuroscience, 330-341. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 26179122 | 1:1000 | He, Q., et al. 2015. Interneuron- and GABA(A) receptor-specific inhibitory synaptic plasticity in cerebellar Purkinje cells.. Nature Communications, 7364. |
| 22351636 | 1:1000 | Sumegi, M., et al. 2012. Virus-mediated swapping of zolpidem-insensitive with zolpidem-sensitive GABA(A) receptors in cortical pyramidal cells.. The Journal of Physiology, 1517-1534. |
| 21482824 | 1:500 | Jo, Y.H., et al. 2011. Cross-talk between P2X4 and gamma-aminobutyric acid, type A receptors determines synaptic efficacy at a central synapse.. The Journal of Biological Chemistry, 19993-20004. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 27537197 | 1:1000 | Kerti-Szigeti, K., et al. 2016. Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells. Elife, e18426. |
| 24477962 | 1:5 (supe) | King, A.N., et al. 2014. A unique ion channel clustering domain on the axon initial segment of mammalian neurons. . Journal of Comparative Neurology, 2594-2608. |
| 22539847 | 1:2000 | , et al. 2012. Setting the time course of inhibitory synaptic currents by mixing multiple GABA(A) receptor α subunit isoforms.. Journal of Neuroscience, 5853-5867. |
| 22098631 | 1:800 | Kerti, K., et al. 2012. Unique somato-dendritic distribution pattern of Kv4.2 channels on hippocampal CA1 pyramidal cells.. European Journal of Neuroscience, 66-75. |
| 19815531 | not listed | Arancibia-Cárcamo, I.L., et al. 2009. Ubiquitin-dependent lysosomal targeting of GABA(A) receptors regulates neuronal inhibition.. PNAS: USA, 17552-17557. |
| Immunoprecipitation: Rat | ||
| PMID | Dilution | Publication |
| 24089494 | 2ug | Pribiag, H., et al. 2014. TNF-α downregulates inhibitory neurotransmission through protein phosphatase 1-dependent trafficking of GABA(A) receptors.. Journal of Neuroscience, 15879-15893. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 22303015 | 4ug/ml | Gurba, K.N., et al. 2012. GABRB3 mutation, G32R, associated with childhood absence epilepsy alters α1β3γ2L γ-aminobutyric acid type A (GABAA) receptor expression and channel gating.. The Journal of Biological Chemistry, 12083. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 34936876 | 1:5000 | Garcia, J.D., et al. 2021. Stepwise disassembly of GABAergic synapses during pathogenic excitotoxicity. Cell Reports, 110142. |
| 33147470 | not listed | Rudolph, S., et al. 2020. ArticleCerebellum-Specific Deletion of the GABAAReceptordSubunit Leads to Sex-Specific Disruptionof Behavior. Cell Reports, 108338. |
| 30791981 | not listed | Fu, Y.L., et al. 2019. LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors. Biochemical and Biophysical Research Communications, 356-362. |
| 28154140 | 1 : 500 | Sterky, F.H., et al. 2017. Carbonic anhydrase-related protein CA10 is an evolutionarily conserved pan-neurexin ligand.. PNAS: USA, E1253-E1262. |
| 27044742 | 1:1000 | Nakamura, Y., et al. 2016. Proteomic Characterization of Inhibitory Synapses Using a Novel pHluorin-tagged γ-Aminobutyric Acid Receptor, Type A (GABAA), α2 Subunit Knock-in Mouse. Journal of Biological Chemistry, 12394-12407. |
| 24153177 | not listed | Han, K., et al. 2014. SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties.. Nature, 72-77. |
| 23744069 | 1:100 | Zhou, C., et al. 2013. Altered cortical GABAA receptor composition, physiology, and endocytosis in a mouse model of a human genetic absence epilepsy syndrome.. The Journal of Biological Chemistry, 21458-21472. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 32579917 | 1:1000 | Rajgor, D., et al. 2020. Local miRNA-dependent translational control of GABAAR synthesis during inhibitory long-term potentiation. Cell reports, 31(12), p.107785.. Cell reports, 107785. |
| 25310985 | not listed | Piguel, N.H., et al. 2014. Scribble1/AP2 complex coordinates NMDA receptor endocytic recycling. Cell Reports, 712-727. |
| 24089494 | 1:500 | Pribiag, H., et al. 2014. TNF-α downregulates inhibitory neurotransmission through protein phosphatase 1-dependent trafficking of GABA(A) receptors.. Journal of Neuroscience, 15879-15893. |
| 20417281 | 1:10 (supe) | Smith, K.R., et al. 2010. Identification and characterisation of a Maf1/Macoco protein complex that interacts with GABAA receptors in neurons.. Molecular and Cellular Neuroscience, 330-341. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 26732589 | Mele, M., et al. 2017. Downregulation of GABAA Receptor Recycling Mediated by HAP1 Contributes to Neuronal Death in In Vitro Brain Ischemia.. Molecular Neurobiology, 45-57. | |
| 25510230 | Babateen, O., et al. 2015. Etomidate, propofol and diazepam potentiate GABA-evoked GABAA currents in a cell line derived from human glioblastoma.. European Journal of Pharmacology, 101-107. | |
| 25402556 | Marty, V.N., et al. 2015. Radiation-induced alterations in synaptic neurotransmission of dentate granule cells depend on the dose and species of charged particles.. Radiation Research, 653-665. | |
| 24513087 | Mele, M., et al. 2014. GABA(A) receptor dephosphorylation followed by internalization is coupled to neuronal death in in vitro ischemia.. Neurobiology of Disease, 220-232. | |
| 20980909 | Fagerlund, M.J., et al. 2010. The human carotid body: expression of oxygen sensing and signaling genes of relevance for anesthesia.. Anesthesiology, 1270-1279. | |
| 20470805 | Adusei, D.C., et al. 2010. Early developmental alterations in GABAergic protein expression in fragile X knockout mice.. Neuropharmacology, 167-171. | |