Anti-Gamma-Protocadherin-A3 Antibody (N144/17)

Name: Anti-Gamma-Protocadherin-A3 Antibody
Brand: NeuroMab
SKU: 75-177
Price: 319.0 USD
Availability: InStock

2 Citations

Our Anti-Gamma-protocadherin-A3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N144/17. It is KO validated, detects mouse Gamma-protocadherin-A3, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Gamma- protocadherin-A3 and -A4 and untagged Kv2.1 plasmids and probed with N144/17 (left) and N159/5 (right) TC supe.

Species Reactivity

Mouse

Applications

ICC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-177

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Gamma-protocadherin-A3

Target Description

Protocadherin gamma A3 is encoded by the gene Pcdhga3. Gamma-protocadherins belong to a superfamily of homophilic cell-adhesion proteins. These cell adhesion proteins are thought to be involved in establishing and function of cell-cell connections in brain. Gamma-Protocadherin-A3 is a type I transmembrane receptor and is a calcium-dependent cell-adhesion protein. Gamma-Protocadherin-A3 plays a role in the establishment and maintenance of specific neuronal connections in the brain. Gamma-Protocadherin-A3 is expressed in the central nervous system where it localizes to synapses. Diseases associated with this gene include Pineal Gland Cancer.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N144/17

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

Pcdhga3

Molecular Weight

100 kDa

Antigen

Fusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma-protocadherin-A3 (accession number Q91XY5) produced recombinantly in E. Coli

Species Reactivity

Mouse

Antibody Registry ID

AB_10671815

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.105

Conjugate

Unconjugated

Specificity

No cross-reactivity against other Gamma-protocadherin-A, -B or -C proteins

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Protocadherin gamma A3 (Protocadherin gamma subfamily A, 3)

UniProt Details

UniProt (Human): Q9Y5H0
UniProt (Immunogen Species): Q91XY5

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
22884324	not listed	Chen, W.V., et al. 2012. Functional significance of isoform diversification in the protocadherin gamma gene cluster.. Neuron, 402-409.
22092001	not listed	Lobas, M.A., et al. 2012. Molecular heterogeneity in the choroid plexus epithelium: the 22-member γ-protocadherin family is differentially expressed, apically localized, and implicated in CSF regulation.. Journal of Neurochemistry, 913-927.

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Anti-Gamma-Protocadherin-A3 Antibody (N144/17)

SKU: 75-177

Product Details

Product Specific References for Applications and Species

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