Anti-GIT2 Antibody (N83/48)

Name: Anti-GIT2 Antibody
Brand: NeuroMab
SKU: 75-126
Price: 319.0 USD
Availability: InStock

Our Anti-GIT2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N83/48. It detects human, mouse, and rat GIT2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Transfected cell immunoblot: extracts of rat brain membranes (RBM) and COS cells transiently transfected with Flag-tagged GIT1 and GIT2 and untagged Kv2.1 plasmids and probed with N83/48 (left) and K89/41 (right) TC supe and with anti-Flag antibody (middle).

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-126

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

GIT2

Target Description

G-protein coupled receptor kinase interacting ArfGAP 2 is encoded by the gene GIT2. GIT2 is a member of the GIT protein family. GIT2 has a be shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. GIT2 is expressed in 207 organs with the highest expression level in the right hemisphere of cerebellum. Diseases associated with GIT2 include Gastric Antral Vascular Ectasia and Systemic Scleroderma.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N83/48

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

GIT2 KIAA0148

Molecular Weight

85 kDa

Antigen

Fusion protein amino acids 450-759 (C-terminus) of human GIT2 (accession number Q14161) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2110013

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.53

Conjugate

Unconjugated

Specificity

No cross-reactivity against GIT1

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

ARF GTPase-activating protein GIT2 (ARF GAP GIT2) (Cool-interacting tyrosine-phosphorylated protein 2) (CAT-2) (CAT2) (G protein-coupled receptor kinase-interactor 2) (GRK-interacting protein 2)

UniProt Details

UniProt (Human): Q14161
UniProt (Immunogen Species): Q14161

Product Specific References for Applications and Species

Western Blot: Human | Rat

Western Blot: Human
PMID	Dilution	Publication
35318302	1:50	Zhang, S., et al. 2022. GIT1 protects against breast cancer growth through negative regulation of Notch. Nature Communications, 1537.
35312684	1:50	Schmerl, B., et al. 2022. The synaptic scaffold protein MPP2 interacts with GABAA receptors at the periphery of the postsynaptic density of glutamatergic synapses. PLoS biology, e3001503.
23784547	not listed	Aslan, J.E., et al. 2013. The PAK system links Rho GTPase signaling to thrombin-mediated platelet activation.. American Journal of Physiology-Cell Physiology, C519-528.
22606307	not listed	Martin, B., et al. 2012. VENNTURE--a novel Venn diagram investigational tool for multiple pharmacological dataset analysis.. PLoS One, e36911.

Western Blot: Rat
PMID	Dilution	Publication
22606319	not listed	Chadwick, W., et al. 2012. GIT2 acts as a potential keystone protein in functional hypothalamic networks associated with age-related phenotypic changes in rats.. PLoS One, e36975.
21179406	not listed	Chadwick, W., et al. 2010. Minimal peroxide exposure of neuronal cells induces multifaceted adaptive responses.. PLoS One, e14352.

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Anti-GIT2 Antibody (N83/48)

SKU: 75-126

Product Details

Product Specific References for Applications and Species

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