Anti-Kv1.5 K+ Channel Antibody (K7/45)

29 Citations

Our Anti-Kv1.5 K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K7/45. It detects human, mouse, and rat Kv1.5 K+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Immunoblot on adult rat brain membrane (RBM) and lysates from Kv1.5-transfected (COS+) and untransfected (COS-) COS cells.
Immunoblot on adult rat brain membrane (RBM) and lysates from Kv1.5-transfected (COS+) and untransfected (COS-) COS cells.
Roll over image to zoom in Click on image to zoom
Immunoblot on adult rat brain membrane (RBM) and lysates from Kv1.5-transfected (COS+) and untransfected (COS-) COS cells.
Immunofluorescence staining transiently Kv1.5-transfected COS cells.
Representative western blots for native IKur/Kv1.5, and H2S producing enzymes CBS, CTH and MPST at different pacing time periods (2–8 hr) in the absence of GYY4137 (a H2S donor) or with incubation with GYY4137 (horizontal line), compared to control unstimulated conditions (0 h) in HL-1 cells. β-actin was used as loading control in all western blots. Image from publication CC-BY-4.0. PMID:37336943
Representative proximity ligation images showing positive sites of interaction (red) between Kv1.5 (75-011, 1:50) and Kvβ1, and Kv1.5 and Kvβ2 in coronary arterial smooth muscle cells from sedentary and exercised mice. Singly labelled cells for Kv1.5 are shown as a control. DAPI nuclear stain is shown in blue. Image from publication CC-BY-4.0. PMID:38978788

Human, Mouse, Rat
ICC, IHC, IP, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet

SKU: 75-011

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

Kv1.5 K+ channel
Potassium voltage-gated channel subfamily A member 5 or Kv1.5 is encoded by the gene KCNA5. Kv1.5 is a potassium channel subunit is a member of the potassium channel, voltage-gated, shaker-related subfamily. Potassium channels form homotetrameric and heterotetrameric channels in the membrane with various other related proteins, including KCNA2, KCNA4, KCNA5, KCNA6, KCNA7. Kv1.5 is expressed in heart, including the atria nd ventricles. Kv1.5 channels are also expressed in many other organs, such as pulmonary arteries, brain, skeletal muscle. Diseases associated with KCNA5 include Familial Atrial Fibrillation and insulinoma.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
K7/45
IgG1
ICC, IHC, IP, WB
Mouse
Kcna5
70 kDa
Fusion protein amino acids 542-602 (cytoplasmic C-terminus) of rat Kv1.5 (accession number P19024) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2131324
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4
WB: 1:500
IHC: 1:2000
ICC: 1:100
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Potassium voltage-gated channel subfamily A member 5 (RCK7) (RK4) (Voltage-gated potassium channel subunit Kv1.5)
P19024

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
389787881:50Raph, S, et al. 2024. Kv beta complex facilitates exercise-induced augmentation of myocardial perfusion and cardiac growth. Frontiers in Cardiovascular Medicine, 1411354.
354406321:50Dwenger, M.M., et al. 2022. Pyridine Nucleotide Redox Potential in Coronary Smooth Muscle Couples Myocardial Blood Flow to Cardiac Metabolism. Nature Communications, 2051.
31639389not listedKilfoil, P.J., et al. 2019. Metabolic regulation of Kv channels and cardiac repolarization by Kvβ2 subunits. Journal of molecular and cell cardiology, 93–106.
283428891:40Nystoriak, M.A., et al. 2017. Heteromeric complexes of aldo-keto reductase auxiliary KVβ subunits (AKR6A) regulate sarcolemmal localization of KV1. 5 in coronary arterial myocytes. . Chemico-biological interactions, 210-217.
212332141:1000Fulton, S., et al. 2011. Contribution of Kv1.2 voltage-gated potassium channel to D2 autoreceptor regulation of axonal dopamine overflow.. The Journal of Biological Chemistry, 9360-9372.
202996861:50Cidad, P., et al. 2010. Characterization of ion channels involved in the proliferative response of femoral artery smooth muscle cells.. Arterioscler Thromb Vasc Biol., 1203-1211.
Immunocytochemistry: Rat
PMID Dilution Publication
279586601:100Kidd, M.W., et al. 2017. Angiotensin II reduces the surface abundance of KV1. 5 channels in arterial myocytes to stimulate vasoconstriction. . The Journal of Physiology, 1607-1618.
Immunohistochemistry: Mouse
PMID Dilution Publication
366409131:500Otuyemi, B., et al. 2023. Domain and Cell Type-specific Immunolocalisation of Voltage-gated Potassium Channels in the Mouse Striatum. Journal of Chemical Neuroanatomy, 102233.
287151171:5000Ohgomori, T., et al. 2017. Differential activation of neuronal and glial STAT 3 in the spinal cord of the SOD 1G93A mouse model of amyotrophic lateral sclerosis.. European Journal of Neuroscience, 2001-2014.
229878201:5000Yamada, J., et al. 2013. Novel objective classification of reactive microglia following hypoglossal axotomy using hierarchical cluster analysis.. Journal of Comparative Neurology, 1184-1201.
20299686not listedCidad, P., et al. 2010. Characterization of ion channels involved in the proliferative response of femoral artery smooth muscle cells.. Arterioscler Thromb Vasc Biol., 1203-1211.
190919621:1000Miller, M.N., et al. 2008. Region-specific spike-frequency acceleration in layer 5 pyramidal neurons mediated by Kv1 subunits.. Journal of Neuroscience, 13716-13726.
Immunohistochemistry: Rat
PMID Dilution Publication
23318870not listedOvsepian, S.V., et al. 2013. A defined heteromeric KV1 channel stabilizes the intrinsic pacemaking and regulates the output of deep cerebellar nuclear neurons to thalamic targets.. The Journal of Physiology, 1771-91.
Immunoprecipitation: Human
PMID Dilution Publication
280724642 μgXiao, G.S., et al. 2017. Genistein and tyrphostin AG556 decrease ultra‐rapidly activating delayed rectifier K+ current of human atria by inhibiting EGF receptor tyrosine kinase. . British Journal of Pharmacology, 454-467.
PLA: Mouse
PMID Dilution Publication
31639389not listedKilfoil, P.J., et al. 2019. Metabolic regulation of Kv channels and cardiac repolarization by Kvβ2 subunits.. Journal of Molecular and Cellular Cardiology, 93-106.
Water Blot: Mouse
PMID Dilution Publication
256708601:2 (supe)Nieves-Cintrón, M., et al. 2015. Selective down-regulation of KV2.1 function contributes to enhanced arterial tone during diabetes.. The Journal of Biological Chemistry, 7918-29.
Western Blot: Human
PMID Dilution Publication
20533281not listedStockwin, L.H., et al. 2010. Sodium dichloroacetate selectively targets cells with defects in the mitochondrial ETC.. International Journal of Cancer, 2510-2519.
18570888not listedNanduri, J., et al. 2008. Mitochondrial reactive oxygen species mediate hypoxic down-regulation of hERG channel protein.. Biochemical and Biophysical Research Communications, 309-314.
Western Blot: Mouse
PMID Dilution Publication
373369431:1000Al-Owais, MM, et al. 2023. Inhibition of the Voltage-gated Potassium Channel Kv1.5 by Hydrogen Sulfide Attenuates Remodeling Through S-nitrosylation-mediated Signaling. Communications Biology, 651.
36842733not listedMarkandeya, Y.S., et al. 2023. Caveolin-3 and Caveolae Regulate Ventricular Repolarization. Journal of Molecular and Cellular Cardiology, 38-49.
31028120not listedDong, X., et al. 2019. Dlg1 maintains dendritic cell function by securing voltage-gated K+ channel integrity. . The Journal of Immunology, 3187-3197.
262052951:500El Gebeily, G., et al. 2015. Estrogen regulation of the transient outward K(+) current involves estrogen receptor α in mouse heart.. Journal of Molecular and Cellular Cardiology, 85-94.
254470801:300Gillet, L., et al. 2015. Cardiac-specific ablation of synapse-associated protein SAP97 in mice decreases potassium currents but not sodium current. Heart Rhythm, 181-192.
247232631:100Dufour, M.A., et al. 2014. Somatodendritic ion channel expression in substantia nigra pars compacta dopaminergic neurons across postnatal development.. Journal of Neuroscience Research, 981-99.
24494598not listedSpeca, D.J., et al. 2014. Deletion of the Kv2.1 delayed rectifier potassium channel leads to neuronal and behavioral hyperexcitability.. Genes, Brain and Behavior, 394-408.
243756411:200Lopez-Izquierdo, A., et al. 2014. The absence of insulin signaling in the heart induces changes in potassium channel expression and ventricular repolarization.. American Journal of Physiology-Heart and Circulatory Physiology, H747-H754.
16943244not listedPott, C., et al. 2007. Mechanism of shortened action potential duration in Na+-Ca2+ exchanger knockout mice.. American Journal of Physiology-Cell Physiology, C968-73.
Western Blot: Rat
PMID Dilution Publication
279586601:1000Kidd, M.W., et al. 2017. Angiotensin II reduces the surface abundance of KV1. 5 channels in arterial myocytes to stimulate vasoconstriction. . The Journal of Physiology, 1607-1618.
19411284not listedTobin, A.A., et al. 2009. Loss of cerebrovascular Shaker-type K(+) channels: a shared vasodilator defect of genetic and renal hypertensive rats.. American Journal of Physiology-Heart and Circulatory Physiology, H293-H303.

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