Anti-Kvbeta1.2 K+ Channel Antibody (K47/42)

Name: Anti-Kvbeta1.2 K+ Channel Antibody
Brand: NeuroMab
SKU: 75-019
Price: 319.0 USD
Availability: InStock

4 Citations

Our Anti-Kvbeta1.2 K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K47/42. It is KO validated, detects human, mouse, and rat Kvbeta1.2 K+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

$Immunoblot of crude membrane fractions from adult heart ventricle (V) and brain (B) from wild-type (WT) and Kvß1 knockout (Kvβ1) mice probed with K47/42 (Kvβ1.2, top) and K17/70 (Kvβ2, bottom). Data courtesy of Jeanne Nerbonne, Washington University SOM. Reproduced with permission from 2005 Aimond et al. Circ Res.$

Adult rat hippocampus immunohistochemistry.

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with K37/89 (left) or N52A/42 (right) TC supe.$

Immunoblot of crude membrane fractions from adult heart ventricle (V) and brain (B) from wild-type (WT) and Kvß1 knockout (Kvβ1) mice probed with K47/42 (Kvβ1.2, top) and K17/70 (Kvβ2, bottom). Data courtesy of Jeanne Nerbonne, Washington University SOM. Reproduced with permission from 2005 Aimond et al. Circ Res.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-019

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Kvbeta1.2 K+ channel

Target Description

Voltage-gated potassium channel subunit beta-1 or Kvβ1.1 is encoded by the gene KCNAB1. Kvß1.1 is a member of the potassium channel, voltage-gated, shaker-related subfamily. Alternative splicing of KCNAB1 allows for three distinct proteins to be made, Kvß1.1, Kvß1.2, and Kvß1.3. Each of these has a unique N terminus. Kvβ1.1 is a cytoplasmic subunit that modulates the characteristics of the membrane spanning, channel-forming alpha-subunits. Beta subunits can promote the closure of channels or enhance channel activity depending on the channel members. Kvß1 is predominantly expressed in brain and found in hippocampus, cerebral cortex, caudate putamen, colliculus and cerebellum. Diseases associated with this gene include Episodic Ataxia, Type 1 and Familial Temporal Lobe Epilepsy, 3

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

K47/42

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

KCNAB1 KCNA1B

Molecular Weight

65 kDa

Antigen

Synthetic peptide amino acids 9-28 (ADIPSPKLGLPKSSESALKC, unique Nterminus) of human Kv1.2 (accession number NP_003462)

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10674579

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Voltage-gated potassium channel subunit beta-1 (EC 1.1.1.-) (K(+) channel subunit beta-1) (Kv-beta-1)

UniProt Details

UniProt (Human): Q14722
UniProt (Immunogen Species): Q14722-3

Product Specific References for Applications and Species

Immunocytochemistry: Mouse | Rat
Western Blot: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
24445605	not listed	Hund, T. J., et al. 2014. β(IV)-Spectrin regulates TREK-1 membrane targeting in the heart.. Cardiovascular Research, 166-75.

Immunocytochemistry: Rat
PMID	Dilution	Publication
23318870	not listed	Ovsepian, S. V., et al. 2013. A defined heteromeric KV1 channel stabilizes the intrinsic pacemaking and regulates the output of deep cerebellar nuclear neurons to thalamic targets.. The Journal of Physiology, 1771-91.

Western Blot: Mouse
PMID	Dilution	Publication
22426702	not listed	Tipparaju, S. M., et al. 2012. Interactions between the C-terminus of Kv1.5 and Kvβ regulate pyridine nucleotide-dependent changes in channel gating.. Pflügers Archiv: European Journal of Physiology, 799-818.
21507952	not listed	Cheng, L., et al. 2011. Cortactin is required for N-cadherin regulation of Kv1.5 channel function.. The Journal of Biological Chemistry, 20478-20489.

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Anti-Kvbeta1.2 K+ Channel Antibody (K47/42)

SKU: 75-019

Product Details

Product Specific References for Applications and Species

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