Anti-MAP3K12 Antibody (N377/20)

Name: Anti-MAP3K12 Antibody
Brand: NeuroMab
SKU: 75-355
Price: 374.0 USD
Availability: InStock

Our Anti-MAP3K12 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N377/20. It is KO validated, detects mouse and rat MAP3K12, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

$Immunoblot against extracts of COS cells transiently transfected with GFP-tagged MAP3K12, MAP3K13 or untagged Kv2.1 plasmid; adult rat brain low-speed pellet (RBLSP) or membrane (RBM) fractions; and membranes from MAP3K12 wild-type (WT) and gene- trap knockout (KO) mice probed with N377/20 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Aki Itoh and Takayuki Itoh (UC Davis).$

Adult rat brain immunohistochemistry.

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-355

Volume: 100 µL

Variant

Price:

$374.00

Quantity:

Product Details

Target

MAP3K12

Target Description

Mitogen-activated protein kinase kinase kinase 12, or MAP3K12 is a member of the serine/threonine protein kinase family. MAP3K12 is expressed many tissues including kidney, lung, heart, testis, gastrointestinal tract, stomach, liver and pancreas. In brain, MAP3K12 is mainly expressed in neurons at synaptic terminals. MAP3K12 is thought to initiate signalling cascades which effect the developing mammalian nervous system, including processes such as neuronal migration and axon growth.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N377/20

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Map3k12 Zpk

Molecular Weight

120 kDa

Antigen

Fusion protein amino acids 727-888 (C-terminus) of mouse MAP3K12 (accession number Q60700) produced recombinantly in E. Coli

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2315888

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with MAP3K13

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Mitogen-activated protein kinase kinase kinase 12 (EC 2.7.11.25) (Dual leucine zipper bearing kinase) (DLK) (Leucine-zipper protein kinase) (ZPK) (MAPK-upstream kinase) (MUK) (Mixed lineage kinase)

UniProt Details

UniProt (Human): Q12852
UniProt (Immunogen Species): Q60700

Product Specific References for Applications and Species

Western Blot: Mouse
Additional Publications: Unspecified

Western Blot: Mouse
PMID	Dilution	Publication
35101451	not listed	Lee B, et al. 2022. FK506-binding protein-like and FK506-binding protein 8 regulate dual leucine zipper kinase degradation and neuronal responses to axon injury. The Journal of biological chemistry, 101647.

Additional Publications: Unspecified
PMID	Publication
32659913	Goodwani S, et al. 2020. Dual Leucine Zipper Kinase Is Constitutively Active in the Adult Mouse Brain and Has Both Stress-Induced and Homeostatic Functions. International Journal of Molecular Sciences, 4849.

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Anti-MAP3K12 Antibody (N377/20)

SKU: 75-355

Product Details

Product Specific References for Applications and Species

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