Our Anti-MAP3K12 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N377/20. It is KO validated, detects mouse and rat MAP3K12, and is purified by Protein A chromatography. It is great for use in IHC, ICC.
Mitogen-activated protein kinase kinase kinase 12, or MAP3K12 is a member of the serine/threonine protein kinase family. MAP3K12 is expressed many tissues including kidney, lung, heart, testis, gastrointestinal tract, stomach, liver and pancreas. In brain, MAP3K12 is mainly expressed in neurons at synaptic terminals. MAP3K12 is thought to initiate signalling cascades which effect the developing mammalian nervous system, including processes such as neuronal migration and axon growth.
Purified by Protein A chromatography
0.5 mg/mL
Monoclonal
N377/20
IgG2a
ICC, IHC
Mouse
Map3k12 Zpk
120 kDa
Fusion protein amino acids 727-888 (C-terminus) of mouse MAP3K12 (accession number Q60700) produced recombinantly in E. Coli
Mouse, Rat
AB_2940193
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
PBS with 0.09% azide
FL550 Ex: 550 nm, Em: 575 nm
Does not cross-react with MAP3K13
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
