Anti-Mortalin/GRP75 Antibody (N52A/42)
Our Anti-Mortalin/GRP75 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N52A/42. It detects human, mouse, and rat Mortalin/GRP75, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
![Immunoblot against crude membranes prepared from whole rat (RBM) and mouse (MBM) brain and human cerebral cortex [HBM(Cx)], cerebellum [HBM(Cb)], and hippocampus [HBM(H)] probed with N52A/42 TC supe.](http://www.antibodiesinc.com/cdn/shop/files/73-127-n52a-42-wb-1_{width}x.jpg?v=1703185459)
Immunoblot against crude membranes prepared from whole rat (RBM) and mouse (MBM) brain and human cerebral cortex [HBM(Cx)], cerebellum [HBM(Cb)], and hippocampus [HBM(H)] probed with N52A/42 TC supe.
![Immunoblot against crude membranes prepared from whole rat (RBM) and mouse (MBM) brain and human cerebral cortex [HBM(Cx)], cerebellum [HBM(Cb)], and hippocampus [HBM(H)] probed with N52A/42 TC supe.](http://www.antibodiesinc.com/cdn/shop/files/73-127-n52a-42-wb-1_1024x.jpg?v=1703185459)
Human, Mouse, Non-Human Primate, Rat
ICC, IHC, IP, WB
Mouse
Product Specific References for Applications and Species
- Immunocytochemistry: Human | Mouse
- Western Blot: Human | Mouse | Non-human Primate | Rat | Worm
- Additional Publications: Unspecified
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 32432379 | 1:1000 | Acin‐Perez, R., et al. 2020. A novel approach to measure mitochondrial respiration in frozen biological samples. The EMBO Journal, e104073. |
| 28057766 | not listed | Steffen, J., et al. 2017. Rapid degradation of mutant SLC25A46 by the ubiquitin-proteasome system results in MFN1/2-mediated hyperfusion of mitochondria. Molecular and Cellular Biology, 600-612. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 37722569 | 1:50 | Bueno, D, et al. 2023. NECAB2 is an endosomal protein important for striatal function. Free Radical Biology & Medicine, 643-656. |
| 32235845 | not listed | Zieger, H.L., et al. 2020. Disease-associated synaptic scaffold protein CNK2 modulates PSD size and influences localisation of the regulatory kinase TNIK. Scientific Reports, 5709. |
| 30590615 | 1:200 | Dong, Y.N., et al. 2019. GRP75 overexpression rescues frataxin deficiency and mitochondrial phenotypes in Friedreich ataxia cellular models. . Human molecular genetics, 1594-1607. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 35662277 | 1:1000 | Wolf, C., et al. 2022. GDAP1 loss of function inhibits the mitochondrial pyruvate dehydrogenase complex by altering the actin cytoskeleton. Communications Biology, 541. |
| 33730581 | not listed | Acoba, M.G., et al. 2021. The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism. Cell Reports, 108869. |
| 31325344 | 1:5000 | Jurek, B., et al. 2019. Human gestational N-methyl-d-aspartate receptor autoantibodies impair neonatal murine brain function. Annals of Neurology, 656-670. |
| 29253589 | 1:1000 | Blunsom, N.J., et al. 2018. Mitochondrial CDP-diacylglycerol synthase activity is due to the peripheral protein, TAMM41 and not due to the integral membrane protein, CDP-diacylglycerol synthase 1. Biochimica et Biophysica Acta. Molecular and Cell Biology of Lipids, 284-298. |
| 28404750 | not listed | Lu, Y.W., et al. 2017. Human adenine nucleotide translocases physically and functionally interact with respirasomes. Molecular and Cellular Biology, 1489-1506. |
| 27616904 | not listed | Hwang, J.Y., et al. 2016. Proteomic analysis reveals that the protective effects of ginsenoside Rb1 are associated with the actin cytoskeleton in β-amyloid-treated neuronal cells. Journal of Ginseng Research, 278-284. |
| 27543974 | not listed | Wan, J., et al. 2016. Loss of function of SLC25A46 causes lethal congenital pontocerebellar hypoplasia. Brain, 2877-2890. |
| 26644354 | not listed | Gong, B., et al. 2016. Developing high-quality mouse monoclonal antibodies for neuroscience research - approaches, perspectives and opportunities. New Biotechnology, 551-564. |
| 26110647 | not listed | Cáceres, M., et al. 2015. TRPM4 Is a Novel Component of the Adhesome Required for Focal Adhesion Disassembly, Migration and Contractility.. PLoS One, e0130540. |
| 25231975 | not listed | Cerda, O., et al. 2015. Casein kinase-mediated phosphorylation of serine 839 is necessary for basolateral localization of the Ca²⁺-activated non-selective cation channel TRPM4.. Pflügers Archiv, 1723-1732. |
| 25027320 | 1 : 1000 | Ramirez, A., et al. 2014. SUCLG2 identified as both a determinator of CSF Aβ1-42 levels and an attenuator of cognitive decline in Alzheimer''s disease.. Human Molecular Genetics, 6644-6658. |
| 24001768 | not listed | Runne, C., et al. 2013. PLEKHG2 promotes heterotrimeric G protein βγ-stimulated lymphocyte migration via Rac and Cdc42 activation and actin polymerization.. Molecular and Cellular Biology, 4294-4307. |
| 23269666 | not listed | Arango, N.A., et al. 2013. Meiosis I arrest abnormalities lead to severe oligozoospermia in meiosis 1 arresting protein (M1ap)-deficient mice.. Biology of Reproduction, 76. |
| 21712386 | not listed | Cerda, O., et al. 2011. Activity-dependent phosphorylation of neuronal Kv2.1 potassium channels by CDK5.. The Journal of Biological Chemistry, 28738-28748. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 39603281 | 1:1000 | Hawkins, N.A., et al. 2024. Novel mouse model of alternating hemiplegia of childhood exhibits prominent motor and seizure phenotypes. Neurobiology of Disease, 106751. |
| 38485094 | 1ug/ml | Kang, SK, et al. 2024. Altered neurological and neurobehavioral phenotypes in a mouse model of the recurrent KCNB1-p.R306C voltage-sensor variant. Neurobiology of Disease, 106470. |
| 35943566 | not listed | van der Kooij, M.A., et al. 2022. Chronic Social Stress Disrupts the Intracellular Redistribution of Brain Hexokinase 3 Induced by Shifts in Peripheral Glucose Levels. Journal of Molecular Medicine, 1441-1453. |
| 35417922 | 1:1000 | Echevarria-Cooper, D.M., et al. 2022. Cellular and behavioral effects of altered NaV1.2 sodium channel ion permeability in Scn2aK1422E mice. Human molecular genetics, 2964-2988. |
| 34822817 | 1ug/ml | Anderson, L.L., et al. 2022. The endocannabinoid system impacts seizures in a mouse model of Dravet syndrome. Neuropharmacology, 108897. |
| 34086081 | 1 ug/mL | Hawkins, N.A., et al. 2021. Gabra2 is a genetic modifier of Dravet syndrome in mice. Mammalian Genome, 350-363. |
| 33502442 | 1:10 (supe) | Fortenbach, C., et al. 2021. Loss of the K+ channel Kv2.1 greatly reduces outward dark current and causes ionic dysregulation and degeneration in rod photoreceptors. Journal of General Physiology, . |
| 33436529 | not listed | Wang, Y.Q., et al. 2021. AMPA and NMDA Receptor Trafficking at Cocaine-Generated Synapses. Journal of Neuroscience, 1996-2011. |
| 33132203 | 1 ug/mL | Hawkins, N.A., et al. 2021. Epilepsy and neurobehavioral abnormalities in mice with a dominant-negative KCNB1 pathogenic variant. Neurobiology of Disease, 105141. |
| 30347190 | 1:1000 | Hawkins, N.A., et al. 2019. Gene expression profiling in a mouse model of Dravet syndrome. Experimental Neurology, 247-256. |
| 30586380 | not listed | Favaro, P.D., et al. 2018. An opposing function of paralogs in balancing developmental synapse maturation. Plos Biology, e2006838. |
| 29976627 | 1:1000 | Shepherd, A.J., et al. 2018. Angiotensin II Triggers Peripheral Macrophage-to-Sensory Neuron Redox Crosstalk to Elicit Pain. Journal of Neuroscience, 7032-7057. |
| 29403353 | not listed | Bishop HI, et al. 2018. Kv2 Ion Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons.. Frontiers in Molecular Neuroscience, 1. |
| 28490751 | 1:1000 | Anderson, L.L., et al. 2017. Unexpected Efficacy of a Novel Sodium Channel Modulator in Dravet Syndrome. Scientific Reports, 1682. |
| 28344592 | not listed | Larimore, J., et al. 2017. Dysbindin Deficiency Modifies the Expression of GABA Neuron and Ion Permeation Transcripts in the Developing Hippocampus.. Frontiers in Genetics, 28. |
| 28077487 | 1:1000 | Shukla, A., et al. 2017. Calcium‐permeable AMPA receptors and silent synapses in cocaine‐conditioned place preference. The EMBO journal, 36(4), pp.458-474.. The EMBO journal, 458-474. |
| 27768696 | 1:1000 | Hawkins, N.A., et al. 2016. Fine Mapping of a Dravet Syndrome Modifier Locus on Mouse Chromosome 5 and Candidate Gene Analysis by RNA-Seq. PLOS Genetics, e1006398. |
| 27147658 | 1:2000 | Shutov, L.P., et al. 2016. The Complement System Component C5a Produces Thermal Hyperalgesia via Macrophage-to-Nociceptor Signaling That Requires NGF and TRPV1. Journal of Neuroscience, 5055-5070. |
| 27147658 | 1:2000 | Shutov, L.P., et al. 2016. The Complement System Component C5a Produces Thermal Hyperalgesia via Macrophage-to-Nociceptor Signaling That Requires NGF and TRPV1. Journal of Neuroscience, 5055-5070. |
| 26644354 | not listed | Gong, B., et al. 2016. Developing high-quality mouse monoclonal antibodies for neuroscience research - approaches, perspectives and opportunities. New Biotechnology, 551-564. |
| 26538660 | 1 : 20 | Bishop, H.I., et al. 2015. Distinct Cell- and Layer-Specific Expression Patterns and Independent Regulation of Kv2 Channel Subtypes in Cortical Pyramidal Neurons.. Journal of Neuroscience, 14922-14942. |
| 26442584 | not listed | Cobb, M.M., et al. 2015. Cell Cycle-dependent Changes in Localization and Phosphorylation of the Plasma Membrane Kv2.1 K+ Channel Impact Endoplasmic Reticulum Membrane Contact Sites in COS-1 Cells.. The Journal of Biological Chemistry, 29189-29201. |
| 24795561 | 1:1000 | Joaquin N. Lugo, et al. 2014. Deletion of PTEN produces autism-like behavioral deficits and alterations in synaptic proteins.. Frontiers in Molecular Neuroscience, 27. |
| 24494598 | not listed | Speca, D.J., et al. 2014. Deletion of the Kv2.1 delayed rectifier potassium channel leads to neuronal and behavioral hyperexcitability.. Genes, Brain and Behavior, 394-408. |
| 24068818 | not listed | Krüger, J.M., et al. 2013. Differential roles of postsynaptic density-93 isoforms in regulating synaptic transmission.. Journal of Neuroscience, 15504-15517. |
| 23946397 | not listed | Bonnet, S.A., et al. 2013. Synaptic state-dependent functional interplay between postsynaptic density-95 and synapse-associated protein 102.. Journal of Neuroscience, 13398-13409. |
| 23223301 | not listed | Kummer M.P., et al. 2012. Mrp14 deficiency ameliorates amyloid β burden by increasing microglial phagocytosis and modulation of amyloid precursor protein processing.. Journal of Neuroscience, 17824-17829. |
| 23012412 | not listed | Winters, B.D., et al. 2012. Cannabinoid receptor 1-expressing neurons in the nucleus accumbens. PNAS USA, E2717-E2725. |
| 22933780 | 1:1000 | Loo, L., et al. 2012. The C-type natriuretic peptide induces thermal hyperalgesia through a noncanonical Gβγ-dependent modulation of TRPV1 channel.. Journal of Neuroscience, 11942-11955. |
| 20691904 | 1:1000 | Wang, G., et al. 2010. PNPASE regulates RNA import into mitochondria.. Cell, 456-467. |
| Western Blot: Non-human Primate | ||
| PMID | Dilution | Publication |
| 26503721 | not listed | Thiffault, I., et al. 2015. A novel epileptic encephalopathy mutation in KCNB1 disrupts Kv2.1 ion selectivity, expression, and localization.. The Journal of General Physiology, 399-410. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 26442584 | not listed | Cobb, M.M., et al. 2015. Cell Cycle-dependent Changes in Localization and Phosphorylation of the Plasma Membrane Kv2.1 K+ Channel Impact Endoplasmic Reticulum Membrane Contact Sites in COS-1 Cells.. The Journal of Biological Chemistry, 29189-201. |
| 22977251 | 1:2000 | Ulrich, J.D., et al. 2012. Distinct activation properties of the nuclear factor of activated T-cells (NFAT) isoforms NFATc3 and NFATc4 in neurons.. The Journal of Biological Chemistry, 37594-609. |
| Western Blot: Worm | ||
| PMID | Dilution | Publication |
| 25898113 | 1:20 (supe) | Lawrence, K.S., et al. 2015. DNA damage response and spindle assembly checkpoint function throughout the cell cycle to ensure genomic integrity.. PLOS Genetics, e1005150. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 28322444 | Vicente, P.C., et al. 2018. Identification and characterization of site-specific N-glycosylation in the potassium channel Kv3.1b. Journal of Cell Physiology, 549-558. | |