Our Anti-Mortalin/GRP75 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N52A/42. It detects human, mouse, and rat Mortalin/GRP75, and is purified by Protein A chromatography. It is great for use in IHC, ICC.
Mitochondrial stress-70 protein or Mortalin/GRP75 is encoded by the gene HSPA9. Mortalin is a chaperone protein and plays a role in cell proliferation, stress response and mitochondria maintenance. It is widely expressed in tissues in mitochondria, endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. Diseases associated with this gene include Even-Plus Syndrome and Sideroblastic Anemia 4.
Purified by Protein A chromatography
0.5 mg/mL
Monoclonal
N52A/42
IgG1
ICC, IHC
Mouse
Hspa9 Grp75 Hsp74 Hspa9a
75 kDa
Identified as off-target mAb in screen for anti-Salm2 NeuroMabs (immunogen was fusion protein amino acids 551-766 of mouse SALM2). Target identified as Mortalin/Grp75 by LC-MS/MS analysis of protein immunopurified from rat brain using N52A/42.
Human, Mouse, Non-Human Primate, Rat
AB_2939472
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
PBS with 0.09% azide
FL490 Ex: 491 nm, Em: 515 nm
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
