Our Semaphorin-3E (N-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Semaphorin-3E (N-terminal region) and is antigen affinity purified. It is great for use in WB.
Western blot analysis of rat PC12 cells (lanes 1 & 2) and human PC-3 cells (lanes 3 & 4). The blots were probed with anti-Sema-3E (N-terminal region) antibody in absence (lanes 1 & 3) or presence of Sema-3E (N-terminal region) blocking peptide (lanes 2 & 4).
The Semaphorin family of axon guidance molecules includes secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 3E (Sema-3E) is a class III secreted semaphorin that binds Plexin D1 receptor, and has putative roles in axon guidance, angiogenesis, and cancers. Mutations in the Sema-3E gene are associated with CHARGE syndrome, a disorder that has nerve and cardiovascular abnormalities. The Sema-3E protein is expressed as full length (87 kDa) and furin-dependent processed forms (61 and 25 kDa). These forms are observed as monomers, as well as homo- and hetero-dimers. Sema-3E protein is frequently expressed in human cancer cell lines and in solid tumors from breast cancer patients. The expression of Sema-3E in mammary adenocarcinoma cells induces the ability to form experimental lung metastasis, and this activity requires furin-dependent production of the 61 kDa Sema-3E protein.
Antigen Affinity Purified
Polyclonal
IgG
WB
Rabbit
SEMA3E
61/87
Sema-3E (N-terminal region) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminal region of human Sema-3E. This sequence is well conserved in rat, mouse, and chicken Sema-3E, and has low homology to other semaphorin family members.
Chicken, Human, Mouse, Rat
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
PBS + 0.05% NaN3
WB: 1:1000
Unconjugated
The antibody detects 87 and 61 kDa bands corresponding to the full-length and furin-proccessed forms of Sema-3E in human PC-3 and rat PC12 cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Mazzotta, C, et al. 2015. Plexin-D1/Semaphorin 3E pathway may contribute to dysregulation of vascular tone control and defective angiogenesis in systemic sclerosis. Arthritis Research and Therapy, 221.
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