Our Anti-Synaptotagmin-12 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N277/7. It detects mouse and rat Synaptotagmin-12, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N277/7 (left) and N86/8 (right).
Synaptotagmins are a family of calcium sensing, membrane trafficking proteins. 17 different synaptotagmins have been identified in mammals. Synaptotagmin 12 differs from classical synaptotagmins in that it does not bind Ca(2+). In synaptic vesicles, synaptotagmin-12 forms a complex with synaptotagmin-1 that prevents synaptotagmin-1 from interacting with SNARE complexes (Maximov A. et al., 2007).
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N277/7
IgG1
ICC, IHC, WB
Mouse
Syt12
45 kDa (plus others)
Fusion protein amino acids 168-255 (cytoplasmic C2A domain) of mouse Synaptotagmin-12 (also known as Syt12, Synaptotagmin XII, SytXII, Synaptotagmin-related gene 1 protein, Srg1 and Sytr1, accession number Q920N7) produced recombinantly in E. Coli
Mouse, Rat
AB_2315940
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Unconjugated
Does not cross-react with Synaptotagmin-6 (or other Synaptotagmins)
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Ramachandran, B., et al. 2015. Ethanol inhibits long-term potentiation in hippocampal CA1 neurons, irrespective of lamina and stimulus strength, through neurosteroidogenesis.. Hippocampus, 106-118.
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