Anti-Synaptotagmin-12 Antibody (N277/7)

Name: Anti-Synaptotagmin-12 Antibody
Brand: NeuroMab
SKU: 75-304
Price: 319.0 USD
Availability: InStock

1 Citation

Our Anti-Synaptotagmin-12 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N277/7. It detects mouse and rat Synaptotagmin-12, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N277/7 (left) and N86/8 (right).

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-304

Product Details

Target

Synaptotagmin-12

Target Description

Synaptotagmins are a family of calcium sensing, membrane trafficking proteins. 17 different synaptotagmins have been identified in mammals. Synaptotagmin 12 differs from classical synaptotagmins in that it does not bind Ca(2+). In synaptic vesicles, synaptotagmin-12 forms a complex with synaptotagmin-1 that prevents synaptotagmin-1 from interacting with SNARE complexes (Maximov A. et al., 2007).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N277/7

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Syt12

Molecular Weight

45 kDa (plus others)

Antigen

Fusion protein amino acids 168-255 (cytoplasmic C2A domain) of mouse Synaptotagmin-12 (also known as Syt12, Synaptotagmin XII, SytXII, Synaptotagmin-related gene 1 protein, Srg1 and Sytr1, accession number Q920N7) produced recombinantly in E. Coli

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2315940

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with Synaptotagmin-6 (or other Synaptotagmins)

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Synaptotagmin-12 (Synaptotagmin XII) (SytXII)

UniProt Details

UniProt (Human): Q8IV01
UniProt (Immunogen Species): Q920N7

Ramachandran B1, Ahmed S, Zafar N, Dean C. (), 'Ethanol inhibits long-term potentiation in hippocampal CA1 neurons, irrespective of lamina and stimulus strength, through neurosteroidogenesis..' Hippocampus. 10.1002/hipo.22356.

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Anti-Synaptotagmin-12 Antibody (N277/7)

SKU: 75-304

Product Details

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