Anti-SynCAM4 Antibody (N244/5)
Our Anti-SynCAM4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N244/5. It is KO validated, detects human, mouse, and rat SynCAM4, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Adult rat sciatic nerve immunofluorescence staining: paranodes labeled with N244/5 (red) surround nodes of Ranvier labeled with Beta-IV spectrin (green) (image on right is cross-section of a similar image of left). Images courtesy of Matt Rasband (Baylor College of Medicine).
Roll over image to zoom in
Click on image to zoom
Human, Mouse, Rat
ICC, IHC, WB
Mouse
KO Validated
Product Specific References for Applications and Species
- Immunocytochemistry: Human | Mouse | Rat
- Immunohistochemistry: Mouse
- Light Microscopy: Mouse
- Western Blot: Human | Mouse | Rat
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 25893857 | 1:100 | Yamana, S., et al. 2015. The Cell Adhesion Molecule Necl-4/CADM4 Serves as a Novel Regulator for Contact Inhibition of Cell Movement and Proliferation.. PLoS One, e0124259. |
| 23611113 | not listed | Sugiyama, H., et al. 2013. Interaction of Necl-4/CADM4 with ErbB3 and integrin α6 β4 and inhibition of ErbB2/ErbB3 signaling and hemidesmosome disassembly.. Genes Cells, 519-528. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 36458527 | 1:300 | Nozawa, O., et al. 2023. Necl2/3-mediated mechanism for tripartite synapse formation. Development, dev200931. |
| 36458527 | 1:300 | Nozawa, O., et al. 2022. A Novel Necl-2/3-mediated Mechanism for Tripartite Synapse Formation. Development, . |
| 31451613 | not listed | Elazar, N., et al. 2019. Coordinated internodal and paranodal adhesion controls accurate myelination by oligodendrocytes. Journal of Cell Biology, 2887-2895. |
| 30389906 | 1:500 | Shacham-Silverberg, V., et al. 2018. Phosphatidylserine is a marker for axonal debris engulfment but its exposure can be decoupled from degeneration. Cell Death and Disease, 1116. |
| 30389906 | 1:500 | Shacham-Silverberg, V., et al. 2018. Phosphatidylserine is a marker for axonal debris engulfment but its exposure can be decoupled from degeneration. Cell Death and Disease, 1116. |
| 25362471 | not listed | Chang, K.J., et al. 2014. Glial ankyrins facilitate paranodal axoglial junction assembly.. Nature Neuroscience, 1673-81. |
| 24127433 | 1:400 | Minis, A., et al. 2014. Subcellular transcriptomics-dissection of the mRNA composition in the axonal compartment of sensory neurons.. Developmental Neurobiology, 365-381. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 26644354 | not listed | Gong, B., et al. 2016. Developing high-quality mouse monoclonal antibodies for neuroscience research–approaches, perspectives and opportunities. New Biotechnology, 551-564. |
| 21518878 | not listed | Susuki, K., et al. 2011. Schwann cell spectrins modulate peripheral nerve myelination.. PNAS: USA, 8009-8014. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 35445918 | 1:100 | Jordans, S., et al. 2022. Age-Dependent Increase in Schmidt-Lanterman Incisures and a Cadm4-Associated Membrane Skeletal Complex in Fatty Acid 2-hydroxylase Deficient Mice: a Mouse Model of Spastic Paraplegia SPG35. Molecular Neurobiology, 3969-3979. |
| 30551998 | not listed | Elazar, N., et al. 2019. Axoglial Adhesion by Cadm4 Regulates CNS Myelination. Neuron, 224-231. |
| 29282769 | not listed | Bang., M.L, et al. 2018. Glial M6B stabilizes the axonal membrane at peripheral nodes of Ranvier. Glia, 801-812. |
| 23825401 | not listed | Golan, N., et al. 2013. Genetic deletion of Cadm4 results in myelin abnormalities resembling Charcot-Marie-Tooth neuropathy.. Journal of Neuroscience, 10950-10961. |
| Light Microscopy: Mouse | ||
| PMID | Dilution | Publication |
| 32452538 | 1:300 | Shiotani, H., et al. 2021. Nectin-2α is localized at cholinergic neuron dendrites and regulates synapse formation in the medial habenula. Journal of Comparative Neurology, 450-477. |
| 29524214 | 1:300 | Shiotani, H., et al. 2018. Localization of nectin-2α at the boundary between the adjacent somata of the clustered cholinergic neurons and its regulatory role in the subcellular localization of the voltage-gated A-type K+ channel Kv4.2 in the medial habenula. Journal of Comparative Neurology, 1527-1549. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 28601637 | not listed | Yamana, S., et al. 2017. Necl-4 enhances the PLCγ-c-Raf-MEK-ERK pathway without affecting internalization of VEGFR2. Biochemical and Biophysical Research Communication, 169-175. |
| 25893857 | 1:1000 | Yamana, S., et al. 2015. The Cell Adhesion Molecule Necl-4/CADM4 Serves as a Novel Regulator for Contact Inhibition of Cell Movement and Proliferation.. PLoS One, e0124259. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 36458527 | 1:1000 | Nozawa, O., et al. 2023. Necl2/3-mediated mechanism for tripartite synapse formation. Development, dev200931. |
| 36458527 | 1:1000 | Nozawa, O., et al. 2022. A Novel Necl-2/3-mediated Mechanism for Tripartite Synapse Formation. Development, . |
| 35445918 | 1:250 | Jordans, S., et al. 2022. Age-Dependent Increase in Schmidt-Lanterman Incisures and a Cadm4-Associated Membrane Skeletal Complex in Fatty Acid 2-hydroxylase Deficient Mice: a Mouse Model of Spastic Paraplegia SPG35. Molecular Neurobiology, 3969-3979. |
| 33215680 | 1:250 | Hardt, R., et al. 2021. Decreased turnover of the CNS myelin protein Opalin in a mouse model of hereditary spastic paraplegia 35. Human Molecular Genetics, 3616-3630. |
| 31262725 | 1:500 | Trotter, J.H., et al. 2019. Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters. Journal of Cell Biology, 2677-2698. |
| 31262725 | 1:500 | Trotter, J.H., et al. 2019. Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters. Journal of Cell Biology, 2677-2698. |
| 30551998 | not listed | Elazar, N., et al. 2019. Axoglial Adhesion by Cadm4 Regulates CNS Myelination. Neuron, 224-231. |
| 30357511 | 1:1000 | Saitoh, Y., et al. 2019. The membrane palmitoylated protein, MPP6, is involved in myelin formation in the mouse peripheral nervous system. Histochemistry and Cell Biology, 385-394. |
| 23825401 | not listed | Golan, N., et al. 2013. Genetic deletion of Cadm4 results in myelin abnormalities resembling Charcot-Marie-Tooth neuropathy.. Journal of Neuroscience, 10950-10961. |
| 23700466 | not listed | Zhu,Y., et al. 2013. Necl-4/SynCAM-4 is expressed in myelinating oligodendrocytes but not required for axonal myelination.. PLoS One, e64264. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 27658374 | 1:1000 | Chen, M.S., et al. 2016. Cadm3 (Necl-1) interferes with the activation of the PI3 kinase/Akt signaling cascade and inhibits Schwann cell myelination in vitro. Glia, 2247-2262. |
