Anti-Beta1-Spectrin Antibody (N385/21)
Our Anti-Beta1-spectrin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N385/21. It detects human, mouse, and rat Beta1-spectrin, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Immunofluorescence staining of optic nerve from adult Beta4-spectrin wild-type (WT) and conditional knockout (cKO) mice with N385/21 (Beta1- spectrin, red), Beta4- spectrin antibody (green) and Neurofascin antibody (blue). Images courtesy of Cheng-Hsin Liu and Matt Rasband (Baylor College of Medicine).
Product Specific References for Applications and Species
- Immunocytochemistry: Human | Mouse
- Immunohistochemistry: Mouse
- Immunoprecipitation: Mouse
- Western Blot: Mouse
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 36543363 | 1:50 | Hou, M., et al. 2022. Molecular Resolution Mapping of Erythrocyte Cytoskeleton by Ultrastructure Expansion Single-Molecule Localization Microscopy. Small Methods, e2201243. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 38977673 | 1:200 | Ghisleni, A, et al. 2024. Mechanically induced topological transition of spectrin regulates its distribution in the mammalian cell cortex. Nature Communications, 5711. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 34785580 | 1:250 | Stevens, S.R., et al. 2022. Ankyrin-R Links Kv3.3 to the Spectrin Cytoskeleton and Is Required for Purkinje Neuron Survival. Journal of Neuroscience, 2-15. |
| 34289389 | not listed | Zhang, C., et al. 2021. Ankyrin-dependent Na+ channel clustering prevents neuromuscular synapse fatigue. Current Biology, 3810-3819. |
| 34180393 | 1:250 | Stevens, S.R., et al. 2021. Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3. 1b K+ channels. . Elife, e66491. |
| 32425157 | 1:500 | Liu, C.H., et al. 2020. β spectrin-dependent and domain specific mechanisms for Na + channel clustering. Elife, e56629. |
| 32052742 | 1:500 | Liu, C.H., et al. 2020. Nodal β spectrins are required to maintain Na + channel clustering and axon integrity. Elife, e52378. |
| Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 34180393 | 5ul | Stevens, S.R., et al. 2021. Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3. 1b K+ channels. . Elife, e66491. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 34180393 | 1:1000 | Stevens, S.R., et al. 2021. Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3. 1b K+ channels. . Elife, e66491. |
| 32052742 | 1:1000 | Liu, C.H., et al. 2020. Nodal β spectrins are required to maintain Na + channel clustering and axon integrity. Elife, e52378. |