Anti-Beta4-Spectrin Antibody (N393/76)

14 Citations

Our Anti-Beta4-spectrin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N393/76. It detects human, mouse, and rat Beta4-spectrin, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat cerebral cortex immunohistochemistry.
Adult rat cerebral cortex immunohistochemistry.
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Adult rat cerebral cortex immunohistochemistry.
Hippocampus immunohistochemistry with antigen retrieval via pepsin pretreatment showing axon initial segment and nodal staining.
Higher magnification of corpus callosum and CA1.
Immunoblot against extracts of COS cells transiently transfected with tagged Beta-spectrins or untagged Kv2.1 plasmid probed with N393/76 (left) or 19E10 (right) TC supe.
Immunocytochemistry for α1, α2, and β4 spectrin (cat. 75-377, 1:500) carried out in fixed and permeabilized DIV 21 primary cultured pHα2 mouse neurons. Image from publication CC-BY-4.0. PMID:36604600
Confocal images of primary hippocampal neurons stained with phalloidin-647 (F-actin marker) and with an antibody against beta4-spectrin (Antibodies Inc; Cat

Human, Mouse, Rat
ICC, IHC, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet

SKU: 75-377

Volume: 100 µL
Price:
$385.00
Ships: 1-2 business days

Product Details

Beta4-spectrin
Spectrin beta chain, non-erythrocytic 4, or ß4 spectrin, is a member of the spectrin family of cytoskeletal proteins. It is found in the cytoskeleton and cell cortex of cells in the brain and pancreatic islets. It is also expressed in skeletal muscule. ß4 spectrin functions as a scaffold protein that links the actin cytoskeleton to the plasma membrane. It is involved in cell shape and organization. Mutations in the ß4 spectrin gene are associated with congenital myopathy, and neurodevelopmental disorder with hypotonia, neuropathy and deafness (NEDHND).
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N393/76
IgG2b
ICC, IHC, WB
Mouse
SPTBN4 KIAA1642 SPTBN3
>200 kDa
Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin (accession number Q9H254) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2315818
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:1000
IHC: 1:250
ICC: 1:500
Unconjugated
Does not cross-react with other Beta-spectrins
Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Spectrin beta chain, non-erythrocytic 4 (Beta-IV spectrin) (Spectrin, non-erythroid beta chain 3)
Q9H254

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
399202951:200Di Re, J, et al. 2025. βIV spectrin abundancy, cellular distribution and sensitivity to AKT/GSK3 regulation in schizophrenia. Molecular Psychiatry, .
398049911:500Wang, H, et al. 2025. The Primary Cilia are Associated with the Axon Initial Segment in Neurons. Advanced Science, e2407405.
Immunocytochemistry: Mouse
PMID Dilution Publication
394158471:500Li, Y, et al. 2024. Alterations in the axon initial segment plasticity is involved in early pathogenesis in Alzheimer's disease. MedComm, e768.
385256001:500Konietzny, A, et al. 2024. Efficient axonal transport of endolysosomes relies on the balanced ratio of microtubule tyrosination and detyrosination. Journal of Cell Science, .
366046001:500Smalley, J.L., et al. 2023. Spectrin-beta 2 Facilitates the Selective Accumulation of GABAA Receptors at Somatodendritic Synapses. Communications Biology, 11.
354119841:500Wang, Y., et al. 2022. Glucose Increases the Length and Spacing of the Lattice Structure of the Axon Initial Segment. Microscopy Research and Technique, 2679-2691.
34534742not listedWang, Y., et al. 2021. The largest isoform of Ankyrin-G is required for lattice structure of the axon initial segment. Biochemical and biophysical research communications, 28-34.
32125730not listedJarjour, A.A., et al. 2020. The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2. Glia, 1840-1858.
29398366not listedJacko, M., et al. 2018. Rbfox Splicing Factors Promote Neuronal Maturation and Axon Initial Segment Assembly. Neuron, 853-868.
Immunohistochemistry: Human
PMID Dilution Publication
399202951:500Di Re, J, et al. 2025. βIV spectrin abundancy, cellular distribution and sensitivity to AKT/GSK3 regulation in schizophrenia. Molecular Psychiatry, .
Immunohistochemistry: Mouse
PMID Dilution Publication
315911561:200Sozmen, E.G., et al. 2019. White matter stroke induces a unique oligo-astrocyte niche that inhibits recovery. Journal of Neuroscience, 39(47), pp.9343-9359.. Journal of Neuroscience, 9343-9359.
315329181:200Di Re, J., et al. 2019. Imaging of the Axon Initial Segment. Current Protocols in Neuroscience, e78.
Immunohistochemistry: Rat
PMID Dilution Publication
327363741:250Werginz, P., et al. 2020. The relationship between morphological properties and thresholds to extracellular electric stimulation in α RGCs. Journal of Neural Engineering, 45015.
325531161:250Hanemaaijer, N.A., et al. 2020. Ca 2+ entry through Na V channels generates submillisecond axonal Ca 2+ signaling. Elife, e54566.
Western Blot: Mouse
PMID Dilution Publication
366046001:1000Smalley, J.L., et al. 2023. Spectrin-beta 2 Facilitates the Selective Accumulation of GABAA Receptors at Somatodendritic Synapses. Communications Biology, 11.
31910261not listedLee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033.
299565861:500Dash, B., et al. 2018. Nonmuscle myosin II isoforms interact with sodium channel alpha subunits. . Molecular pain, .
Western Blot: Rat
PMID Dilution Publication
287857241:1000Kneynsberg, A., et al. 2017. Aging Does Not Affect Axon Initial Segment Structure and Somatic Localization of Tau Protein in Hippocampal Neurons of Fischer 344 Rats. eNeuro, .

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