Anti-BKbeta4 K+ Channel Antibody (L18A/3)

Name: Anti-BKbeta4 K+ Channel Antibody
Brand: NeuroMab
SKU: 75-086
Price: 319.0 USD
Availability: InStock

4 Citations

Our Anti-BKbeta4 K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L18A/3. It detects human, mouse, and rat BKbeta4 K+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with BKBeta4 or Kv2.1 plasmids and probed with L18A/3 pure IgG at 10 mg/mL (left panel) or K89/41 TC supe (right panel).

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-086

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

BKbeta4 K+ channel

Target Description

Calcium-activated potassium channel subunit beta-4, Potassium Calcium-Activated Channel Subfamily M Regulatory Beta Subunit 4 or BKBeta4 is encoded by the gene KCNMB4. BKbeta4 can bind to KCNMA1 to form a channel in the calcium activated large conductance (MaxiK channel) family. BKbeta4 acts to modulate the calcium sensitivity and gating kinetics of KCNMA1. It is expressed in brain and CNS.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L18A/3

Isotype

IgG2a

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Kcnmb4

Molecular Weight

24 kDa

Antigen

Fusion protein amino acids 45-167 (entire extracellular domain) of mouse BKBeta4 (accession number Q9JIN6) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2131507

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.16

Dilution Ranges

WB: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity against BKBeta1, BKBeta2 or BKBeta3

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Calcium-activated potassium channel subunit beta-4 (BK channel subunit beta-4) (BKbeta4) (Calcium-activated potassium channel, subfamily M subunit beta-4) (Charybdotoxin receptor subunit beta-4) (K(VCA)beta-4) (Maxi K channel subunit beta-4) (Slo-beta-4)

UniProt Details

UniProt (Human): Q86W47
UniProt (Immunogen Species): Q9JIN6

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Western Blot:

Immunocytochemistry: Mouse
PMID	Dilution	Publication
23504458	1:300	Chen, L., et al. 2013. Palmitoylation of the β4-subunit regulates surface expression of large conductance calcium-activated potassium channel splice variants. The Journal of biological chemistry, 13136-13144.
22438928	1:500	Shruti, S., et al. 2012. The brain-specific Beta4 subunit downregulates BK channel cell surface expression. PLoS One, e33429.

Western Blot: Mouse
PMID	Dilution	Publication
30271963	1:1000	Yu, D., et al. 2018. BK channel deacetylation by SIRT1 in dentate gyrus regulates anxiety and response to stress. Communications biology, 82.
22607137	1:200	Zhang, X., et al. 2012. Inflammation-induced changes in BK(Ca) currents in cutaneous dorsal root ganglion neurons from the adult rat. Molecular pain, 37.

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Anti-BKbeta4 K+ Channel Antibody (L18A/3)

SKU: 75-086

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-BKbeta4 K+ Channel Antibody

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