Anti-GABA-A-R, Beta1 Antibody (N96/55)

Name: Anti-GABA-A-R, Beta1 Antibody
Brand: NeuroMab
SKU: 75-137
Price: 319.0 USD
Availability: InStock

8 Citations

Our Anti-GABA(A)R, Beta1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N96/55. It detects human and mouse GABA(A)R, Beta1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.

Immunofluorescence of cultured hippocampal neurons with N96/55 (red), GABA- A-R-Gamma2 rabbit polyclonal (green) and GAD (blue). Images courtesy of Angel de Blas (University of Connecticut).

Adult rat brain membrane immunoblot.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-137

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

GABA(A)R, Beta1

Target Description

Gamma-aminobutyric acid receptor subunit beta-1 or -GABA AR-Beta1 is encoded by the gene GABRB1 GABAA receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR alpha 5 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR-Beta1 is primarily expressed in brain at the cell junction, synapse,and the postsynaptic cell membrane. Diseases associated with GABRB1 include early infantile epileptic encephalopathy 43, neurotic disorder, schizophrenia and autism.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N96/55

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Gabrb1

Molecular Weight

55 kDa

Antigen

Fusion protein amino acids 327-450 of mouse GABA-A-R-Beta1 (accession number P50571) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2109406

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.64

Dilution Ranges

WB: 1:1000
IHC: 1:200
ICC: 1:200

Conjugate

Unconjugated

Specificity

No cross-reactivity against GABA-A-R-Beta2 or -Beta3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Gamma-aminobutyric acid receptor subunit beta-1 (GABA(A) receptor subunit beta-1)

UniProt Details

UniProt (Human): P18505
UniProt (Immunogen Species): P50571

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Rat
Immunoprecipitation: Mouse
Western Blot: Mouse | Rat

Immunocytochemistry: Human
PMID	Dilution	Publication
24920620	not listed	Ohkawa, T., et al. 2014. Identification and characterization of GABA(A) receptor autoantibodies in autoimmune encephalitis.. Journal of Neuroscience, 8151-8163.

Immunohistochemistry: Rat
PMID	Dilution	Publication
24477962	1:5 (supe)	King, A.N., et al. 2014. A unique ion channel clustering domain on the axon initial segment of mammalian neurons. . Journal of Comparative Neurology, 2594-2608.
24477962	1:5	King, A.N., et al. 2014. A unique ion channel clustering domain on the axon initial segment of mammalian neurons.. Journal of Comparative Neurology, 2594.
22539847	1:1000	Eyre, M.D., et al. 2012. Setting the time course of inhibitory synaptic currents by mixing multiple GABA(A) receptor α subunit isoforms.. Journal of Neuroscience, 5853-5867.

Immunoprecipitation: Mouse
PMID	Dilution	Publication
38876310	not listed	Blednov, YA, et al. 2024. The PDE4 inhibitor apremilast modulates ethanol responses in Gabrb1-S409A knock-in mice via PKA-dependent and independent mechanisms. Neuropharmacology, 110035.

Western Blot: Mouse
PMID	Dilution	Publication
37078449	1:5000	Yokokawa, T., et al. 2023. Altered Expression of Synaptic Proteins and Adhesion Molecules in the Hippocampus and Cortex Following the Onset of Diabetes in Nonobese Diabetic Mice. Physiological Reports, e15673.
23744069	1:100	Zhou, C., et al. 2013. Altered cortical GABAA receptor composition, physiology, and endocytosis in a mouse model of a human genetic absence epilepsy syndrome.. The Journal of Biological Chemistry, 21458-21472.

Western Blot: Rat
PMID	Dilution	Publication
29706582	1:1000	Nguyen, Q.A., et al. 2018. The GABAA Receptor β Subunit Is Required for Inhibitory Transmission. Neuron, 718-725.

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Anti-GABA-A-R, Beta1 Antibody (N96/55)

SKU: 75-137

Product Details

Product Specific References for Applications and Species

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