Anti-KCC2 Antibody (N1/12)
Our Anti-KCC2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N1/12. It detects human, mouse, rat, and zebrafish KCC2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
Adult rat cerebellum immunohistochemistry.
Product Specific References for Applications and Species
- Immunocytochemistry: Human |Mouse | Rat
- Immunohistochemistry: Mouse | Rat | Zebra Finch
- Immunoprecipitation: Mouse | Rat
- PLA: Mouse
- Western Blot: Human | Mouse | Rat
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 38066086 | 1:1000 | Radulovic, T, et al. 2023. Serine 937 phosphorylation enhances KCC2 activity and strengthens synaptic inhibition. Scientific Reports, 21660. |
| 37625593 | 1:1000 | Becker, L, et al. 2023. Both chloride binding sites are required for KCC2 mediated-transport. The Journal of biological chemistry, 105190. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 33539918 | 1:500-1:1000 | Kontou, G., et al. 2021. KCC2 is required for the survival of mature neurons but not for their development. . Journal of Biological Chemistry, 100364. |
| 33192291 | not listed | Smalley, J.L., et al. 2020. Isolation and characterization of multi-protein complexes enriched in the K-Cl Co-transporter 2 from brain plasma membranes. . Frontiers in Molecular Neuroscience, 563091. |
| 30201606 | 1:1000 | Cordshagen, A., et al. 2018. Phosphoregulation of the intracellular termini of K+-Cl− cotransporter 2 (KCC2) enables flexible control of its activity. . Journal of Biological Chemistry, 16984-16993. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 32413057 | 1:1000 | Zhang, J., et al. 2020. Staurosporine and NEM mainly impair WNK-SPAK/OSR1 mediated phosphorylation of KCC2 and NKCC1. . PloS one, e0232967. |
| 22414695 | 1:500 | Döding, A., et al. 2012. KCC2 transport activity requires the highly conserved L₆₇₅ in the C-terminal β1 strand.. Biochemical and Biophysical Research Communications, 492-497. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 28054918 | not listed | Chen, M., et al. 2017. APP modulates KCC2 expression and function in hippocampal GABAergic inhibition.. Elife, e20142. |
| 24639001 | 1:300 | Markkanen, M., et al. 2014. Distribution of neuronal KCC2a and KCC2b isoforms in mouse CNS.. Journal of Comparative Neurology, 1897-1914. |
| 22674255 | 1:200 | Sun, Y.G., et al. 2012. GABAergic synaptic transmission triggers action potentials in thalamic reticular nucleus neurons.. Journal of Neuroscience, 7782-7790. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 31122143 | 1:40 | Lizhnyak, P.N., et al. 2019. Traumatic brain injury temporal proteome guides KCC2-targeted therapy. . Journal of neurotrauma, 3092-3102. |
| 30055019 | 1:500 | Zhang, H., et al. 2018. Uncovering a critical period of synaptic imbalance during postnatal development of the rat visual cortex: role of brain‐derived neurotrophic factor. . The Journal of Physiology, 4511-4536. |
| 22441038 | 1:500 | Liu, Q., et al. 2012. Postnatal development of Na(+)-K(+)-2Cl(-) co-transporter 1 and K(+)-Cl(-) co-transporter 2 immunoreactivity in multiple brain stem respiratory nuclei of the rat.. Neuroscience, 1-20. |
| 19674083 | not listed | Bragin, D.E., et al. 2009. Development of epileptiform excitability in the deep entorhinal cortex after status epilepticus.. European Journal of Neuroscience, 611-624. |
| 19001126 | not listed | Hedstrom, K.L., et al. 2008. AnkyrinG is required for maintenance of the axon initial segment and neuronal polarity.. The Journal of Biology, 635-640. |
| Immunohistochemistry: Zebra Finch | ||
| PMID | Dilution | Publication |
| 29218745 | 1:500-1:1500 | Vaaga, C.E., et al. 2018. Expression of the potassium‐chloride co‐transporter, KCC2, within the avian song system. . Journal of Comparative Neurology, 944-956. |
| Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 33192291 | not listed | Smalley, J.L., et al. 2020. Isolation and characterization of multi-protein complexes enriched in the K-Cl Co-transporter 2 from brain plasma membranes. . Frontiers in Molecular Neuroscience, 563091. |
| 31615901 | not listed | Watanabe, M., et al. 2019. Developmentally regulated KCC2 phosphorylation is essential for dynamic GABA-mediated inhibition and survival. . Science signaling, eaaw9315. |
| 29028184 | not listed | Mahadevan, V., et al. 2017. Native KCC2 interactome reveals PACSIN1 as a critical regulator of synaptic inhibition. . Elife, e28270. |
| 24910435 | 5-10 ug | Mahadevan, V., et al. 2014. Kainate receptors coexist in a functional complex with KCC2 and regulate chloride homeostasis in hippocampal neurons. . Cell reports, 1762-1770. |
| 20529123 | 1:100 | Horn, Z., et al. 2010. Premature expression of KCC2 in embryonic mice perturbs neural development by an ion transport-independent mechanism.. European Journal of Neuroscience, 2142-2155. |
| Immunoprecipitation: Rat | ||
| PMID | Dilution | Publication |
| 29216042 | not listed | Cardarelli, R.A., et al. 2017. The small molecule CLP257 does not modify activity of the K+–Cl− co-transporter KCC2 but does potentiate GABAA receptor activity. . Nature medicine, 1394-1396. |
| 29092909 | not listed | Conway, L.C., et al. 2017. N-Ethylmaleimide increases KCC2 cotransporter activity by modulating transporter phosphorylation. . Journal of Biological Chemistry, 21253-21263. |
| 24097188 | 5 ug | Gagnon, M., et al. 2013. Chloride extrusion enhancers as novel therapeutics for neurological diseases.. Nature Medicine, 1524-1528. |
| PLA: Mouse | ||
| PMID | Dilution | Publication |
| 28054918 | not listed | Chen, M., et al. 2017. APP modulates KCC2 expression and function in hippocampal GABAergic inhibition.. Elife, e20142. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 35678782 | 1ug/ml | Küry, S., et al. 2022. Rare Pathogenic Variants in WNK3 Cause X-Linked Intellectual Disability. Genetics in Medicine, . |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 36889319 | 1:500 | Jarvis, R., et al. 2023. Direct Activation of KCC2 Arrests Benzodiazepine Refractory Status Epilepticus and Limits the Subsequent Neuronal Injury in Mice. Cell Reports Medicine, 100957. |
| 33539918 | 1:1000 | Kontou, G., et al. 2021. KCC2 is required for the survival of mature neurons but not for their development. . Journal of Biological Chemistry, 100364. |
| 31615901 | not listed | Watanabe, M., et al. 2019. Developmentally regulated KCC2 phosphorylation is essential for dynamic GABA-mediated inhibition and survival. . Science signaling, eaaw9315. |
| 31615899 | not listed | Pisella, L.I., et al. 2019. Impaired regulation of KCC2 phosphorylation leads to neuronal network dysfunction and neurodevelopmental pathology. . Science signaling, eaay0300. |
| 28054918 | not listed | Chen, M., et al. 2017. APP modulates KCC2 expression and function in hippocampal GABAergic inhibition. . Elife, e20142. |
| 26441539 | not listed | Mahadevan, V., et al. 2015. Neto2-null mice have impaired GABAergic inhibition and are susceptible to seizures.. Frontiers in Cellular Neuroscience, 368. |
| 25066727 | 1:1000 | Campbell, S.L., et al. 2015. GABAergic disinhibition and impaired KCC2 cotransporter activity underlie tumor-associated epilepsy.. Glia, 23-36. |
| 24910435 | 1:500 | Mahadevan, V., et al. 2014. Kainate receptors coexist in a functional complex with KCC2 and regulate chloride homeostasis in hippocampal neurons. . Cell reports, 1762-1770. |
| 24403144 | 1:500 | He, Q., et al. 2014. The developmental switch in GABA polarity is delayed in fragile X mice.. Journal of Neuroscience, 446-450. |
| 28496181 | not listed | Kursan, S., et al. 2017. The neuronal K+ Cl− co-transporter 2 (Slc12a5) modulates insulin secretion. . Scientific reports, 1-14. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 31911626 | not listed | Zhang, J., et al. 2020. Modulation of brain cation-Cl− cotransport via the SPAK kinase inhibitor ZT-1a. . Nature communications, 1-17. |
| 31122143 | 1:300 | Lizhnyak, P.N., et al. 2019. Traumatic brain injury temporal proteome guides KCC2-targeted therapy. . Journal of neurotrauma, 3092-3102. |
| 28235805 | 1:1000 | Pressey, J.C., et al. 2017. A kainate receptor subunit promotes the recycling of the neuron-specific K+-Cl− co-transporter KCC2 in hippocampal neurons. . Journal of Biological Chemistry, 6190-6201. |
| 22044736 | 1:250 | Reid, A.Y., et al. 2011. A prolonged experimental febrile seizure results in motor map reorganization in adulthood.. Neurobiology of Disease, 692-700. |
| 19674083 | 1:1000 | Bragin, D.E., et al. 2009. Development of epileptiform excitability in the deep entorhinal cortex after status epilepticus.. European Journal of Neuroscience, 611-624. |
| 17693402 | 1:1000 | Lee, H.H., et al. 2007. Direct protein kinase C-dependent phosphorylation regulates the cell surface stability and activity of the potassium chloride cotransporter KCC2.. The Journal of Biological Chemistry, 29777-29784. |