Anti-Kv1.1 K+ Channel (External) Antibody (K36/15)

Name: Anti-Kv1.1 K+ Channel (External) Antibody
Brand: NeuroMab
SKU: 75-105
Price: 374.0 USD
Availability: InStock

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Our Anti-Kv1.1 K+ channel (external) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K36/15. It is KO validated, detects mouse and rat Kv1.1 K+ channel (external), and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot versus crude brain membranes from adult mouse (MBM), rat (RBM) and human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with K36/15 (left) or N52A/42 (right) TC supe.

Immunofluorescence staining of hippocampus from adult Kv1.1 wildtype (left) or knock-out (right) mouse with K36/15 (red) and K13/31 (green, Kv1.4).

Representative confocal micrographs of paranode (CASPR, brace; red) pairs with interspersed node of Ranvier (bracket), demarcated by juxtaparanodes (KV1.1; white) in brain from with standard-housed (SH) or environmental enrichment (EE) mice. Image from publication CC-BY-4.0. PMID:36217300

Low magnification image of mouse cerebellar cortex folium section showing KV1.1 (cat. 75-105, 1:1000) immunofluorescence . Right. High magnification confocal image showing concentrated KV1.1 immunofluorescence (green) localized at the interface of Purkinje and granule cell layers. Right lower. Same image showing co-immunofluorescence labelling for the Purkinje cell marker calbindin (magenta). Image from publication CC-BY-4.0. PMID: 37408217

Immunoblot against crude brain membranes from adult rat (RBM) and Kv1.1 wild-type (WT) or knock-out (KO) mouse probed with K36/15 (left) or N52A/42 (right) TC supe.

Representative confocal sections of lamina adjacent to the Purkinje cell layer from Kcna1V408A/+ and control cerebellar cortex, labelled for KV1.1 (Cat.75-105, 1:1000; yellow) and PSD-95 (centre, magenta). Right, merged image. Image from publication CC-BY-4.0. PMID: 37408217

Immunolabeling of postnatal 14 mouse CA1 (Axonal Initial Segment, Interneuron) specifically labeling extracellular Kv1.1 (Cat no 75-105, 1:200, green) and Ankyrin G (red). DNA labeled with DAPI. Mice transcardially perfused with cold 4% PFA in PBS, brain removed and postfixed overnight. 50 uM slices as floating sections, antigen retrieval performed with sodium citrate at 80 C for 30 min before immunolabeling. Images from: J. Ramirez-Franco and O. El Far, Aix-Marseille Université and INSERM.

Immunoblot versus crude brain membranes from adult mouse (MBM), rat (RBM) and human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with K36/15 (left) or N52A/42 (right) TC supe.

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-105

Volume: 100 µL

Variant

Price:

$374.00

Quantity:

Product Details

Target

Kv1.1 K+ channel (external)

Target Description

Potassium Channel Kv1.1or Kcna1 is a member of the Shaker potassium channel family. Kv1.1 is expressed in the brain, CNS and in the kidney. Potassium channels form homotetrameric and heterotetrameric channels in the membrane with various other related proteins, including KCNA2, KCNA4, KCNA5, KCNA6, KCNA7. Kv1.1 is involved with the regulation of cellular functions and the control of neuronal excitability. Mutations in Kv1.1 are associated with Episodic ataxia type 1 and epilepsy.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

K36/15

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Kcna1

Molecular Weight

56 kDa (could be 65-85 kDa depending on glycosylation)

Antigen

Synthetic peptide amino acids 191-208 (ELKDDKDFTGTIHRIDNTC, extracellular domain) of rat Kv1.1 (accession number P10499)

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2128566

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.33

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Potassium voltage-gated channel subfamily A member 1 (RBKI) (RCK1) (Voltage-gated potassium channel subunit Kv1.1)

UniProt Details

UniProt (Human): Q09470
UniProt (Immunogen Species): P10499

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse | Rat
Immunohistochemistry: Mouse | Rat
Immunoprecipitation: Mouse
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Human
PMID	Dilution	Publication
28115470	not listed	Lang, B., et al. 2017. Intracellular and non-neuronal targets of voltage-gated potassium channel complex antibodies. Journal of Neurology, Neurosurgery & Psychiatry, 88(4), pp.353-361.. Journal of Neurology, Neurosurgery & Psychiatry, 353-361.
23774215	not listed	Lallet-Daher, H., et al. 2013. Potassium channel KCNA1 modulates oncogene-induced senescence and transformation.. Cancer Research, 5253-5265.
20663977	1:100	Irani, S.R., et al. 2010. Antibodies to Kv1 potassium channel-complex proteins leucine-rich, glioma inactivated 1 protein and contactin-associated protein-2 in limbic encephalitis, Morvan''s syndrome and acquired neuromyotonia.. Brain, 2734-2748.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
28331203	1:100	Rama, S., et al. 2017. The role of axonal Kv1 channels in CA3 pyramidal cell excitability. . Scientific reports, 1-12.
21224222	1:2000	Karcz, A., et al. 2011. Low‐voltage activated Kv1. 1 subunits are crucial for the processing of sound source location in the lateral superior olive in mice. . The Journal of physiology, 1143-1157.

Immunocytochemistry: Rat
PMID	Dilution	Publication
33185190	1:100	Sanders, S.S., et al. 2020. The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment. Elife, e56058.
33168717	not listed	Cho, I.H., et al. 2020. The potassium channel subunit Kvβ1 serves as a major control point for synaptic facilitation. PNAS:USA, 29937-29947.
25447742	1:1000	Hoppa, M.B., et al. 2014. Control and plasticity of the presynaptic action potential waveform at small CNS nerve terminals.. Neuron, 778-789.
23836929	1:1000	Sosanya, N.M., et al. 2013. Degradation of high affinity HuD targets releases Kv1.1 mRNA from miR-129 repression by mTORC1.. The Journal of Biology, 53-69.
21357749	not listed	Vacher, H., et al. 2011. Cdk-mediated phosphorylation of the Kvβ2 auxiliary subunit regulates Kv1 channel axonal targeting.. The Journal of Biology, 813-24.
17185748	not listed	Vacher, H., et al. 2007. Regulation of Kv1 channel trafficking by the mamba snake neurotoxin dendrotoxin K.. The FASEB Journal, 906-914.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
38663634	not listed	Ramirez-Franco, J, et al. 2024. The downregulation of Kv1 channels in Lgi1-/-mice is accompanied by a profound modification of its interactome and a parallel decrease in Kv2 channels. Neurobiology of Disease, 106513.
38194969	not listed	Miyazaki, Y, et al. 2024. Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity. Cell Reports, 113634.
37408217	1:1000	Martin, H, et al. 2023. Basket to Purkinje Cell Inhibitory Ephaptic Coupling Is Abolished in Episodic Ataxia Type 1. Cells, 1382.
37283058	1:750	Dustin, E, et al. 2023. Adult-onset Depletion of Sulfatide Leads to Axonal Degeneration with Relative Myelin Sparing. Glia, 2285-2303.
36849716	1:100	Hu, A., et al. 2023. Projection-Specific Heterogeneity of the Axon Initial Segment of Pyramidal Neurons in the Prelimbic Cortex. Neuroscience Bulletin, .
36640913	1:500	Otuyemi, B., et al. 2023. Domain and Cell Type-specific Immunolocalisation of Voltage-gated Potassium Channels in the Mouse Striatum. Journal of Chemical Neuroanatomy, 102233.
36217300	1:200	Nicholson, M., et al. 2022. Remodelling of Myelinated Axons and Oligodendrocyte Differentiation is Stimulated by Environmental Enrichment in the Young Adult Brain. The European Journal of Neuroscience, 6099-6114.
35661858	1:500	Nascimento, A.I., et al. 2022. Sensory neurons have an axon initial segment that initiates spontaneous activity in neuropathic pain. Brain, 1632-1640.
31761670	1:200	Klingseisen, A., et al. 2019. Oligodendrocyte neurofascin independently regulates both myelin targeting and sheath growth in the CNS. . Developmental cell, 730-744.
31248728	1:250	Zheng, Y., et al. 2019. Deep sequencing of somatosensory neurons reveals molecular determinants of intrinsic physiological properties. . Neuron, 598-616.
29476105	1:200	Nguyen, L.H., et al. 2018. mTOR-dependent alterations of Kv1.1 subunit expression in the neuronal subset-specific Pten knockout mouse model of cortical dysplasia with epilepsy. Scientific Reports, 3568.
29476105	1:200	Nguyen, L.H., et al. 2018. mTOR-dependent alterations of Kv1.1 subunit expression in the neuronal subset-specific Pten knockout mouse model of cortical dysplasia with epilepsy. Scientific Reports, 3568.
28134616	not listed	Amor, V., et al. 2017. The paranodal cytoskeleton clusters Na+ channels at nodes of Ranvier. . Elife, e21392.
25290690	not listed	Jones, K.S., et al. 2014. Neonatal NMDA receptor blockade disrupts spike timing and glutamatergic synapses in fast spiking interneurons in a NMDA receptor hypofunction model of schizophrenia.. PLoS One, e109303.
24719087	1:100	Desmazieres, A., et al. 2014. Differential stability of PNS and CNS nodal complexes when neuronal neurofascin is lost.. Journal of Neuroscience, 5083-5088.
24200924	1:40	Liu, Q., et al. 2014. Heterogeneous intrinsic excitability of murine spiral ganglion neurons is determined by Kv1 and HCN channels.. Neuroscience, 96-110.
22612819	not listed	Menegola, M., et al. 2012. The importance of immunohistochemical analyses in evaluating the phenotype of Kv channel knockout mice.. Epilepsia, 142-149.
22473710	1:50	Irani, S.R., et al. 2012. Morvan syndrome: clinical and serological observations in 29 cases.. Annals of Neurology, 241-255.
21958379	not listed	Duflocq, A., et al. 2011. Characterization of the axon initial segment (AIS) of motor neurons and identification of a para-AIS and a juxtapara-AIS, organized by protein 4.1B.. BMC Biology, 1-19.
21220016	not listed	Giacomini, C., et al. 2011. Both Schwann cell and axonal defects cause motor peripheral neuropathy in Ebf2-/- mice.. Neurobiology of Disease, 73-84.
19118165	1:1000	Lorincz, A., et al. 2008. Cell-type-dependent molecular composition of the axon initial segment. . Journal of Neuroscience, 14329-14340.
19091962	1:1000	Miller, M.N., et al. 2008. Region-specific spike-frequency acceleration in layer 5 pyramidal neurons mediated by Kv1 subunits.. Journal of Neuroscience, 13716-13726.
18466749	10ug/ml	Goldberg, E.M., et al. 2008. K+ channels at the axon initial segment dampen near-threshold excitability of neocortical fast-spiking GABAergic interneurons.. Neuron, 387-400.

Immunohistochemistry: Rat
PMID	Dilution	Publication
36050224	1:500	Sharma, K., et al. 2022. Low-Voltage Activating K+ Channels in Cochlear Afferent Nerve Fiber Dendrites. Experimental Neurobiology, 243-259.
34799447	1:100	Zbili, M., et al. 2021. Homeostatic regulation of axonal Kv1. 1 channels accounts for both synaptic and intrinsic modifications in the hippocampal CA3 circuit. PNAS:USA, .
32345673	1:300	Housley, S.N., et al. 2020. Cancer exacerbates chemotherapy-induced sensory neuropathy. . Cancer research, 2940-2955.
28331203	1:50	Rama, S., et al. 2017. The role of axonal Kv1 channels in CA3 pyramidal cell excitability. . Scientific reports, 1-12.
24723263	1:100	Dufour, M.A., et al. 2014. Somatodendritic ion channel expression in substantia nigra pars compacta dopaminergic neurons across postnatal development.. Journal of Neuroscience Research, 981-999.
24606584	1:250	Kirizs, T., et al. 2014. Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells.. European Journal of Neuroscience, 1711-1783.
23439123	1:50	Campanac, E., et al. 2013. Enhanced intrinsic excitability in basket cells maintains excitatory-inhibitory balance in hippocampal circuits.. Neuron, 712-722.
19118165	1:1000	Lorincz, A., et al. 2008. Cell-type-dependent molecular composition of the axon initial segment. . Journal of Neuroscience, 14329-14340.

Immunoprecipitation: Mouse
PMID	Dilution	Publication
22067099	15ug/ml	Bildl, W., et al. 2012. Extending the dynamic range of label-free mass spectrometric quantification of affinity purifications.. Molecular & Cellular Proteomics, .
22158511	10ul	Li, K.K., et al. 2011. Neuregulin 1 regulates excitability of fast-spiking neurons through Kv1.1 and acts in epilepsy.. Nature Neuroscience, 267-273.

Western Blot: Human
PMID	Dilution	Publication
23774215	not listed	Lallet-Daher, H., et al. 2013. Potassium channel KCNA1 modulates oncogene-induced senescence and transformation.. Cancer Research, 5253-5265.
23734863	not listed	Zhao, J., et al. 2013. Spinocerebellar ataxia-13 Kv3.3 potassium channels: arginine-to-histidine mutations affect both functional and protein expression on the cell surface.. Biochemical Journal, 259-265.
22609616	1:1000	Zhu, J., et al. 2012. Characterization of the Kv1.1 I262T and S342I mutations associated with episodic ataxia 1 with distinct phenotypes.. Archives of Biochemistry and Biophysics, 99-105.

Western Blot: Mouse
PMID	Dilution	Publication
38663634	not listed	Ramirez-Franco, J, et al. 2024. The downregulation of Kv1 channels in Lgi1-/-mice is accompanied by a profound modification of its interactome and a parallel decrease in Kv2 channels. Neurobiology of Disease, 106513.
38194969	not listed	Miyazaki, Y, et al. 2024. Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity. Cell Reports, 113634.
25707359	not listed	Chen, N., et al. 2015. Interaction proteomics of canonical Caspr2 (CNTNAP2) reveals the presence of two Caspr2 isoforms with overlapping interactomes.. Biochimica et Biophysica Acta, 827-833.
23945775	not listed	Kyle, B.D., et al. 2013. The large conductance, calcium-activated K+ (BK) channel is regulated by cysteine string protein.. Scientific Reports, 2447.
22449950	not listed	Bangratz, M., et al. 2012. A mouse model of Schwartz-Jampel syndrome reveals myelinating Schwann cell dysfunction with persistent axonal depolarization in vitro and distal peripheral nerve hyperexcitability when perlecan is lacking.. The American Journal of Pathology, 2040-2055.
22067099	1:10,000	Bildl, W., et al. 2012. Extending the dynamic range of label-free mass spectrometric quantification of affinity purifications.. Molecular & Cellular Proteomics, .

Western Blot: Rat
PMID	Dilution	Publication
33185190	1:500	Sanders, S.S., et al. 2020. The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment. Elife, e56058.
32437528	not listed	Ramberger, M., et al. 2020. Distinctive binding properties of human monoclonal LGI1 autoantibodies determine pathogenic mechanisms. Brain, 1731-1745.
28673977	not listed	Seagar, M., et al. 2017. LGI1 tunes intrinsic excitability by regulating the density of axonal Kv1 channels. Proceedings of the National Academy of Sciences, 114(29), pp.7719-7724.. Proceedings of the National Academy of Sciences, 7719-7724.
25270294	1 : 500	Sosanya, N.M., et al. 2015. Rapamycin reveals an mTOR-independent repression of Kv1.1 expression during epileptogenesis.. Neurobiology of Disease, 96-105.
17185748	not listed	Vacher, H., et al. 2007. Regulation of Kv1 channel trafficking by the mamba snake neurotoxin dendrotoxin K.. The FASEB Journal, 906-914.

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Anti-Kv1.1 K+ Channel (External) Antibody (K36/15)

SKU: 75-105

Product Details

Product Specific References for Applications and Species

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