Anti-Neuroligin-1 Antibody (N97A/31)
Our Anti-Neuroligin-1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N97A/31. It is KO validated, detects mouse and rat Neuroligin-1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
![Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N97A/31 (left) or N52A/42 (right) TC supe.](http://www.antibodiesinc.com/cdn/shop/files/73-160-n97a-31-wb-2_{width}x.jpg?v=1704392947)
Adult rat brain membrane immunoblot.
![Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N97A/31 (left) or N52A/42 (right) TC supe.](http://www.antibodiesinc.com/cdn/shop/files/73-160-n97a-31-wb-2_1024x.jpg?v=1704392947)
Chicken, Human, Mouse, Rat
ICC, IHC, WB
Mouse
KO Validated
Product Specific References for Applications and Species
- Immunocytochemistry: Human | Mouse
- Immunohistochemistry: Chicken | Human | Mouse
- Western Blot: Chicken | Human | Mouse | Rat
- Additional Publications: Unspecified
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 32434929 | not listed | Kim, H., et al. 2020. Calsyntenin-3 interacts with both α-and β-neurexins in the regulation of excitatory synaptic innervation in specific Schaffer collateral pathways. Journal of Biological Chemistry, 9244-9262. |
| 22355283 | not listed | Yamagata, M., et al. 2012. Transgenic strategy for identifying synaptic connections in mice by fluorescence complementation (GRASP).. Frontiers in Molecular Neuroscience, 18. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 36007521 | 1:1000 | Nozawa, K., et al. 2022. In vivo nanoscopic landscape of neurexin ligands underlying anterograde synapse specification. Neuron, 3168-3185. |
| 33083769 | 20ug/ml | Verstraelen, P., et al. 2020. Systematic quantification of synapses in primary neuronal culture. iScience, 101542. |
| 29760652 | not listed | Yamagata, M., et al. 2018. Cadherins Interact With Synaptic Organizers to Promote Synaptic Differentiation.. Frontiers in Molecular Neuroscience, 142. |
| Immunohistochemistry: Chicken | ||
| PMID | Dilution | Publication |
| 21031560 | 1:500 | Wahlin KJ, et al. 2010. Alternative splicing of neuroligin and its protein distribution in the outer plexiform layer of the chicken retina.. Journal of Comparative Neurology, 4938-62. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 36056393 | 1:100 | Pergolizzi, M., et al. 2022. The Neuronal Protein Neuroligin 1 Promotes Colorectal Cancer Progression by Modulating the APC/β-catenin Pathway. Journal of Experimental & Clinical Cancer Research, 266. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 25352748 | 1:400 | de Andrade, G.B., et al. 2014. Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina.. Molecular Vision, 1422-33. |
| Western Blot: Chicken | ||
| PMID | Dilution | Publication |
| 21031560 | 1:500 | Wahlin KJ, et al. 2010. Alternative splicing of neuroligin and its protein distribution in the outer plexiform layer of the chicken retina.. Journal of Comparative Neurology, 4938-62. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 32434929 | not listed | Kim, H., et al. 2020. Calsyntenin-3 interacts with both α-and β-neurexins in the regulation of excitatory synaptic innervation in specific Schaffer collateral pathways. Journal of Biological Chemistry, 9244-9262. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 38472649 | 1:1000 | Campos, J., et al. 2024. Liprin-α proteins are master regulators of human presynapse assembly. Nature Neuroscience, . |
| 37308047 | 1:800 | Hernandez, A, et al. 2023. Striatal Synaptic Changes and Behavior in Adult Mouse Upon Prenatal Exposure to Valproic Acid. Brain Research, 148461. |
| 37260747 | 1:1000 | Arias-Aragón, F, et al. 2023. A Neuroligin-1 Mutation Associated with Alzheimer's Disease Produces Memory and Age-dependent Impairments in Hippocampal Plasticity. iScience, 106868. |
| 37078449 | 1:3000 | Yokokawa, T., et al. 2023. Altered Expression of Synaptic Proteins and Adhesion Molecules in the Hippocampus and Cortex Following the Onset of Diabetes in Nonobese Diabetic Mice. Physiological Reports, e15673. |
| 33083769 | 1ug/ml | Verstraelen, P., et al. 2020. Systematic quantification of synapses in primary neuronal culture. iScience, 101542. |
| 28864826 | 1:300 | Tanabe, Y., et al. 2017. IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2α. Nature communications, 408. |
| 27005990 | 1:10000 | Heise, C., et al. 2017. eEF2K/eEF2 pathway controls the excitation/inhibition balance and susceptibility to epileptic seizures. Cerebral Cortex, 27(3), pp.2226-2248.. Cerebral Cortex, 2226-2248. |
| 26859820 | 1:2000 | Kim, J.Y.V., et al. 2016. Neuroligin 2 regulates spinal GABAergic plasticity in hyperalgesic priming, a model of the transition from acute to chronic pain. Pain, 1314-1324. |
| 26776509 | not listed | Um, J.W., et al. 2016. LRRTM3 regulates excitatory synapse development through alternative splicing and neurexin binding. Cell Reports, 808-822. |
| 26094033 | not listed | Zou, D., et al. 2015. A critical role of RBM8a in proliferation and differentiation of embryonic neural progenitors.. Neural Development, 18. |
| 24795561 | 1:1000 | Lugo, J.N., et al. 2014. Deletion of PTEN produces autism-like behavioral deficits and alterations in synaptic proteins.. Frontiers in Molecular Neuroscience, 27. |
| 24448406 | not listed | Li Q, et al. 2014. The splicing regulator PTBP2 controls a program of embryonic splicing required for neuronal maturation.. Elife, e01201. |
| 23719805 | 1 : 1000 | Hoy, J.L., et al. 2013. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions.. Journal of Neuroscience, 9364-9384. |
| 21145003 | 1:1000 | Robbins EM, et al. 2010. SynCAM 1 adhesion dynamically regulates synapse number and impacts plasticity and learning.. Neuron, 894-906. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 32801157 | not listed | Culotta, L., et al. 2020. SULT4A1 modulates synaptic development and function by promoting the formation of PSD-95/NMDAR complex. Journal of Neuroscience, 7013-7026. |
| 28362857 | 3.3ug/ml | Burch, A., et al. 2017. A novel synaptic junction preparation for the identification and characterization of cleft proteins.. PLoS One, e0174895. |
| 27869801 | 1:1000 | Vieweg, S., et al. 2017. Direct dorsal hippocampal–prelimbic cortex connections strengthen fear memories. Nature neuroscience, 20(1), pp.52-61.. Nature neuroscience, 52-61. |
| 27677849 | 1:200 | Hanus, C., et al. 2016. Unconventional secretory processing diversifies neuronal ion channel properties. Elife, e20909. |
| 27002143 | not listed | Um, J.W., et al. 2016. IQ Motif and SEC7 Domain-containing Protein 3 (IQSEC3) Interacts with Gephyrin to Promote Inhibitory Synapse Formation.. The Journal of Biological Chemistry, 10119-10130. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 30602588 | Binda, C.S., et al. 2019. Sorting nexin 27 rescues neuroligin 2 from lysosomal degradation to control inhibitory synapse number. The Biochemical Journal, 293-306. | |
| 30046996 | Nozawa, K., et al. 2018. Cellular and Subcellular Localization of Endogenous Neuroligin-1 in the Cerebellum. Cerebellum, 709-721. | |