Anti-Pan-Gamma-Protocadherin-A Antibody (N144/32)

Name: Anti-Pan-Gamma-Protocadherin-A Antibody
Brand: NeuroMab
SKU: 75-178
Price: 319.0 USD
Availability: InStock

8 Citations

Our Anti-Pan-Gamma-protocadherin-A mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N144/32. It is KO validated, detects mouse Pan-Gamma-protocadherin-A, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Immunoblot versus brain samples from WT and Gamma- protocadherin KO mice. Data courtesy of Joshua Weiner, University of Iowa.

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Gamma protocadherin-A3 and -A4 and untagged Kv2.1 plasmids and probed with N144/32 (left) and N159/5 (right) TC supe.

Immunoblot versus brain samples from WT and Gamma- protocadherin KO mice. Data courtesy of Joshua Weiner, University of Iowa.

Species Reactivity

Mouse

Applications

ICC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-178

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Pan-Gamma-protocadherin-A

Target Description

Cadherins are calcium-dependent cell-cell adhesion molecules, and protocadherins constitute a subfamily of non-classic cadherins. Members of the protocadherin gamma subfamily are encoded by 22 tandemly arranged genes within the PCDHG gene cluster on chromosome 5q31. The 22 PCDHG genes function as 'variable' exons that are individually spliced to a downstream constant region to form distinct PCDHG transcripts. The variable PCDHG exons encode the extracellular and transmembrane domains of the protocadherin protein, and the common region encodes the intracellular domain. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N144/32

Isotype

IgG2b

Applications

ICC, WB

Host Species

Mouse

Gene Name

Pcdhga3

Molecular Weight

100 kDa

Antigen

Fusion protein corresponding to amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma-protocadherin-A3 (accession number Q91XY5) produced recombinantly in E. Coli

Species Reactivity

Mouse

Antibody Registry ID

AB_2159447

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.106

Conjugate

Unconjugated

Specificity

Cross-reacts with all Gamma protocadherin-A, -B and -C proteins

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Protocadherin gamma A3 (Protocadherin gamma subfamily A, 3)

UniProt Details

UniProt (Human): N/A
UniProt (Immunogen Species): Q91XY5

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Rat

Immunoprecipitation: Mouse

Western Blot: Human | Mouse

Immunocytochemistry: Rat
PMID	Dilution	Publication
31609469	1:15	Miralles, C.P., et al. 2020. Expression of protocadherin-γC4 protein in the rat brain. Journal of Comparative Neurology, 840-864.

Immunohistochemistry: Rat
PMID	Dilution	Publication
20589908	1:15	Li, Y., et al. 2010. Synaptic and nonsynaptic localization of protocadherin-gammaC5 in the rat brain.. Journal of Comparative Neurology, 3439-3462.

Immunoprecipitation: Mouse
PMID	Dilution	Publication
22884324	not listed	Chen, W.V., et al. 2012. Functional significance of isoform diversification in the protocadherin gamma gene cluster. Neuron, 402-409.

Western Blot: Human
PMID	Dilution	Publication
31943018	1:1000	Ponroy Bally, B., et al. 2020. Human iPSC-derived Down syndrome astrocytes display genome-wide perturbations in gene expression, an altered adhesion profile, and increased cellular dynamics. . Human Molecular Genetics, 785-802.
31940498	not listed	Joutsen, J., et al. 2020. Heat Shock Factor 2 Protects against Proteotoxicity by Maintaining Cell-Cell Adhesion. Cell Reports, 583-597.

Western Blot: Mouse
PMID	Dilution	Publication
38837880	1:500	Hanes, C.M., et al. 2024. A C-terminal motif containing a PKC phosphorylation site regulates γ-Protocadherin-mediated dendrite arborization in the cerebral cortex in vivo. Developmental Neurobiology, 217-235.
31877124	1:500-1:1000	Garrett, A.M., et al. 2019. CRISPR/Cas9 interrogation of the mouse Pcdhg gene cluster reveals a crucial isoform-specific role for Pcdhgc4. . PLoS genetics, e1008554.
22884324	not listed	Chen, W.V., et al. 2012. Functional significance of isoform diversification in the protocadherin gamma gene cluster. Neuron, 402-409.
22092001	not listed	Lobas, M.A., et al. 2012. Molecular heterogeneity in the choroid plexus epithelium: the 22-member γ-protocadherin family is differentially expressed, apically localized, and implicated in CSF regulation.. Journal of Neurochemistry, 913-927.

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Anti-Pan-Gamma-Protocadherin-A Antibody (N144/32)

SKU: 75-178

Product Details

Product Specific References for Applications and Species

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