Anti-QKI-5 Antibody (N195A/16)

Name: Anti-QKI-5 Antibody
Brand: NeuroMab
SKU: 75-232
Price: 319.0 USD
Availability: InStock

Our Anti-QKI-5 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N195A/16. It detects human, mouse, and rat QKI-5, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged QKI-5, QKI- 6, QKI-7 and QKI-7b and untagged Kv2.1 plasmids and probed with N195A/16 TC supe (left) and mouse anti- Flag (right).

Western blot of protein extracted from UD hESCs, DE cells, and CM cells probed with antibodies for QKI5 (magenta, top panel) and panQKI (green), QKI6 (magenta, middle panel) and panQKI (green), or QKI7 (magenta, bottom panel; ND denotes not detectable) and panQKI (green). Image from publication CC-BY-4.0. PMID: 35544276

Adult rat brain membrane immunoblot.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-232

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

QKI-5

Target Description

QKI5 is a member of the Signal Transduction and Activation of RNA proteins (STAR proteins) family. QKI5, QKI6 QKI7 and QKI7b are alternatively spliced mRNAs from the QKI1 gene. QKI5 is a RNA-binding protein. QKI5 has roles in the regulation of splicing, export and stability of mRNA. It is involved in myelinization. It is a nuclear isoform and contains a C-terminal nuclear localization signal. Diseases associated with this gene include Angiocentric Glioma, 6Q Terminal Deletion Syndrome .and Schizophrenia.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N195A/16

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

Qki Qk QKI HKQ Qk1 Qka1

Molecular Weight

40 kDa (and others)

Antigen

Synthetic peptide amino acids 315-331 (ATKVRRHDMRVHPYQRI, with nuclear localization signal underlined) of human QKI-5 (accession number Q96PU)

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10674117

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with QKI-6, QKI-7 or QKI-7bCross-reacts with other STAR proteins containing similar nuclear localization signals

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Protein quaking (Hqk) (HqkI)

UniProt Details

UniProt (Human): Q96PU8
UniProt (Immunogen Species): Q96PU8

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Human
Western Blot: Human | Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
27454326	1:100	Bin, J.M., et al. 2016. The oligodendrocyte-specific antibody ''CC1'' binds Quaking 7.. Journal of Neurochemistry, 181-186.

Immunohistochemistry: Human
PMID	Dilution	Publication
27029405	10ug/ml	De Bruin, R.G., et al. 2016. Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression. Nature Communications, 10846.

Western Blot: Human
PMID	Dilution	Publication
35544276	not listed	Fagg, W.S., et al. 2022. Definition of germ layer cell lineage alternative splicing programs reveals a critical role for Quaking in specifying cardiac cell fate. Nucleic acids research, 5313-5334.
27454326	1:1000	Bin, J.M., et al. 2016. The oligodendrocyte-specific antibody ''CC1'' binds Quaking 7.. Journal of Neurochemistry, 181-186.

Western Blot: Mouse
PMID	Dilution	Publication
34968236	not listed	de Bruin, R.G., et al. 2020. Targeting the RNA-Binding Protein QKI in Myeloid Cells Ameliorates Macrophage-Induced Renal Interstitial Fibrosis. Targeting the RNA-Binding Protein QKI in Myeloid Cells Ameliorates Macrophage-Induced Renal Interstitial Fibrosis, 2.
23963726	not listed	van der Veer, E.P., et al. 2013. Quaking, an RNA-binding protein, is a critical regulator of vascular smooth muscle cell phenotype.. Circulation Research, 1065-1075.
20956316	not listed	Zhao, L., et al. 2010. Quaking I controls a unique cytoplasmic pathway that regulates alternative splicing of myelin-associated glycoprotein.. PNAS USA, 19061-19066.

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Anti-QKI-5 Antibody (N195A/16)

SKU: 75-232

Product Details

Product Specific References for Applications and Species

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