Anti-REEP1 Antibody (N345/51)

Name: Anti-REEP1 Antibody
Brand: NeuroMab
SKU: 75-313
Price: 319.0 USD
Availability: InStock

Our Anti-REEP1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N345/51. It detects mouse and rat REEP1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).

Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with Flag- tagged REEP1, REEP2 or untagged Kv2.1 plasmid and probed with N345/51 (left) and K89/34 (right) TC supe.

Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebellum [HBM(Cb)] or cerebral cortex [HBM(Cx)] and probed with N345/51 (left) or N52A/42 (right) TC supe.

Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-313

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

REEP1

Target Description

Receptor expression-enhancing protein 1, Receptor Accessory Protein 1 or REEP1 is encoded by the gene REEP1. The REEP protein family is made up of six REEP proteins 1-6. REEP1 is a mitochondrial protein that links ER tubules to the cytoskeleton and is involved in ER formation and remodeling. REEP1 is expressed in brain, spinal cord and testes. It is also expressed in olfactory sensory neurons and may play a role in the cell surface expression of odorant receptors. Diseases associated with this gene include Spastic Paraplegia distal hereditary motor neuropathy type V. Ref: Brain Res. 2014 January 30; 1545: 12–22. doi:10.1016/j.brainres.2013.12.008

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N345/51

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Reep1 D6Ertd253e C2orf23 SPG31

Molecular Weight

22 kDa

Antigen

Fusion protein amino acids 111-201 (KDRSYDALVHFGKRGLNVAATAAVMAASKGQGALSERLRSFSMQDLTTIRGDGAPAPSGPPPPGTGRSSGKHSQPKMSRSASESAGSSGTA, cytoplasmic C-terminus) of mouse REEP1 (accession number Q8BGH4) produced recombinantly in E. Coli

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2315913

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with REEP2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Receptor expression-enhancing protein 1 (Spastic paraplegia 31 protein)

UniProt Details

UniProt (Human): Q9H902
UniProt (Immunogen Species): Q8BGH4

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Western Blot: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
24355597	not listed	Hurt, C.M., et al. 2014. REEP1 and REEP2 proteins are preferentially expressed in neuronal and neuronal-like exocytotic tissues. Brain Research, 45648.

Western Blot: Mouse
PMID	Dilution	Publication
24355597	not listed	Hurt, C.M., et al. 2014. REEP1 and REEP2 proteins are preferentially expressed in neuronal and neuronal-like exocytotic tissues. Brain Research, 45648.

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Anti-REEP1 Antibody (N345/51)

SKU: 75-313

Product Details

Product Specific References for Applications and Species

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