Anti-Shank3 Antibody (N367/62)

Name: Anti-Shank3 Antibody
Brand: NeuroMab
SKU: 75-344
Price: 374.0 USD
Availability: InStock

Our Anti-Shank3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N367/62. It detects mouse and rat Shank3, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Adult rat hippocampus immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Shank1, Shank2, Shank3 or untagged Kv2.1 plasmid and probed with N367/62 (left) or N23B/49 (right) TC supe.

Immunoblot against adult rat brain membranes (RBM) and membranes from Shank3 wild-type (WT) and knockout (KO) mice probed with N367/62 (left) or K58/35 (right) TC supe. Mouse brains courtesy of Yuan Mei, Holly Robertson and Guoping Feng (MIT).

Adult rat hippocampus immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-344

Volume: 100 µL

Variant

Price:

$374.00

Quantity:

Product Details

Target

Shank3

Target Description

SH3 and multiple ankyrin repeat domains protein 3, or shank 3 (also known as proline-rich synapse-associated protein 2 (ProSAP2)) is a member of the shank family. Shank 3 binding domains include a SH3 domain, a proline rich domain, a PDZ domain and ankyrin repeat domains. Shank 3 is found in many tissues including heart and spleen, but is most abundantly expressed in brain at the post synaptic density. Shank 3 acts as a scaffold protein anchoring and connecting membrane bound receptors, ion channels and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 3 may play a role in synapse formation and dendritic spine maturation. Mutations in the Shank 3 gene have been associated with neuropsychiatric diseases, including autism spectrm disorder.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N367/62

Isotype

IgG2a

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Shank3 Prosap2

Molecular Weight

190 kDa

Antigen

Fusion protein amino acids 538-626 (SH3 domain) of rat Shank3 (accession number Q9JLU4) produced recombinantly in E. Coli

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2315921

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Does not cross-react with Shank1 or Shank2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

SH3 and multiple ankyrin repeat domains protein 3 (Shank3) (Proline-rich synapse-associated protein 2) (ProSAP2) (SPANK-2)

UniProt Details

UniProt (Human): Q9BYB0
UniProt (Immunogen Species): Q9JLU4

Product Specific References for Applications and Species

Electron Microscopy: Rat
Immunocytochemistry: Rat
Immunohistochemistry: Non-Human Primate
Immunoprecipitation: Mouse
Western Blot: Mouse | Rat

Electron Microscopy: Rat
PMID	Dilution	Publication
27144302	1:250	Tao-Cheng, J.H., et al. 2016. Zinc stabilizes Shank3 at the postsynaptic density of hippocampal synapses. PLoS One, e0153979.

Immunocytochemistry: Rat
PMID	Dilution	Publication
30524232	1:100	Ha, H.T., et al. 2018. Shank and zinc mediate an AMPA receptor subunit switch in developing neurons. Frontiers in Molecular Neuroscience, 405.
26547831	1:400	MacGillavry, H.D., et al. 2016. Shank-cortactin interactions control actin dynamics to maintain flexibility of neuronal spines and synapses.. European Journal of Neuroscience, 179-193.

Immunohistochemistry: Non-Human Primate
PMID	Dilution	Publication
31189958	1:100	Zhou, Y., et al. 2019. Atypical behaviour and connectivity in SHANK3-mutant macaques. Nature , 326-331.

Immunoprecipitation: Mouse
PMID	Dilution	Publication
34852231	not listed	Lautz, J.D., et al. 2021. Synaptic protein interaction networks encode experience by assuming stimulus-specific and brain-region-specific states. Cell Reports, 110076.

Western Blot: Mouse
PMID	Dilution	Publication
38297084	1:1000	Stamenkovic, V, et al. 2024. SRC family kinase inhibition rescues molecular and behavioral phenotypes, but not protein interaction network dynamics, in a mouse model of Fragile X syndrome. Molecular Psychiatry, 1392-1405.
37925173	1:500	Cao, Q, et al. 2023. Longitudinal characterization of behavioral, morphological and transcriptomic changes in a tauopathy mouse model. Aging, 11697-11719.
36587867	not listed	Liu, P., et al. 2022. Degradation of Perineuronal Nets in the Cerebellar Interpositus Nucleus Ameliorated Social Deficits in Shank3-deficient Mice. Neuroscience, 29-38.
35022531	1:1000	Tzanoulinou, S., et al. 2022. Inhibition of Trpv4 rescues circuit and social deficits unmasked by acute inflammatory response in a Shank3 mouse model of Autism. . Molecular Psychiatry, 2080-2094.
33229412	not listed	Pandian, S., et al. 2020. Myosin va brain-specific mutation alters mouse behavior and disrupts hippocampal synapses. eNeuro, .
32019829	1:2000	Perfitt, T.L., et al. 2020. Neuronal L-type calcium channel signaling to the nucleus requires a novel CaMKIIα-Shank3 interaction. Journal of Neuroscience, 2000-2014.
31602784	1:500	Rein, B., et al. 2020. Diminished social interaction incentive contributes to social deficits in mouse models of autism spectrum disorder. Genes, Brain, and Behavior, e12610.
31332372	not listed	Guo, B., et al. 2019. Anterior cingulate cortex dysfunction underlies social deficits in Shank3 mutant mice. Nature Neuroscience, 1223-1234.
30302388	not listed	Drapeau, E., et al. 2018. Behavioral phenotyping of an improved mouse model of Phelan–McDermid syndrome with a complete deletion of the Shank3 gene. eNeuro, .
29531362	1:500	Qin, L., et al. 2018. Social deficits in Shank3-deficient mouse models of autism are rescued by histone deacetylase (HDAC) inhibition. Nature Neuroscience, 564-574.
26687841	not listed	Zhou, Y., et al. 2016. Mice with Shank3 Mutations Associated with ASD and Schizophrenia Display Both Shared and Distinct Defects.. Neuron, 147-162.
26627310	1:500	Berg, J.M., et al. 2015. JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse.. Neuron, 1173-1191.

Western Blot: Rat
PMID	Dilution	Publication
30524232	1:100	Ha, H.T., et al. 2018. Shank and zinc mediate an AMPA receptor subunit switch in developing neurons. Frontiers in Molecular Neuroscience, 405.
28139198	1:500	Harony-Nicolas, H., et al. 2017. Oxytocin improves behavioral and electrophysiological deficits in a novel Shank3-deficient rat.. Elife, e18904.
24089484	1:500	Duffney, L.J., et al. 2013. Shank3 deficiency induces NMDA receptor hypofunction via an actin-dependent mechanism.. Journal of Neuroscience, 15767-15778.

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Anti-Shank3 Antibody (N367/62)

SKU: 75-344

Product Details

Product Specific References for Applications and Species

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