Anti-SynCAM4 Antibody (N244/5)
Our Anti-SynCAM4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N244/5. It is KO validated, detects human, mouse, and rat SynCAM4, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Adult rat sciatic nerve immunofluorescence staining: paranodes labeled with N244/5 (red) surround nodes of Ranvier labeled with Beta-IV spectrin (green) (image on right is cross-section of a similar image of left). Images courtesy of Matt Rasband (Baylor College of Medicine).
Product Specific References for Applications and Species
- Immunocytochemistry: Human | Mouse | Rat
- Immunohistochemistry: Mouse
- Light Microscopy: Mouse
- Western Blot: Human | Mouse | Rat
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 25893857 | 1:100 | Yamana, S., et al. 2015. The Cell Adhesion Molecule Necl-4/CADM4 Serves as a Novel Regulator for Contact Inhibition of Cell Movement and Proliferation.. PLoS One, e0124259. |
| 23611113 | not listed | Sugiyama, H., et al. 2013. Interaction of Necl-4/CADM4 with ErbB3 and integrin α6 β4 and inhibition of ErbB2/ErbB3 signaling and hemidesmosome disassembly.. Genes Cells, 519-528. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 36458527 | 1:300 | Nozawa, O., et al. 2023. Necl2/3-mediated mechanism for tripartite synapse formation. Development, dev200931. |
| 36458527 | 1:300 | Nozawa, O., et al. 2022. A Novel Necl-2/3-mediated Mechanism for Tripartite Synapse Formation. Development, . |
| 31451613 | not listed | Elazar, N., et al. 2019. Coordinated internodal and paranodal adhesion controls accurate myelination by oligodendrocytes. Journal of Cell Biology, 2887-2895. |
| 30389906 | 1:500 | Shacham-Silverberg, V., et al. 2018. Phosphatidylserine is a marker for axonal debris engulfment but its exposure can be decoupled from degeneration. Cell Death and Disease, 1116. |
| 30389906 | 1:500 | Shacham-Silverberg, V., et al. 2018. Phosphatidylserine is a marker for axonal debris engulfment but its exposure can be decoupled from degeneration. Cell Death and Disease, 1116. |
| 25362471 | not listed | Chang, K.J., et al. 2014. Glial ankyrins facilitate paranodal axoglial junction assembly.. Nature Neuroscience, 1673-81. |
| 24127433 | 1:400 | Minis, A., et al. 2014. Subcellular transcriptomics-dissection of the mRNA composition in the axonal compartment of sensory neurons.. Developmental Neurobiology, 365-381. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 26644354 | not listed | Gong, B., et al. 2016. Developing high-quality mouse monoclonal antibodies for neuroscience research–approaches, perspectives and opportunities. New Biotechnology, 551-564. |
| 21518878 | not listed | Susuki, K., et al. 2011. Schwann cell spectrins modulate peripheral nerve myelination.. PNAS: USA, 8009-8014. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 35445918 | 1:100 | Jordans, S., et al. 2022. Age-Dependent Increase in Schmidt-Lanterman Incisures and a Cadm4-Associated Membrane Skeletal Complex in Fatty Acid 2-hydroxylase Deficient Mice: a Mouse Model of Spastic Paraplegia SPG35. Molecular Neurobiology, 3969-3979. |
| 30551998 | not listed | Elazar, N., et al. 2019. Axoglial Adhesion by Cadm4 Regulates CNS Myelination. Neuron, 224-231. |
| 29282769 | not listed | Bang., M.L, et al. 2018. Glial M6B stabilizes the axonal membrane at peripheral nodes of Ranvier. Glia, 801-812. |
| 23825401 | not listed | Golan, N., et al. 2013. Genetic deletion of Cadm4 results in myelin abnormalities resembling Charcot-Marie-Tooth neuropathy.. Journal of Neuroscience, 10950-10961. |
| Light Microscopy: Mouse | ||
| PMID | Dilution | Publication |
| 32452538 | 1:300 | Shiotani, H., et al. 2021. Nectin-2α is localized at cholinergic neuron dendrites and regulates synapse formation in the medial habenula. Journal of Comparative Neurology, 450-477. |
| 29524214 | 1:300 | Shiotani, H., et al. 2018. Localization of nectin-2α at the boundary between the adjacent somata of the clustered cholinergic neurons and its regulatory role in the subcellular localization of the voltage-gated A-type K+ channel Kv4.2 in the medial habenula. Journal of Comparative Neurology, 1527-1549. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 28601637 | not listed | Yamana, S., et al. 2017. Necl-4 enhances the PLCγ-c-Raf-MEK-ERK pathway without affecting internalization of VEGFR2. Biochemical and Biophysical Research Communication, 169-175. |
| 25893857 | 1:1000 | Yamana, S., et al. 2015. The Cell Adhesion Molecule Necl-4/CADM4 Serves as a Novel Regulator for Contact Inhibition of Cell Movement and Proliferation.. PLoS One, e0124259. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 36458527 | 1:1000 | Nozawa, O., et al. 2023. Necl2/3-mediated mechanism for tripartite synapse formation. Development, dev200931. |
| 36458527 | 1:1000 | Nozawa, O., et al. 2022. A Novel Necl-2/3-mediated Mechanism for Tripartite Synapse Formation. Development, . |
| 35445918 | 1:250 | Jordans, S., et al. 2022. Age-Dependent Increase in Schmidt-Lanterman Incisures and a Cadm4-Associated Membrane Skeletal Complex in Fatty Acid 2-hydroxylase Deficient Mice: a Mouse Model of Spastic Paraplegia SPG35. Molecular Neurobiology, 3969-3979. |
| 33215680 | 1:250 | Hardt, R., et al. 2021. Decreased turnover of the CNS myelin protein Opalin in a mouse model of hereditary spastic paraplegia 35. Human Molecular Genetics, 3616-3630. |
| 31262725 | 1:500 | Trotter, J.H., et al. 2019. Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters. Journal of Cell Biology, 2677-2698. |
| 31262725 | 1:500 | Trotter, J.H., et al. 2019. Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters. Journal of Cell Biology, 2677-2698. |
| 30551998 | not listed | Elazar, N., et al. 2019. Axoglial Adhesion by Cadm4 Regulates CNS Myelination. Neuron, 224-231. |
| 30357511 | 1:1000 | Saitoh, Y., et al. 2019. The membrane palmitoylated protein, MPP6, is involved in myelin formation in the mouse peripheral nervous system. Histochemistry and Cell Biology, 385-394. |
| 23825401 | not listed | Golan, N., et al. 2013. Genetic deletion of Cadm4 results in myelin abnormalities resembling Charcot-Marie-Tooth neuropathy.. Journal of Neuroscience, 10950-10961. |
| 23700466 | not listed | Zhu,Y., et al. 2013. Necl-4/SynCAM-4 is expressed in myelinating oligodendrocytes but not required for axonal myelination.. PLoS One, e64264. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 27658374 | 1:1000 | Chen, M.S., et al. 2016. Cadm3 (Necl-1) interferes with the activation of the PI3 kinase/Akt signaling cascade and inhibits Schwann cell myelination in vitro. Glia, 2247-2262. |