Anti-PICK1 Antibody (L20/8)
Our Anti-PICK1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L20/8. It is KO validated, detects human, mouse, and rat PICK1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
![Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with L20/8 (left) or N52A/42 (right) TC supe.](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-040-l20-8-wb-1_{width}x.jpg?v=1626903249)
Adult rat hippocampus immunohistochemistry.
![Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with L20/8 (left) or N52A/42 (right) TC supe.](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-040-l20-8-wb-1_1024x.jpg?v=1626903249)
Product Specific References for Applications and Species
- Immunocytochemistry: Mouse | Rat
- Immunohistochemistry: Human | Mouse
- Immunoprecipitation: Mouse | Rat
- Western Blot: Feline | Human | Mouse | Rat
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 34500285 | 1:100 | Wang, L., et al. 2021. The PICK1/TLR4 complex on microglia is involved in the regulation of LPS-induced sepsis-associated encephalopathy. International immunopharmacology, 108116. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 23918399 | not listed | Anggono, V., et al. 2013. PICK1 interacts with PACSIN to regulate AMPA receptor internalization and cerebellar long-term depression.. PNAS: USA, 13976-13981. |
| 22915106 | not listed | Jaafari, N., et al. 2012. PICK1 mediates transient synaptic expression of GluA2-lacking AMPA receptors during glycine-induced AMPA receptor trafficking.. Journal of Neuroscience, 11618-11630. |
| 21252856 | not listed | Nakamura, Y., et al. 2011. PICK1 inhibition of the Arp2/3 complex controls dendritic spine size and synaptic plasticity.. The EMBO Journal, 719-730. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 33660148 | 1:100 | Zhou, Y., et al. 2021. Protein interacting with C-kinase 1 is involved in epithelial-mesenchymal transformation and suppresses progress of gastric cancer. Medical Oncology, 34. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 34673136 | 1:100 | Mo, Y., et al. 2021. Electroacupuncture prevents LPS- induced neuroinflammation via upregulation of PICK-TLR4 complexes in the microglia of hippocampus. Brain research bulletin, 295-304. |
| 34500285 | 1:100 | Wang, L., et al. 2021. The PICK1/TLR4 complex on microglia is involved in the regulation of LPS-induced sepsis-associated encephalopathy. International immunopharmacology, 108116. |
| Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 33488694 | 2ug | Gu, Y., et al. 2020. Electroacupuncture Attenuates CFA-Induced Inflammatory Pain by Regulating CaMKII. Neural Plasticity, 8861994. |
| 26566795 | 2-4ug | Zhu, J., et al. 2016. Protein Interacting C-Kinase 1 Modulates Surface Expression of P2Y6 Purinoreceptor, Actin Polymerization and Phagocytosis in Microglia.. Neurochemical Research, 795-803. |
| 25904794 | 2-4ug/ml | Wang, Y.N., et al. 2015. Protein Interacting with C-Kinase 1 Deficiency Impairs Glutathione Synthesis and Increases Oxidative Stress via Reduction of Surface Excitatory Amino Acid Carrier 1.. Journal of Neuroscience, 6429-6443. |
| 21496646 | not listed | Zhang, J., et al. 2011. The AAA+ ATPase Thorase regulates AMPA receptor-dependent synaptic plasticity and behavior.. Cell, 284-299. |
| Immunoprecipitation: Rat | ||
| PMID | Dilution | Publication |
| 28951554 | 5ug | Gol, S., et al. 2017. Endocytosis and lysosomal degradation of GluA2/3 AMPARs in response to oxygen/glucose deprivation in hippocampal but not cortical neurons. Scientific Reports, 12318. |
| 28855251 | not listed | Fiuza, M., et al. 2017. PICK1 regulates AMPA receptor endocytosis via direct interactions with AP2 α-appendage and dynamin. . Journal of Cell Biology, 3323-3338. |
| 28404816 | not listed | Rajgor, D., et al. 2017. The PICK1 Ca2+ sensor modulates N-methyl-d-aspartate (NMDA) receptor-dependent microRNA-mediated translational repression in neurons.. Journal of Biological Chemistry, 9774-9786. |
| 25475687 | 2ug | Rocca, D.L., et al. 2015. PICK1 links AMPA receptor stimulation to Cdc42.. Neuroscience Letters, 155-159. |
| 18297063 | 3ug | Rocca, D.L., et al. 2008. Inhibition of Arp2/3-mediated actin polymerization by PICK1 regulates neuronal morphology and AMPA receptor endocytosis.. Nature Cell Biology, 259-271. |
| Western Blot: Feline | ||
| PMID | Dilution | Publication |
| 25668772 | 1:200 | Williams, K., et al. 2015. Experience-dependent central vision deficits: Neurobiology and visual acuity.. Vision Research, 68-78. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 32531799 | not listed | Chen, L., et al. 2020. Islet-cell autoantigen 69 accelerates liver regeneration by downregulating Tgfbr1 and attenuating Tgfβ signaling in mice. FEBS Letters, 2881-2893. |
| 26466675 | not listed | Cockbill, L.M., et al. 2015. Protein interacting with C kinase 1 suppresses invasion and anchorage-independent growth of astrocytic tumor cells.. Molecular Biology of the Cell, 4552-4561. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 34673136 | 1:5000 | Mo, Y., et al. 2021. Electroacupuncture prevents LPS- induced neuroinflammation via upregulation of PICK-TLR4 complexes in the microglia of hippocampus. Brain research bulletin, 295-304. |
| 34500285 | 1:5000 | Wang, L., et al. 2021. The PICK1/TLR4 complex on microglia is involved in the regulation of LPS-induced sepsis-associated encephalopathy. International immunopharmacology, 108116. |
| 34455539 | 1:1000 | Longatti, A., et al. 2021. Arhgap22 Disruption Leads to RAC1 Hyperactivity Affecting Hippocampal Glutamatergic Synapses and Cognition in Mice. Molecular Neurobiology, 6092-6110. |
| 34110073 | 1:2000 | Wickens, M.M., et al. 2021. Sex-specific role for prefrontal cortical protein interacting with C kinase 1 in cue-induced cocaine seeking. Addiction Biology, e13051. |
| 32147734 | 1:1000 | Koch, N., et al. 2020. Syndapin I Loss-of-Function in Mice Leads to Schizophrenia-Like Symptoms. Cerebral Cortex, 4306-4324. |
| 31503067 | 1:2000 | Faccidomo, S., et al. 2020. Pharmacological inhibition of glycogen synthase kinase 3 increases operant alcohol self-administration in a manner associated with altered pGSK-3β, protein interacting with C kinase and GluA2 protein expression in the reward pathway of male C57BL/6J mice. Behavioral Pharmacology, 15-26. |
| 30699097 | not listed | Han, K., et al. 2019. Islet-cell autoantigen 69 mediates the antihyperalgesic effects of electroacupuncture on inflammatory pain by regulating spinal glutamate receptor subunit 2 phosphorylation through protein interacting with C-kinase 1 in mice. Pain, 712-723. |
| 30402043 | not listed | Mo, Y., et al. 2018. PICK1 Deficiency Induces Autophagy Dysfunction via Lysosomal Impairment and Amplifies Sepsis-Induced Acute Lung Injury. Mediators of Inflammation, 6757368. |
| 29471107 | 1:10,000 | Qian, M., et al. 2018. PICK1 deficiency exacerbates sepsis-associated acute lung injury and impairs glutathione synthesis via reduction of xCT. . Free Radical Biology and Medicine, 23-34. |
| 27800545 | not listed | Chenaux, G., et al. 2016. Loss of SynDIG1 Reduces Excitatory Synapse Maturation But Not Formation In Vivo. eNeuro, . |
| 26834556 | 1:1000 | Folci, A., et al. 2016. Myosin IXa Binds AMPAR and Regulates Synaptic Structure, LTP, and Cognitive Function. Frontiers in Molecular Neuroscience, 1. |
| 26566795 | 1:2000 | Zhu, J., et al. 2016. Protein Interacting C-Kinase 1 Modulates Surface Expression of P2Y6 Purinoreceptor, Actin Polymerization and Phagocytosis in Microglia.. Neurochemical Research, 795-803. |
| 26306493 | not listed | He, J., et al. 2015. ICA1L forms BAR-domain complexes with PICK1 and is crucial for acrosome formation in spermiogenesis.. Journal of Cell Science, 3822-3836. |
| 26005851 | 1:1000 | Shin, S., et al. 2015. mGluR5 in the nucleus accumbens is critical for promoting resilience to chronic stress. Nature Neuroscience, 1017-1024. |
| 25559403 | 1:2500 | Pandey, S.P., et al. 2015. Development- and age-related alterations in the expression of AMPA receptor subunit GluR2 and its trafficking proteins in the hippocampus of male mouse brain.. Biogerontology, 317-328. |
| 25547463 | 1:1000 | Li, Q.J., et al. 2015. Loss of ICA69 potentiates long-lasting hyperalgesia after subcutaneous formalin injection into the mouse hindpaw.. Neurochemical Research, 579-590. |
| 24069373 | not listed | Breiderhoff, T., et al. 2013. Sortilin-related receptor SORCS3 is a postsynaptic modulator of synaptic depression and fear extinction. PLoS One, e75006. |
| 23719805 | 1 : 1000 | Hoy, J.L., et al. 2013. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions.. Journal of Neuroscience, 9364-9384. |
| 23630453 | not listed | Cao, M., et al. 2013. PICK1 and ICA69 control insulin granule trafficking and their deficiencies lead to impaired glucose tolerance. PLoS Biology, e1001541. |
| 22369234 | 1:500 | Wilke, S.A., et al. 2012. NeuroD2 regulates the development of hippocampal mossy fiber synapses.. Neural Development, 9. |
| 19483194 | 1:1000 | Labrie, V., et al. 2009. Serine racemase is associated with schizophrenia susceptibility in humans and in a mouse model.. Human Molecular Genetics, 3227-3243. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 35721319 | not listed | Stan, G.F., et al. 2022. Ca 2+ Regulates Dimerization of the BAR Domain Protein PICK1 and Consequent Membrane Curvature. Frontiers in Molecular Neuroscience, 893739. |
| 28855251 | not listed | Fiuza, M., et al. 2017. PICK1 regulates AMPA receptor endocytosis via direct interactions with AP2 α-appendage and dynamin. . Journal of Cell Biology, 3323-3338. |
| 28404816 | 1:500 | Rajgor, D., et al. 2017. The PICK1 Ca2+ sensor modulates N-methyl-d-aspartate (NMDA) receptor-dependent microRNA-mediated translational repression in neurons.. Journal of Biological Chemistry, 9774-9786. |
| 27793771 | 1:1000 | Farooq, M., et al. 2017. Lithium increases synaptic GluA2 in hippocampal neurons by elevating the δ-catenin protein. Neuropharmacology, 426-433. |
| 24358315 | 1:2000 | Wang, Z., et al. 2013. C-terminal domain of ICA69 interacts with PICK1 and acts on trafficking of PICK1-PKCα complex and cerebellar plasticity.. PLoS One, e83862. |
| 23843614 | 1:1000 | Murk, K., et al. 2013. The antagonistic modulation of Arp2/3 activity by N-WASP, WAVE2 and PICK1 defines dynamic changes in astrocyte morphology. Journal of Cell Science, 3873-3883. |
| 22915106 | not listed | Jaafari, N., et al. 2012. PICK1 mediates transient synaptic expression of GluA2-lacking AMPA receptors during glycine-induced AMPA receptor trafficking.. Journal of Neuroscience, 11618-11630. |
| 22623912 | 1:200 | Murphy, K.M., et al. 2012. Cortical development of AMPA receptor trafficking proteins.. Frontiers in Molecular Neuroscience, 65. |
| 21855055 | 1:3000 | Ghasemzadeh, M.B., et al. 2011. Glutamatergic plasticity in medial prefrontal cortex and ventral tegmental area following extended-access cocaine self-administration.. Brain Research, 60-71. |
| 21291912 | not listed | Hadlock, G.C., et al. 2011. Ex vivo identification of protein-protein interactions involving the dopamine transporter.. Journal of Neuroscience Methods, 303-307. |
| 21252856 | not listed | Nakamura, Y., et al. 2011. PICK1 inhibition of the Arp2/3 complex controls dendritic spine size and synaptic plasticity.. The EMBO Journal, 719-730. |
| 20802490 | not listed | Evers, D.M., et al. 2010. Plk2 attachment to NSF induces homeostatic removal of GluA2 during chronic overexcitation.. Nature Neuroscience, 1199-1207. |
| 20403402 | not listed | Shao, X., et al. 2010. Threonine 82 at the PDZ domain of PICK1 is critical for AMPA receptor interaction and localization.. Neurochemistry International, 962-970. |
| 19368820 | 1:3000 | Ghasemzadeh, M.B., et al. 2009. Region-specific alterations in glutamate receptor expression and subcellular distribution following extinction of cocaine self-administration.. Brain Research, 89-102. |
| 19135470 | 1:3000 | Ghasemzadeh, M.B., et al. 2009. Locomotor sensitization to cocaine is associated with distinct pattern of glutamate receptor trafficking to the postsynaptic density in prefrontal cortex: early versus late withdrawal effects.. Pharmacology Biochemistry Behavior, 383-392. |
| 19105975 | 1:300 | Ghasemzadeh, M.B., et al. 2009. Behavioral sensitization to cocaine is associated with increased glutamate receptor trafficking to the postsynaptic density after extended withdrawal period.. Neuroscience, 414-426. |