Our Anti-GOLGA2 (GM130) chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human GOLGA2 (GM130), and is affinity-purified IgY. It is great for use in IHC, ICC, WB.
Immunofluorescent staining of HeLa cells using 1 µg/mL Aves Labs chicken anti-GM130 (GOLGA2) (Cat No. GOLGA2-0100) antibody (green). Actin filaments were stained using Phalloidin (red). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. No. AR-6501). Anti-GM130 specifically stains the Golgi apparatus in HeLa cells.
GOLGA2, also known as GM130, is a Golgi matrix protein critical for the proper functioning and organization of the Golgi apparatus. Discovered in the mid-1990s, GOLGA2 is involved in the maintenance of Golgi structure and the facilitation of vesicle trafficking within the cell. This protein is pivotal in ensuring that proteins and lipids are correctly modified, sorted, and sent to their destined locations, which is essential for various cellular processes including secretion, plasma membrane repair, and lysosome function. GOLGA2's role in cellular homeostasis and its effect on the Golgi apparatus associates it with several neurodegenerative and congenital disorders. Mutations in the gene and dysfunction of the protein can lead to brain development delays, and diseases such as Parkinson’s and ALS (Huang et al. 2021). Also, changes in GOLGA2 levels can affect Golgi structure and vesicle trafficking leading to aberrant cell proliferation, tumorigenesis and metastasis, making GOLGA2 a target of anti-cancer gene therapy (Chang et al. 2012).
Affinity-Purified IgY
200 µg/mL
Polyclonal
IgY
ICC, IHC, WB
Chicken
GOLGA2
130 kDa
Synthetic peptide
Human
AB_3105809
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe HeLa cell lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
