ImmunoHistoMount™
Immunohistomounting medium (IHM) is a permanent aqueous mounting medium designed for permanent mounting of tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that can not be dehydrated with organic solvents.
Immunohistomounting medium (IHM) is a permanent aqueous mounting medium designed for permanent mounting of tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that can not be dehydrated with organic solvents. This mounting medium preserves Fast red, Aminoethylycarbazole (AEC), BCIP/NBT, BCIP/INT chromogens and is also compatible with counterstain like Hematoxylin and Nuclear fast red (NFR). It is also suitable for chromogens like DAB and DAB with nickel and cobalt. It is not compatible with H & E combine staining.
Ready to use mounting medium
Mounting of immunohisto slides that cannot be dehydrated with organic solvents
Room temp; Long term storage 2-8 °C recommended; Protect from light; DO NOT FREEZE
Ships overnight (domestic), International Priority Shipping
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States
- Bring the vial to room temperature. DO NOT USE NON-POLAR SOLVENT BEFORE APPLYING THIS MOUNTING MEDIUM
- Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface.
- Turn the vial upside down and open the dropper to remove any air bubbles.
- Apply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting the slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide against a paper towel.
- Let stand at room temperature for about 1 to 2 hours. Or the slides can be heated at 50oC for 40-60 minutes. The slides are ready for visualization under a microscope.
- To visualize slide under microscope with 4X, 10X, 20X or 40X lens, coverslip is NOT required At this step for any reason if mounting medium has to be removed, follow these instructions: Soak the slide in warm (37oC) distilled or deionized water for 5-10 minutes until the mounting medium is dissolved. Rinse slide with warm water several times to remove all mounting medium; the slide can be remounted again if required.
Product Specific References
| PMID | Publication |
| 33828989 | Scopim-Ribeiro, R., et al. 2021. NSG Mice Facilitate ex vivo Characterization of Ewing Sarcoma Lung Metastasis Using the PuMA Model. Frontiers in Oncology, 645757. |
| 23965613 | Li, L, et al. 2013. Divergent astrovirus associated with neurologic disease in cattle. Emerging Infectious Diseases, 1385-1392. |
| 23628223 | Li, L, et al. 2013. Circovirus in tissues of dogs with vasculitis and hemorrhage. Emerging Infectious Diseases, 534-541. |
| 21051708 | Sanders, ME, et al. 2011. The Streptococcus pneumoniae capsule is required for full virulence in pneumococcal endophthalmitis. Investigative ophthalmology & visual science, 865-872. |
| 20795860 | Norcross, EW, et al. 2010. Assessment of Streptococcus pneumoniae capsule in conjunctivitis and keratitis in vivo neuraminidase activity increases in nonencapsulated pneumococci following conjunctival infection. Current eye research, 787-798. |