ImmunoChemistry Technologies

302 products

ImmunoChemistry Technologies is your partner in cell viability assays, ELISA reagents, IHC reagents, and immunoassay services. With decades of protein chemistry expertise, ICT offers a wide range of products for immunoassay, cellular, and molecular diagnostics development. Our kits include fluorescent whole cell assays for intracellular apoptosis detection, autophagy, oxidative stress, caspase activity, and more. Optimize your technique with our coating and wash buffers, blockers, sample and assay diluents, stabilizers, and stop solutions. From ELISA development and plate coating, to IHC counterstains and mounting media, to antibody conjugation and purification, the team at our ISO-13485-certified facility in Davis, California is dedicated to helping you build a better assay.

Showing 217 - 240 of 302 products

ImmunoChemistry Technologies Recombinant Mouse TNF alpha

$200

ImmunoChemistry Technologies Recombinant Mouse IL-2

$200

ImmunoChemistry Technologies Recombinant Mouse VEGF-A

$200

ImmunoChemistry Technologies Recombinant Mouse APRIL

$200

ImmunoChemistry Technologies Recombinant Mouse IL-1 beta

$200

ImmunoChemistry Technologies Recombinant Human IL-8

$200

ImmunoChemistry Technologies Recombinant Human IL-4

$200

ImmunoChemistry Technologies Recombinant Mouse IL-17A

$200

ImmunoChemistry Technologies Recombinant Human APRIL (TNFSF13)

$200

ImmunoChemistry Technologies Recombinant Human IFN gamma

$200

Figure 1. In Antigen-Down-ELISAs, a Target Antigen coated onto the ELISA plate surface is used to quantify specific antibody concentrations present within samples. When the sample is added, the antigen on the plate is bound by antibodies from the sample, which are then retained in the well. A species-specific antibody labeled with HRP is added, followed by substrate, a stop solution is added, and then the absorbance is read. The higher the signal, the more antibodies there are in the sample.

Figure 2. Initial Assay Feasibility Plate Map. Wells are coated with 100 µL per well of either 1 µg/mL (light gray) or 4 µg/mL (dark gray) antigen coating solution, or Antigen Coating Buffer blank well controls (white). A known Positive Control is diluted out via a series of 1:2 dilutions and added to six sections of the plate. After the antibody capture step, plates are incubated with three different dilutions of the HRP-IgG conjugate, washed, and developed with TMB substrate. See protocol.

ImmunoChemistry Technologies Antigen-Down ELISA Development Kit

$590

ImmunoChemistry Technologies Antibody-Sandwich ELISA Development Kit

$669

ImmunoChemistry Technologies General Assay Diluent, 2X

$562

ImmunoChemistry Technologies General Serum Diluent, 2X

$562

ImmunoChemistry Technologies HRP Conjugate Stabilizer, Mammalian, 1X

From $70.30

Figure 1. HRP-Maleimide-conjugated to thiolated antibody. Our Conjugation-Ready HRP-Maleimide reagent (HRP-M) makes it easy for scientists to create their own detection conjugates. The maleimide is linked to the HRP, which facilitates a stable bond with sulfhydryl (SH) groups on the target antibody (shown) or other target protein.

Figure 2. Thiolation. Conjugation-Ready HRP Maleimide links to proteins through free SH groups, which often must be added to the protein using Traut’s reagent prior to conjugation.

ImmunoChemistry Technologies Conjugation-Ready HRP Maleimide

$227

ImmunoChemistry Technologies HRP Goat Anti-Rabbit IgG Fc

$150

ImmunoChemistry Technologies HRP Goat Anti-Mouse IgG Fc

$150

ImmunoChemistry Technologies HRP Goat Anti-Human IgG Fc

$150

ImmunoChemistry Technologies Pack of Desiccant Packets

ImmunoChemistry Technologies Stop Solution for AP Substrates (STOPP)

$26

ImmunoChemistry Technologies BCIP/NBT 1-Component AP Membrane Substrate (SUBB)

$66

ImmunoChemistry Technologies TMB 1-Component HRP Membrane Substrate (SUBM)

$66

ImmunoChemistry Technologies pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets (SUBP)

$49

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