ImmunoChemistry Technologies

263 products

ImmunoChemistry Technologies is your partner in cell viability assays, ELISA reagents, IHC reagents, and immunoassay services. With decades of protein chemistry expertise, ICT offers a wide range of products for immunoassay, cellular, and molecular diagnostics development. Our kits include fluorescent whole cell assays for intracellular apoptosis detection, autophagy, oxidative stress, caspase activity, and more. Optimize your technique with our coating and wash buffers, blockers, sample and assay diluents, stabilizers, and stop solutions. From ELISA development and plate coating, to IHC counterstains and mounting media, to antibody conjugation and purification, the team at our ISO-13485-certified facility in Davis, California is dedicated to helping you build a better assay.

Showing 193 - 216 of 263 products

ImmunoChemistry Technologies Plasma Sample Diluent

From $57.25

Figure 1. Dilute samples within the detection limit of the ELISA

ImmunoChemistry Technologies General Serum Diluent

From $57.25

ImmunoChemistry Technologies Antibody Coating Buffer, 5X

From $54.25

Figure 1. Use of General Block ELISA Blocking Buffer in Antigen-Down and Sandwich ELISA formats

ImmunoChemistry Technologies General Block ELISA Blocking Buffer

From $45.60

Figure 1. Equalize the matrix with an assay diluent

ImmunoChemistry Technologies Antigen-Down Assay Diluent

From $57.25

ImmunoChemistry Technologies Neptune Assay Diluent

From $57.25

ImmunoChemistry Technologies IgM-Reducing Assay Diluent

From $57.25

ImmunoChemistry Technologies General Assay Diluent

From $57.50

Figure 1. Use of ELISA Blocking Buffer - Non-Mammalian-based in Antigen-Down and Sandwich ELISA formats

ImmunoChemistry Technologies ELISA Blocking Buffer - Non-Mammalian-based

From $54.25

ImmunoChemistry Technologies Costar® 96-Well Black Polystyrene Plate

$8.05

ImmunoChemistry Technologies Costar® 96-Well EIA/RIA Stripwell Plate

$7.30

ImmunoChemistry Technologies Native Human Cathepsin D

$346

ImmunoChemistry Technologies Cathepsin B

$338

Figure 2. Jurkat cells were exposed to 3% formaldehyde for 30 minutes. Following the formaldhehyde treatment, cells were stained with Green Live/ Dead stain and then imaged with a Nikon E800 microscope (DIC overlay shown). Cells with compromised membranes stained green, while cells with intact membranes excluded the Green Live/Dead stain and remained unstained. Data courtesy of Mrs. Tracy Murphy, ICT.

ImmunoChemistry Technologies Green Live/Dead Stain

$102.50

ImmunoChemistry Technologies DAPI Nuclear Stain

$120.50

Figure 1. Jurkat cells were untreated (live) (A) or treated with 90% ethanol (dead) (B). Cells were pelleted by centrifugation (200 x g for 5 minutes) and resuspended in PBS. Cells were then stained with 7-AAD for 10 minutes on ice, and analyzed using an Accuri C6 flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of the treated cells (B) (ICT 226:30-31).

Figure 2. Jurkat cells were exposed to staurosporine to induce apoptosis, then dually stained with the green fluorescent FAM-FLICA poly caspase probe to detect apoptosis via caspase activity, and the red dye 7-AAD to detect necrosis. Early apoptotic cells fluoresce green with FAM-FLICA. Dually stained green and red fluorescing cells are in mid to late stage apoptosis (active caspases and compromised cell membranes). Necrotic cells fluoresce red. (ICT 196:70).

ImmunoChemistry Technologies 7-AAD Red Fluorescent Live/Dead Stain

$172

Figure 1. Staining of Healthy Jurkat Cells Healthy Jurkat cells were stained with CFSE or left unstained and then analyzed by flow cytometry using an Accuri C6 flow cytometer equipped with a FL1 99% attenuation filter. Unstained Jurkat cells (A), stained Jurkat cells (B), and the corresponding overlay (C) are shown.

Figure 2. Identification of CFSE-stained Cells. K562 target cells were stained green with CFSE and then subjected to effector cells. Green stained target cells are easily identifiable when analyzed by FL1 vs. SSC on a flow cytometer. Target cells stained green with CFSE move to the right along the X-axis (FL1) of the plot (right) compared with unstained effector cells. This plot becomes particularly important when gating on target cells that are the same size as effector cells.

ImmunoChemistry Technologies CFSE Green Fluorescent Stain - for cell labelling and proliferation studies

$120.75

Figure 1. Normal Jurkat cells stained with Acridine Orange (AO) show orange lysosomal staining. Cells were stained with 5 µM AO in PBS for 60 minutes at 37°C. Photomicrographs were taken using a Nikon Eclipse E800 photomicroscope using a 460-500 nm excitation filter and a 505-560 nm emission / barrier filter set at 300X. Photo B shows the corresponding DIC image of the cells in A (AO appears faintly). Data courtesy of Dr. Zbigniew Darzynkiewicz (Brander Cancer Research Institute, New York, NY).

Figure 2. MCF-7 cells were treated with camptothecin to induce apoptosis (cat. 6210), stained with AO in PBS, washed, and then photographed with an epifluorescence microscope at 40X using either a blue light excitation (492 nm) with a 540- 550 nm emission filter (A, lysosomes appear yellowish green), or green light excitation (540 nm) with a long pass >640 nm barrier filter (B, lysosomes appear red). Data courtesy of Dr. Zbigniew Darzynkiewicz (Brander Cancer Research Center Institute, New York, NY).

ImmunoChemistry Technologies Acridine Orange Fluorescent Staining Solution

$22.75

Figure 1. MR-(DEVD)2 (cat. 936) and Hoechst 33342 were used to quantify apoptosis in human PASMCs. Cells were treated with a negative control (A), a drug that inhibits proliferation (B), or H2O2 to induce apoptosis (C). Apoptotic cells stained red with cleaved MR-(DEVD)2 and blue with Hoechst. They have less intense blue nuclei than healthy cells, which have bright blue nuclei. Research of Dr. F. Perros, et al., Universite Paris-Sud 11; Clamart; Hopital Henri-Mondor, Creteil, France.

Figure 2. THP-1 cells were stained with Poly (active) Caspase FAM FLICA® Inhibitor Reagent (green), and Hoechst 33342 (Blue). Cells were incubated with staurosporine to induce apoptosis. Two photos were taken and superimposed. Only one cell of the three cells is apoptotic (middle) – it is stained positive for caspase activity with FAM-VAD-FMK. Data courtesy of Dr. Brian W. Lee.

ImmunoChemistry Technologies Hoechst 33342 Fluorescent Nucleic Acid Stain

$22.75

Figure 1. Propidium iodide excitation and emission spectra.

Figure 2. HL-60 cells were treated with a drug, then stained with FLICA® FAM-VAD-FMK poly-caspase inhibitor reagent (green) (Cat. 92) and propidium iodide (red) (PI). Scanning laser cytometer analysis. Four populations of cells were detected: (A) Unstained live cells, (B) red necrotic cells (PI), (C) green and red late apoptotic cells (FAM-FLICA® plus PI), and (D) green early apoptotic cells (FAM-FLICA®). Data courtesy of Dr. Z. Darzynkiewicz, Brander Cancer Center, NY.

ImmunoChemistry Technologies Propidium Iodide Stain

$22.75

ImmunoChemistry Technologies Fixative

$27.95

Figure 1. A pool of Jurkat cells was spiked with FAM-YVAD-FMK (cat. 9146) and divided into separate treatment groups. Starting with 24 hour samples and working backwards, 10 µM Nigericin was added to cells and the samples were incubated at 37°C throughout the induction process. The cells were washed and analyzed on a flow cytometer. The level of caspase-1 activity directly correlated to the period of exposure to Nigericin Data. courtesy of Mrs. Tracy Murphy, ICT (220:78).

ImmunoChemistry Technologies Nigericin

$45.25

ImmunoChemistry Technologies Tumor Necrosis Factor beta

$360

ImmunoChemistry Technologies Tumor Necrosis Factor alpha

$664

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