Anti-Ankyrin-R/G Antibody (N388A/27)

Name: Anti-Ankyrin-R/G Antibody
Brand: NeuroMab
SKU: 75-415
Price: 319.0 USD
Availability: InStock

Our Anti-Ankyrin-R/G mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N388A/27. It detects human, mouse, and rat Ankyrin-R/G, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.

Immunoblot against extracts of COS cells transiently transfected with GFP-tagged Ankyrin-R, Ankyrin-B, Ankyrin-G or untagged Kv2.1 plasmid probed with N388A/27 (left) or N86/8 (right) TC supe.

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-415

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Ankyrin-R/G

Target Description

Ankyrins are scaffold proteins that function to link proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane cytoskeleton. In this manner, ankyrin family proteins play key roles in various processes including cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N388A/27

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

ANK1 ANK

Molecular Weight

200 kDa

Antigen

Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R (accession number P16157) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2493098

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with Ankyrin-BCross-reacts with Ankyrin-G

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Ankyrin-1 (ANK-1) (Ankyrin-R) (Erythrocyte ankyrin)

UniProt Details

UniProt (Human): P16157
UniProt (Immunogen Species): P16157

Product Specific References for Applications and Species

Immunohistochemistry: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
26644354	not listed	Gong, B., et al. 2016. Developing high-quality mouse monoclonal antibodies for neuroscience research - approaches, perspectives and opportunities. New Biotechnology, 551-564.

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Anti-Ankyrin-R/G Antibody (N388A/27)

SKU: 75-415

Product Details

Product Specific References for Applications and Species

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