Anti-BKbeta3a K+ Channel Antibody (N40B/18)

Name: Anti-BKbeta3a K+ Channel Antibody
Brand: NeuroMab
SKU: 75-098
Price: 319.0 USD
Availability: InStock

3 Citations

Our Anti-BKbeta3a K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N40B/18. It detects human, mouse, and rat BKbeta3a K+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Adult rat hippocampus immunohistochemistry.

Immunoblot versus lysates of COS cells transiently transfected with BKBeta3a or Kv2.1 plasmids, probed with N40B/18 TC supe.

Immunoblot versus COS lysate and mouse brain membranes (MBM), probed with N40B/18 (left panel) and N89/34 (right panel) TC supe.

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-098

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

BKbeta3a K+ channel

Target Description

Calcium-activated potassium channel subunit beta-3, Potassium Calcium-Activated Channel Subfamily M Regulatory Beta Subunit 3 or BKBeta3a is encoded by the gene KCNMB3. BKbeta3a can bind to KCNMA1 to form a channel in the calcium activated large conductance (MaxiK channel) family. BKbeta3a acts to modulate the calcium sensitivity and gating kinetics of KCNMA1. It is widely expressed in tissues, and is present in several isoforms. Diseases associated with this gene include Familial Febrile Seizures.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N40B/18

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Kcnmb3

Molecular Weight

32 kDa

Antigen

Fusion protein amino acids 1-49 (unique N-terminus) of mouse BKBeta3a (accession number Q08460) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10672110

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.25

Conjugate

Unconjugated

Specificity

No cross-reactivity against BKBeta1, BKBeta2, BKBeta3b or BKBeta4

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Potassium large conductance calcium-activated channel, subfamily M, beta member 3

UniProt Details

UniProt (Human): Q9NPA1
UniProt (Immunogen Species): Q08460

Product Specific References for Applications and Species

Western Blot: Human | Rat
Additional Publications: Unspecified

Western Blot: Human
PMID	Dilution	Publication
34072205	not listed	Sek, A., et al. 2021. Identification of the Large-Conductance Ca2+-Regulated Potassium Channel in Mitochondria of Human Bronchial Epithelial Cells. Molecules, 3233.

Western Blot: Rat
PMID	Dilution	Publication
22607137	1:200	Zhang, X.L., et al. 2012. Inflammation-induced changes in BK(Ca) currents in cutaneous dorsal root ganglion neurons from the adult rat.. Molecular Pain, 37.

Additional Publications: Unspecified
PMID	Publication
23740188	Linley, J., et al. 2014. Evidence that two distinct crypt cell types secrete chloride and potassium in human colon.. Gut, 472-479.

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Anti-BKbeta3a K+ Channel Antibody (N40B/18)

SKU: 75-098

Product Details

Product Specific References for Applications and Species

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