Our Anti-CASPR2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K67/25. It is KO validated, detects human, mouse, and rat CASPR2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
Immunoblot on adult rat brain membrane (RBM), and adult mouse brain membranes (MBM) from wild-type (WT) and Caspr2 knockout mice. Caspr KO sample courtesy of Drs. Ido Horresh and Ori Peles, The Weizmann Institute of Science.
Contactin-associated protein-like 2 protein, or Caspr2, is a neuronal transmembrane member of the Neurexin family. Structural features of Caspr2 include epidermal growth factor repeats, laminin G domains, F5/8 type C domain, fibrinogen-like domains, and PDZ binding sites. Caspr2 is a cell adhesion molecule as well as a receptor. It is involved in mediating cell–cell interactions in the nervous system and involved in neural-glia interactions and clustering of potassium channels in myelinated axons. It is expressed at the juxaperanodal region of nodes of Ranvier in the CNS and PNS. Changes in expression have been associated with increased susceptibility to autism and epilepsy.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
K67/25
IgG2a
ICC, IHC, IP, WB
Mouse
CNTNAP2 hCG_15499 tcag7.496
180 kDa
Fusion protein 96-1265 (extracellular domain) of human Caspr2 (accession number A0A090N7T7) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2245198
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.7
WB: 1:500
IHC: 1:100
Unconjugated
No cross-reactivity against Caspr/paranodin
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Varea, O, et al. 2015. Synaptic abnormalities and cytoplasmic glutamate receptor aggregates in contactin associated protein-like 2/Caspr2 knockout neurons. Proceedings of the National Academy of Sciences of the United States of America, 6176-6181.
Miyazaki, Y, et al. 2024. Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity. Cell Reports, 113634.
Lysakowski, A, et al. 2011. Molecular microdomains in a sensory terminal, the vestibular calyx ending. The Journal of neuroscience : the official journal of the Society for Neuroscience, 10101-10114.
Lu, Z, et al. 2016. Molecular Architecture of Contactin-associated Protein-like 2 (CNTNAP2) and Its Interaction with Contactin 2 (CNTN2). The Journal of biological chemistry, 24133-24147.
Martín-de-Saavedra, M, et al. 2021. Shed CNTNAP2 ectodomain is detectable in CSF and regulates Ca2+ homeostasis and network synchrony via PMCA2/ATP2B2. Neuron, 627-643.
Ishii, A, et al. 2009. Human myelin proteome and comparative analysis with mouse myelin. Proceedings of the National Academy of Sciences of the United States of America, 1605-14610.
Lobete, M, et al. 2024. A methodology to globally assess ectodomain shedding using soluble fractions from the mouse brain. Frontiers in psychiatry, 1367526.
Miyazaki, Y, et al. 2024. Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity. Cell Reports, 113634.
Martín-de-Saavedra, M, et al. 2021. Shed CNTNAP2 ectodomain is detectable in CSF and regulates Ca2+ homeostasis and network synchrony via PMCA2/ATP2B2. Neuron, 627-643.
Mabb, A, et al. 2016. Topoisomerase 1 Regulates Gene Expression in Neurons through Cleavage Complex-Dependent and -Independent Mechanisms. PloS one, e0156439.
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![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)], cerebellum [HBM(Cb)], and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/files/73-075-k67-25-wb-2_{width}x.jpg?v=1704390077)
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)], cerebellum [HBM(Cb)], and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/files/73-075-k67-25-wb-2_1024x.jpg?v=1704390077)
