Anti-Cav3.2 Ca2+ Channel Antibody (N55/10)

Name: Anti-Cav3.2 Ca2+ Channel Antibody
Brand: NeuroMab
SKU: 75-095
Price: 319.0 USD
Availability: InStock

12 Citations

Our Anti-Cav3.2 Ca2+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N55/10. It is KO validated, detects human and mouse Cav3.2 Ca2+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Immunohistochemistry of coronal brain sections from WT and Cav3.2 KO mice. Reproduced with permission from Mala Shah (University College London, England, UK) and Nature Neuroscience (2011, Huang et al., PMID 21358644).

Stable cell immunoblot: extracts of HEK cells stably-expressing Flag-tagged Cav3.2, Cav3.1 or untagged Kv2.1 plasmid and probed with N55/10 TC supe (left) or Rabbit anti-Flag (right).

Species Reactivity

Human, Mouse

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-095

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Cav3.2 Ca2+ channel

Target Description

Voltage-dependent T-type calcium channel subunit alpha-1H or Cav3.2 Ca2+ channel is encoded by the gene CACNA1H. Cav3.2 is a pore forming alpha subunit of T voltage-sensitive calcium channels and gives rise to T-type calcium currents. (transient opening calcium channels). These channels facilitate entry of calcium ions into excitable cells are involved in a variety of calcium dependent processes, including neurotransmitter release, muscle contraction, and gene expression. Cav3.2 is expressed in kidney, liver, and heart and brain.as a membrane bound protein. Diseases associated with this gene include Hyperaldosteronism and Epilepsy.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N55/10

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

CACNA1H

Molecular Weight

260 kDa

Antigen

Fusion protein amino acids 1019-1293 (cytoplasmic loop between repeat II and repeat III) of human Cav3.2 (accession number O95180), epitope mapped to amino acids 1179-1192 (AEDGRAAPGPRATP) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse

Antibody Registry ID

AB_2315827

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.22

Conjugate

Unconjugated

Specificity

Does not cross-react with Cav3.1

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Voltage-dependent T-type calcium channel subunit alpha-1H (Low-voltage-activated calcium channel alpha1 3.2 subunit) (Voltage-gated calcium channel subunit alpha Cav3.2)

UniProt Details

UniProt (Human): O95180
UniProt (Immunogen Species): O95180

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Mouse | Rat | Tree Shrew
Western Blot: Human | Mouse | Tree Shrew
Additional Publications: Unspecified

Immunocytochemistry: Mouse
PMID	Dilution	Publication
25825486	not listed	Curran, J., et al. 2015. Eps15 Homology Domain-containing Protein 3 Regulates Cardiac T-type Ca2+ Channel Targeting and Function in the Atria.. The Journal of Biological Chemistry, 12210-12221.

Immunohistochemitry: Mouse
PMID	Dilution	Publication
33863995	1:500	Shen, M., et al. 2021. FXR1 regulation of parvalbumin interneurons in the prefrontal cortex is critical for schizophrenia-like behaviors. Molecular Psychiatry, 6845-6867.
27616982	not listed	Aguado, C., et al. 2016. Ontogenic Changes and Differential Localization of T-type Ca(2+) Channel Subunits Cav3.1 and Cav3.2 in Mouse Hippocampus and Cerebellum. Frontiers in Neuroanatomy, 83.
27353765	1:50	Liu, D.D., et al. 2016. Growth differentiation factor-15 promotes glutamate release in medial prefrontal cortex of mice through upregulation of T-type calcium channels. Scientific Reports, 28653.
25578363	not listed	Martinello, K., et al. 2015. Cholinergic afferent stimulation induces axonal function plasticity in adult hippocampal granule cells.. Neuron, 346-363.
21358644	not listed	Huang, Z., et al. 2011. Presynaptic HCN1 channels regulate Cav3.2 activity and neurotransmission at select cortical synapses. Nature Neuroscience, 478-486.

Immunohistochemitry: Rat
PMID	Dilution	Publication
25578363	not listed	Martinello, K., et al. 2015. Cholinergic afferent stimulation induces axonal function plasticity in adult hippocampal granule cells.. Neuron, 346-363.

Immunohistochemitry: Tree Shrew
PMID	Dilution	Publication
22114295	1:200	Wei, H., et al. 2011. Thalamic burst firing propensity: a comparison of the dorsal lateral geniculate and pulvinar nuclei in the tree shrew.. Journal of Neuroscience, 17287-17299.

Western Blot: Human
PMID	Dilution	Publication
28742985	0.5ug/ml	Cain, S.M., et al. 2017. GABAB receptors suppress burst-firing in reticular thalamic neurons. Channels, 574-586.
24362252	1:500	Dziegielewska, B., et al. 2014. T-type Ca2+ channel inhibition induces p53-dependent cell growth arrest and apoptosis through activation of p38-MAPK in colon cancer cells. Molecular Cancer Research, 348-358.
21362533	not listed	Feiner, E.C., et al. 2010. Left ventricular dysfunction in murine models of heart failure and in failing human heart is associated with a selective decrease in the expression of caveolin-3.. Journal of Cardiac Failure, 253-263.

Western Blot: Mouse
PMID	Dilution	Publication
31910261	not listed	Lee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033.
27353765	1:200	Liu, D.D., et al. 2016. Growth differentiation factor-15 promotes glutamate release in medial prefrontal cortex of mice through upregulation of T-type calcium channels. Scientific Reports, 28653.
25825486	not listed	Curran, J., et al. 2015. Eps15 Homology Domain-containing Protein 3 Regulates Cardiac T-type Ca2+ Channel Targeting and Function in the Atria.. The Journal of Biological Chemistry, 12210-12221.
21358644	1:250	Huang, Z., et al. 2011. Presynaptic HCN1 channels regulate Cav3.2 activity and neurotransmission at select cortical synapses. Nature Neuroscience, 478-486.

Western Blot: Tree Shrew
PMID	Dilution	Publication
22114295	1:1000	Wei, H., et al. 2011. Thalamic burst firing propensity: a comparison of the dorsal lateral geniculate and pulvinar nuclei in the tree shrew.. Journal of Neuroscience, 17287-17299.

Additional Publications: Unspecified
PMID	Publication
23287412	Valerie, N.C., et al. 2013. Inhibition of T-type calcium channels disrupts Akt signaling and promotes apoptosis in glioblastoma cells.. Biochem Pharmacol., 888-897.

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Anti-Cav3.2 Ca2+ Channel Antibody (N55/10)

SKU: 75-095

Product Details

Product Specific References for Applications and Species

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