Anti-Fig4/Sac3 Antibody (N202/7)
Our Anti-Fig4/Sac3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N202/7. It is KO validated, detects human and mouse Fig4/Sac3, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
![Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N202/7 (left) or N52A/42 (right) TC supe.](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-201-n202-7-wb3_8e8da8a0-c1e9-490d-88e5-8c7bfe62cd70_{width}x.jpg?v=1626904076)
Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N202/7 (left) or N52A/42 (right) TC supe.
![Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N202/7 (left) or N52A/42 (right) TC supe.](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-201-n202-7-wb3_8e8da8a0-c1e9-490d-88e5-8c7bfe62cd70_1024x.jpg?v=1626904076)
Product Specific References for Applications and Species
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 29518270 | not listed | Hu, B., et al. 2018. Myelin abnormality in Charcot-Marie-Tooth type 4J recapitulates features of acquired demyelination. Annals of Neurology, 756-770. |
| 26807988 | 1:200 | Jones, J.M., et al. 2016. Single amino acid deletion in transmembrane segment D4S6 of sodium channel Scn8a (Nav1.6) in a mouse mutant with a chronic movement disorder. Neurobiology Disorders, 36-45. |
| 22028665 | not listed | Vaccari, I., et al. 2011. Genetic interaction between MTMR2 and FIG4 phospholipid phosphatases involved in Charcot-Marie-Tooth neuropathies.. PLOS Genetics, e1002319. |
| Immunoprecipitation: Human | ||
| PMID | Dilution | Publication |
| 26519381 | 0.4-10ug/ml | Cottarel, J., et al. 2016. FIG4 is a hepatitis C virus particle-bound protein implicated in virion morphogenesis and infectivity with cholesteryl ester modulation potential. Journal of General Virology, 69-81. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 38725096 | not listed | Zhao, M, et al. 2024. Coxiella burnetii effector CvpE maintains biogenesis of Coxiella-containing vacuoles by suppressing lysosome tubulation through binding PI(3)P and perturbing PIKfyve activity on lysosomes. Virulence, 2350893. |
| 31313076 | not listed | Shisheva, A., et al. 2019. Severe Consequences of SAC3/FIG4 Phosphatase Deficiency to Phosphoinositides in Patients with Charcot-Marie-Tooth Disease Type-4J. Molecular Neurobiology, 8656-8667. |
| 28859335 | not listed | Gentil, B.J., et al. 2017. A New Mutation in FIG4 Causes a Severe Form of CMT4J Involving TRPV4 in the Pathogenic Cascade. Journal of Neuropathology and Experimental Neurology, 789-799. |
| 25239001 | not listed | de Kovel, C.G., et al. 2014. Characterization of a de novo SCN8A mutation in a patient with epileptic encephalopathy.. Epilepsy Research, 1511-1518. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 28859335 | not listed | Gentil, B.J., et al. 2017. A New Mutation in FIG4 Causes a Severe Form of CMT4J Involving TRPV4 in the Pathogenic Cascade. Journal of Neuropathology and Experimental Neurology, 789-799. |
| 28552585 | not listed | Morioka, S., et al. 2017. Phosphoinositide phosphatase Sac3 regulates the cell surface expression of scavenger receptor A and formation of lipid droplets in macrophages. Experimental Cell Research, 252-259. |
| 27008179 | 1:200 | Mironova, Y.A., et al. 2016. PI (3, 5) P2 biosynthesis regulates oligodendrocyte differentiation by intrinsic and extrinsic mechanisms.. Elife, e13023. |
| 25187576 | not listed | Vaccari, I., et al. 2015. Loss of Fig4 in both Schwann cells and motor neurons contributes to CMT4J neuropathy.. Human Molecular Genetics, 383-396. |
| 24598713 | not listed | Baulac, S.,, et al. 2014. Role of the phosphoinositide phosphatase FIG4 gene in familial epilepsy with polymicrogyria. Neurology, 1068-1075. |
| 24004519 | 1:1000 | Reifler, A., et al. 2013. Murine Fig4 is dispensable for muscle development but required for muscle function.. Skelet Muscle., 21. |
| 23047693 | 1:500 | Zolov, S.N., et al. 2012. In vivo, Pikfyve generates PI(3,5)P2, which serves as both a signaling lipid and the major precursor for PI5P.. PNAS USA, 17472-17477. |
| 22998443 | 1:200 | Tahtouh, T., et al. 2012. Selectivity, cocrystal structures, and neuroprotective properties of leucettines, a family of protein kinase inhibitors derived from the marine sponge alkaloid leucettamine B.. Journal of Medicinal Chemistry, 9312-9330. |
| 21872275 | not listed | Yan, Q., et al. 2012. Trauma does not accelerate neuronal degeneration in Fig4 insufficient mice.. Journal of the Neurological Sciences, 102-107. |
| 21655088 | 1:200 | Lenk, G.M., et al. 2011. Pathogenic mechanism of the FIG4 mutation responsible for Charcot-Marie-Tooth disease CMT4J.. PLOS Genetics, e1002104. |