Anti-GABA-A-R-Alpha5 Antibody (N415/24)

12 Citations

Our Anti-GABA-A-R-Alpha5 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N415/24. It is KO validated, detects human and mouse GABA-A-R-Alpha5, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot against crude low-speed pellet fractions from adult rat brain (RBLSP) and wild-type (WT) and GABA-A-R- Alpha5 knockout (KO) mouse brains probed with N415/24 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Uwe Rudolph (Harvard Medical School).
Immunolabelling showing α1-/α5-containing GABAA receptors demonstrated synaptic localizations in a primary hippocampal neuronal culture. Distributions of α1 and α5 (cat. 75-401, 1:1000) subunits (A,B) were co-stained with the inhibitory presynaptic marker protein vGat (C). (D) The α1 subunit was mostly synaptically localized, opposing vGat, whereas α5 was distributed at both the soma and synapse and often showed co-localization with α1-vGat puncta at the synapse (E,F). CC-BY-4.0. PMID:38201218
Immunoblot against crude low-speed pellet fractions from adult rat brain (RBLSP) and wild-type (WT) and GABA-A-R- Alpha5 knockout (KO) mouse brains probed with N415/24 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Uwe Rudolph (Harvard Medical School).
Immunoblot against crude low-speed pellet fractions from adult rat brain (RBLSP) and wild-type (WT) and GABA-A-R- Alpha5 knockout (KO) mouse brains probed with N415/24 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Uwe Rudolph (Harvard Medical School).
Immunolabelling showing α1-/α5-containing GABAA receptors demonstrated synaptic localizations in a primary hippocampal neuronal culture. Distributions of α1 and α5 (cat. 75-401, 1:1000) subunits (A,B) were co-stained with the inhibitory presynaptic marker protein vGat (C). (D) The α1 subunit was mostly synaptically localized, opposing vGat, whereas α5 was distributed at both the soma and synapse and often showed co-localization with α1-vGat puncta at the synapse (E,F). CC-BY-4.0. PMID:38201218

Human, Mouse, Rat
ICC, IHC, WB
Mouse
490
550
594
650

Go to Full Product Details

Data Sheet
KO Validated KO Validated

SKU: 75-401

Volume: 100 µL
1-2 business days
Price:
$319.00

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
382012181:1000Chen, M, et al. 2023. Brain Region Differences in α1- and α5-Subunit-Containing GABAA Receptor Proteomes Revealed with Affinity Purification and Blue Native PAGE Proteomics. Cells, 14.
Western Blot: Human
PMID Dilution Publication
299618701:500Butler, K.M., et al. 2018. De novo variants in GABRA2 and GABRA5 alter receptor function and contribute to early-onset epilepsy. Brain, 2392-2405.
Western Blot: Mouse
PMID Dilution Publication
377595271:500Gruol, DL, et al. 2023. Impact of Elevated Brain IL-6 in Transgenic Mice on the Behavioral and Neurochemical Consequences of Chronic Alcohol Exposure. Cells, 0.
367750971:500Gruol, D.L., et al. 2023. Neuroimmune Interactions with Binge Alcohol Drinking in the Cerebellum of IL-6 Transgenic Mice. Neuropharmacology, 109455.
357322721:1000McCoy, A.M., et al. 2022. Positive Allosteric Modulation of α5-GABAA Receptors Reverses Stress-Induced Alterations in Dopamine System Function and Prepulse Inhibition of Startle. The International Journal of Neuropsychopharmacology, 688-698.
33096025not listedDavenport, C.M., et al. 2021. Relocation of an extrasynaptic GABAA receptor to inhibitory synapses freezes excitatory synaptic strength and preserves memory. Neuron, 123-134.
326345321 : 500Gruol, D.L., et al. 2020. Alcohol and IL-6 Alter Expression of Synaptic Proteins in Cerebellum of Transgenic Mice With Increased Astrocyte Expression of IL-6. Neuroscience, 124-137.
314375341:500Roberts, A.J., et al. 2019. Increased IL-6 expression in astrocytes is associated with emotionality, alterations in central amygdala GABAergic transmission, and excitability during alcohol withdrawal. Brain Behavior and Immunity, 188-202.
297877381:500Gruol, D.L., et al. 2018. Altered brain activity during withdrawal from chronic alcohol is associated with changes in IL-6 signal transduction and GABAergic mechanisms in transgenic mice …. Neuropharmacology, 32-46.
26644354not listedGong, B., et al. 2016. Developing high-quality mouse monoclonal antibodies for neuroscience research–approaches, perspectives and opportunities. Nano Biotechnology, 551-564.
Western Blot: Rat
PMID Dilution Publication
325799171:1000Rajgor, D., et al. 2020. Local miRNA-dependent translational control of GABAAR synthesis during inhibitory long-term potentiation. Cell reports, 31(12), p.107785.. Cell reports, 107785.
26644354not listedGong, B., et al. 2016. Developing high-quality mouse monoclonal antibodies for neuroscience research–approaches, perspectives and opportunities. Nano Biotechnology, 551-564.

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