Anti-GABA-A-R-Alpha5 Antibody (N415/24)
Our Anti-GABA-A-R-Alpha5 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N415/24. It is KO validated, detects human and mouse GABA-A-R-Alpha5, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Immunoblot against crude low-speed pellet fractions from adult rat brain (RBLSP) and wild-type (WT) and GABA-A-R- Alpha5 knockout (KO) mouse brains probed with N415/24 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Uwe Rudolph (Harvard Medical School).
Product Specific References for Applications and Species
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 38201218 | 1:1000 | Chen, M, et al. 2023. Brain Region Differences in α1- and α5-Subunit-Containing GABAA Receptor Proteomes Revealed with Affinity Purification and Blue Native PAGE Proteomics. Cells, 14. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 29961870 | 1:500 | Butler, K.M., et al. 2018. De novo variants in GABRA2 and GABRA5 alter receptor function and contribute to early-onset epilepsy. Brain, 2392-2405. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 37759527 | 1:500 | Gruol, DL, et al. 2023. Impact of Elevated Brain IL-6 in Transgenic Mice on the Behavioral and Neurochemical Consequences of Chronic Alcohol Exposure. Cells, 0. |
| 36775097 | 1:500 | Gruol, D.L., et al. 2023. Neuroimmune Interactions with Binge Alcohol Drinking in the Cerebellum of IL-6 Transgenic Mice. Neuropharmacology, 109455. |
| 35732272 | 1:1000 | McCoy, A.M., et al. 2022. Positive Allosteric Modulation of α5-GABAA Receptors Reverses Stress-Induced Alterations in Dopamine System Function and Prepulse Inhibition of Startle. The International Journal of Neuropsychopharmacology, 688-698. |
| 33096025 | not listed | Davenport, C.M., et al. 2021. Relocation of an extrasynaptic GABAA receptor to inhibitory synapses freezes excitatory synaptic strength and preserves memory. Neuron, 123-134. |
| 32634532 | 1 : 500 | Gruol, D.L., et al. 2020. Alcohol and IL-6 Alter Expression of Synaptic Proteins in Cerebellum of Transgenic Mice With Increased Astrocyte Expression of IL-6. Neuroscience, 124-137. |
| 31437534 | 1:500 | Roberts, A.J., et al. 2019. Increased IL-6 expression in astrocytes is associated with emotionality, alterations in central amygdala GABAergic transmission, and excitability during alcohol withdrawal. Brain Behavior and Immunity, 188-202. |
| 29787738 | 1:500 | Gruol, D.L., et al. 2018. Altered brain activity during withdrawal from chronic alcohol is associated with changes in IL-6 signal transduction and GABAergic mechanisms in transgenic mice …. Neuropharmacology, 32-46. |
| 26644354 | not listed | Gong, B., et al. 2016. Developing high-quality mouse monoclonal antibodies for neuroscience research–approaches, perspectives and opportunities. Nano Biotechnology, 551-564. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 32579917 | 1:1000 | Rajgor, D., et al. 2020. Local miRNA-dependent translational control of GABAAR synthesis during inhibitory long-term potentiation. Cell reports, 31(12), p.107785.. Cell reports, 107785. |
| 26644354 | not listed | Gong, B., et al. 2016. Developing high-quality mouse monoclonal antibodies for neuroscience research–approaches, perspectives and opportunities. Nano Biotechnology, 551-564. |