Anti-Olig1 Antibody (N149/25)

Name: Anti-Olig1 Antibody
Brand: NeuroMab
SKU: 75-180
Price: 319.0 USD
Availability: InStock

Our Anti-Olig1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N149/25. It detects mouse Olig1, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Immunofluorescence of postnatal day 17 mouse corpus callosum (CC) near lateral ventricle (LV). Data courtesy of Hui-Hsin Tsai and David Rowitch, UCSF.

Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Olig1, Olig2 and untagged Kv2.1 plasmids and probed with N149/25 TC supe (left) and mouse anti-Flag (right.

Species Reactivity

Mouse

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-180

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Olig1

Target Description

Oligodendrocyte transcription factor 1, Basic Domain, Helix-Loop-Helix Protein, Class B, 6 or Class E Basic Helix-Loop-Helix Protein 21 is encoded by the gene Olig1. Olig1 is a transcription factor and a member of the basic helix-loop-helix (bHLH) factor family. Olig1 promotes formation and maturation of oligodendrocytes (oligodendrogensis). Olig1 is expressed in spinal cord and brain during development, and by oligodendrocytes after late embryonic development. Olig1 is a useful marker for Oligodendrocyte staining. Diseases associated with this gene include Oligodendroglioma and Grade Iii Astrocytoma.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N149/25

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Olig1

Molecular Weight

30 kDa

Antigen

Fusion protein amino acids 1-67 (N-terminus) of mouse Olig1 (accession number Q9JKN5) produced recombinantly in E. Coli

Species Reactivity

Mouse

Antibody Registry ID

AB_2157532

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.108

Conjugate

Unconjugated

Specificity

No cross-reactivity against Olig2

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Oligodendrocyte transcription factor 1 (Oligo1) (Oligodendrocyte-specific bHLH transcription factor 1)

UniProt Details

UniProt (Human): Q8TAK6
UniProt (Immunogen Species): Q9JKN5

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse
Immunohistochemistry: Mouse

Additional Publications: Unspecified

Immunocytochemistry: Human
PMID	Dilution	Publication
27110916	1:100	Xue, Y., et al. 2016. Sequential regulatory loops as key gatekeepers for neuronal reprogramming in human cells.. Nature Neuroscience, 807-15.
23313552	1:100	Xue, Y., et al. 2013. Direct conversion of fibroblasts to neurons by reprogramming PTB-regulated microRNA circuits.. Cell, 82-96.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
22308465	1:1000	Lujan, E., et al. 2012. Direct conversion of mouse fibroblasts to self-renewing, tripotent neural precursor cells.. PNAS USA, 2527-2532.
20107439	1:100	Vierbuchen, T., et al. 2010. Direct conversion of fibroblasts to functional neurons by defined factors.. Nature, 1035-1041.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
36214451	1:1000	Merour, E., et al. 2022. Transient regulation of focal adhesion via Tensin3 is required for nascent oligodendrocyte differentiation. Elife, e80273.
33046105	1:300	Ma, Q., et al. 2020. Oligodendrocyte-specific Argonaute profiling identifies microRNAs associated with experimental autoimmune encephalomyelitis.. Journal of Neuroinflammation, 45306.
23884950	1:200	Ehmsen, J.T., et al. 2013. D-serine in glia and neurons derives from 3-phosphoglycerate dehydrogenase.. Journal of Neuroscience, 12464-12469..
22674276	1:50 (supe)	Lu, P.P., et al. 2012. A critical cell-intrinsic role for serum response factor in glial specification in the CNS.. Journal of Neuroscience, 8012-8023.

Additional Publications: Unspecified
PMID	Publication
27888476	Ikeda, M., et al. 2018. Histological detection of dynamic glial responses in the dysmyelinating Tabby-jimpy mutant brain. Anatomical Science International, 119-127.

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Anti-Olig1 Antibody (N149/25)

SKU: 75-180

Product Details

Product Specific References for Applications and Species

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