Our Anti-Pan-Neurofascin (extracellular) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone A12/18. It detects human, mouse, non-human primate, and rat Pan-Neurofascin (extracellular), and is purified by Protein A chromatography. It is great for use in IHC, ICC.
Axon initial segment (AIS) label of rat hippocampal neurons using Anti-Pan-Neurofascin (Extracellular) Antibody FL490 Conjugate (cat. 75-172-FL490, 1:250) in live at DIV6. Image kindly provided by Xuesi Zhou, Interdisciplinary Institute for Neuroscience, France.
Neurofascin is encoded by the gene NFASC. Two major isoforms of NeuroFascin are expressed as a result of alternative splicing, NF186 and NF155. These proteins are expressed in the central nervous system. In brain, NF186 is expressed at Nodes of Ranvier andNF155 is expressed in unmyelinated axons. Neuofascin plays a role in cell adhesion and is thought to be involved in processes such as neurite extension, synaptogenesis and myelination. Diseases associated with this gene include Demyelinating Polyneuropathy.
Purified by Protein A chromatography
0.5 mg/mL
Monoclonal
A12/18
IgG2a
ICC, IHC
Mouse
Nfasc
155, 186 kDa
Lipid raft fractions isolated from adult rat brain. Target identified as Neurofascin (accession number P97685) by: -binding to NF-186-tranfected COS cells- recognition of NF155 and NF186 but not other highly related L1 CAMs like NrCAM- immunoprecipitation of NF155 and NF186 and confirmation by mass spectrometry - loss of immunostaining against NF186-shRNA knockdown samples Rat: amino acids 25-1110 (extracellular domain common to NF155 and NF186);
Human, Mouse, Non-Human Primate, Rat
AB_2939632
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
PBS with 0.09% azide
FL490 Ex: 491 nm, Em: 515 nm
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
