Anti-Rhodopsin Antibody (1D4)

Name: Anti-Rhodopsin Antibody
Brand: PhosphoSolutions
SKU: 1840-RHO
Price: 430.0 USD
Availability: InStock

4 Citations

Our Anti-Rhodopsin primary antibody from PhosphoSolutions is mouse monoclonal. It detects amphibians and most mammals Rhodopsin and is Protein G purified. It is great for use in WB, IHC.

Western blot of rat retina showing specific labeling of the ~39 kDa rhodopsin protein.

Immunofluorescence of mouse retinal section showing specific immunolabeling of the rhodopsin protein(cat. 1840-RHO, red, 1:100) in the rod spherules. Photo courtesy Mary Raven, University of California, Santa Barbara, CA.

Species Reactivity

Blind Mole, Bovine, Canine, Feline, Frog, Gerbil, Goat, Guinea Pig, Hamster, Horse, Human, Monkey, Mouse, Non-Human Primate, Pig, Polar Bear, Rabbit, Rat, Sheep, Tree Shrew, Vole, Whale, Xenopus

Applications

IHC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 1840-RHO

Volume: 100 µL

Variant

Price:

$430.00

Quantity:

Product Details

Target

Rhodopsin

Target Description

Rhodopsin is a photoreceptor protein found in retinal rods. It is a complex formed by the binding of retinaldehyde, the oxidized form of retinol, to the protein opsin and undergoes a series of complex reactions in response to visible light resulting in the transmission of nerve impulses to the brain. Mutation of the rhodopsin gene is a major contributor to various retinopathies such as retinitis pigmentosa. The disease-causing protein generally aggregates with ubiquitin in inclusion bodies, disrupts the intermediate filament network and impairs the ability of the cell to degrade non-functioning proteins which leads to photoreceptor apoptosis (Berson et al., 1991). Other mutations on rhodopsin lead to X-linked congenital stationary night blindness, mainly due to constitutive activation, when the mutations occur around the chromophore binding pocket of rhodopsin (Dryja et al.,1993). Several other pathological states relating to rhodopsin have been discovered including poor post-Golgi trafficking, dysregulative activation, rod outer segment instability and arrestin binding.

Format

Protein G Purified

Clonality

Monoclonal

Clone

1D4

Isotype

IgG1

Applications

IHC, WB

Host Species

Mouse

Gene Name

RHO

Molecular Weight

39 kDa

Antigen

Purified native bovine rhodopsin.

Species Reactivity

Blind Mole, Bovine, Canine, Feline, Frog, Gerbil, Goat, Guinea Pig, Hamster, Horse, Human, Monkey, Mouse, Non-Human Primate, Pig, Polar Bear, Rabbit, Rat, Sheep, Tree Shrew, Vole, Whale, Xenopus

Antibody Registry ID

AB_2492232

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:4000
IHC: 1:100-1:200

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~39 kDa rhodopsin protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

CSNBAD1 antibody, MGC138309 antibody, MGC138311 antibody, OPN 2 antibody, OPN2 antibody, opsd antibody, OPSD_HUMAN antibody, opsin 2 antibody, Opsin 2 rod pigment antibody, Opsin-2 antibody, Opsin2 antibody, Retinitis Pigmentosa 4 antibody, Retinitis pigmentosa 4 autosomal dominant antibody, RHO antibody, Rhodopsin antibody, RP 4 antibody, RP4 antibody

UniProt Details

UniProt (Human): P08100
UniProt (Immunogen Species): P02699

NCBI Gene ID

509933

Product Specific References for Applications and Species

Immunohistochemistry: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
31254513	1:300	Argyriou, C., et al. 2019. A longitudinal study of retinopathy in the PEX1-Gly844Asp mouse model for mild Zellweger Spectrum Disorder. Experimental Eye Research, p.107713.
31145883	1:200	Cho, S.H., et al. 2019. Targeted deletion of Crb1/Crb2 in the optic vesicle models key features of leber congenital amaurosis 8. Developmental biology, May 28. pii: S0012-1606(19)30027-2.
27809820	not listed	Cho, S.H., et al. 2016. Neonatal disease environment limits the efficacy of retinal transplantation in the LCA8 mouse model. BMC ophthalmology, 16(1), 193.
27616714	1:200	Kim, J.Y., et al. 2016. Yap is essential for retinal progenitor cell cycle progression and RPE cell fate acquisition in the developing mouse eye. Developmental Biology. Nov 15;419(2):336-347.

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Product Specific Protocols

Western Blot Protocol: Download

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Anti-Rhodopsin Antibody (1D4)

SKU: 1840-RHO

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Rhodopsin Antibody

Product Specific Protocols

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