Monoclonal Antibodies

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Western blotting of rat brain homogenate (10 ug/lane) with anti-GAD65/67 (L127/8)  at 1:100 dilution and detected with anti-mouse HRP.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Immunoblot against extracts of COS cells transiently transfected with HA-tagged TrpM6 or untagged Kv2.1 plasmid probed with N470/22 (left) or rabbit anti-HA (right)..
Adult rat hippocampus (left) and cerebellum (right) immunohistochemistry.
Immunofluorescence of COS cells expressing Myc/Flag-tagged mouse Kv6.1/KCNG1.  Green = N474/27, red = Rabbit anti-Myc and blue = Hoechst nuclear stain..
Adult rat brain membrane immunoblot.
Adult rat brain membrane immunoblot
Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N456/39 (left) or N52A/42 (right) TC supe.
Adult rat brain membrane immunoblot.
Adult mouse brain membrane (MBM) and transfected cell immunoblot: extracts of MBM and COS cells transiently transfected with Neuroligin-1 and Neuroligin-4* plasmids and probed with N98/47. Data courtesy of Marc Bolliger and Tom Sudhof, UTSW (now at Stanford).

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