Monoclonal Antibodies

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Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N309A/21 TC supe (left) and a rabbit polyclonal antibody control (right).
"Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N307/12 TC supe (left) and a rabbit polyclonal control (right). ."
Tissue and transfected cell immunoblot: extracts of rat eye and brain membrane (REM and RBM, respectively) and COS cells transiently transfected with Myc-tagged BBS5 or untagged Kv2.1 plasmid and probed with N304B/115 (left) and N52A/42 (right) TC supe.
Serial dilutions of BSA-conjugated target and non-target peptides dotted onto membrane and probed with N310/21 TC supe (left) and a rabbit polyclonal antibody control (right).
Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N299/39 TC supe (left) and a mouse loading control (right).
Adult rat brain immunohistochemistry.Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with Flag-tagged BBS3/Arl6 or untagged Kv2.1 plasmid and probed with N302/10 (left) and K89/34 (right) TC supe.
Immunofluorescence staining of adult spinal cord sections from Brevican wild-type (WT) and knockout (KO) mice with N294A/10 (red) and Caspr rabbit polyclonal (green). Images courtesy of Kae-Jiun Chang and Matt Rasband (Baylor College of Medicine).
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N291C/22 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Dane Chetkovich (Northwestern University).Transfected cell immunoblot: extracts of COS cells transiently transfected with carboxy- terminally (C) and amino-terminally (N) GFP- tagged TRIP8b-1a-5, TRIP8b-1a-4 or untagged Kv2.1 plasmid and probed with N291C/22 (left) and N212/7 (right) TC supe.
Adult rat brain immunohistochemistry.Adult rat brain membrane immunoblot.
Immunohistochemistry with antigen retrieval via citrate pretreatment.Adult rat hippocampus immunohistochemistry under standard protocol.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N277/7 (left) and N86/8 (right).
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N276A/15 (left) and N86/8 (right).
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N274/8 (left) and N86/8 (right).
Adult rat brain immunohistochemistry.Immunoblot versus crude membranes from adult rat brain (RBM) and WT and Clcn3 KO mouse brains probed with N258/5 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Fred Lamb (University of Iowa.
Immunoblot against bacterially expressed Thioredoxin (TRX) and/or 6xHistidine (His) tagged fusion proteins.
Adult rat brain membrane immunoblot.Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP- tagged TARPGamma2/3/4/8 or untagged Kv2.1 plasmid and probed with N245/36 (left) and N86/8 (right) TC supe.
Immunoblot versus low-speed pellet samples from wild-type (WT) and Stargazer mutant (STG) mice and probed with N245/1 TC supe. Data courtesy of Susumu Tomita, Yale University.
Adult rat brain membrane (RBM) immunoblot.
Adult rat brain membrane (RBM) immunoblot.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Tissue and transfected cell immunoblot: low speed pellet samples from adult rat brain (RBLSP) and extracts of COS cells transiently transfected with Myc-tagged PEX6 or untagged Kv2.1 plasmids and probed with N233/8 (left) and K89/34 (right).
Adult rat hippocampus (top), thalamus (bottom left) and superior colliculus (bottom right) immunohistochemistry with antigen retrieval via pepsin pretreatment.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged MMACHC or untagged Kv2.1 plasmid and probed with N230/21 (left) and N52A/42 (right) TC supe.
Adult rat brain immunohistochemistry.

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