Our CD55 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human CD55 (Extracellular region) and is Protein G purified. It is great for use in WB, ICC, IP.
Immunocytochemical labeling of CD55 in paraformaldehyde fixed human MDA-MB-231 breast cancer cells. The cells were labeled with mouse monoclonal anti-CD55 (CM0021). The antibody was detected using goat anti-mouse DyLight® 594.
CD55, also known as Decay-accelerating factor (DAF) is an inhibitor of the complement system, and is broadly expressed in malignant tumours. In cancer, CD55 has been implicated in tumorigenesis, neoangiogenesis, and metastasis. CD55 may decrease complement mediated tumor cell lysis, inhibit tumor apoptosis, and promote invasive cancer cell motility. These roles in cancer may involve binding to the seven-span transmembrane receptor CD97. In neuroblastoma cells, CD55 contributes to growth of colonies and to invasion of cells, but not to stemness. In neuroblastoma cells, CD55 is upregulated in a small population of cells that are HIF-2α positive. This CD55 positive subpopulation is highly invasive and has low adhesion to fibronectin and collagen. In addition, CD55 expression correlates with poor prognosis in neuroblastoma patients.
Protein G Purified
Monoclonal
M002
IgG2a
ICC, IP, WB
Mouse
CD55
60-80
Clone M002 was generated from proprietary antigen related to the extracellular region of human CD55 from the MDA-MB-231 breast cancer cell line.
Human
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:500
ICC: 1:100
IP: 1:50
Unconjugated
Clone M002 detects 60-80 kDa* bands corresponding to the molecular mass of CD55 on SDS-PAGE immunoblots of "native" A431, A549, and MDA-MB-231 cell lysates. The antibody also detects a "native" recombinant human CD55 extracellular region, but does not detect the denatured form of CD55. The antibody works for native western blot, immunoprecipitation, protein ELISA, and immunocytochemistry.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
