Our Anti-GABAA Receptor α4, rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GABAA Receptor α4, and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~64 kDa α4-subunit of the GABAA-R.
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunitsresults in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
IHC, WB
Rabbit
GABRA4
64 kDa
Fusion protein from the cytoplasmic loop of the α4 subunit of rat GABAA receptor.
Mouse, Rat
AB_2800407
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
IHC: 1:300
Unconjugated
Specific for endogenous levels of the ~ 64 kDa α4-subunit of the GABAA receptor.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
