Our Anti-LAMP1 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human LAMP1 and is Protein G purified. It is great for use in WB, IHC, ICC.
Immunolabeling of HeLa cells showing vesicular staining of LAMP1 protein (cat. 1030-LAMP1, red, 1:2000) accumulated in swollen lysosomes, while Anti-Vimentin (cat. 2105-VIM, green, 1:500) specifically labels the intermediate filament network in these cells. The blue stain is DAPI to reveal nuclear DNA.
Lysosomal Associated Membrane Protein1 (LAMP1) is a protein that is localized primarily in lysosomes but may also be present on late endosomes and the plasma membrane. LAMP1 antibodies are therefore widely used as lysosome markers. It has recently been suggested that lysosomes are activated in microglia in the progression of multiple system atrophy (MSA) and thus play a key role in its pathology (Makioka et al., 2012).
Protein G Purified
Monoclonal
5H6
IgG1
ICC, IHC, WB
Mouse
LAMP1
100 kDa
Recombinant full length human LAMP1 expressed in and purified from E. coli.
Human
AB_2492140
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Protein G purified culture supernatant
PBS + 10 mM NaN3.
WB: 1:5000
IHC: 1:2000
ICC: 1:500-1:2000
Unconjugated
Specific for endogenous levels of the ~100 kDa LAMP1 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
CD107 antigen like family member A antibody, CD107 antigen-like family member A antibody, CD107a antibody, CD107a antigen antibody, LAMP 1 antibody, LAMP-1 antibody, LAMP1 antibody, LAMP1_HUMAN antibody, LAMPA antibody, LGP120 antibody, lgpA antibody, Lysosomal membrane glycoprotein 120KD antibody, Lysosomal Associated Membrane Protein 1 antibody, Lysosome associated membrane glycoprotein 1 antibody, Lysosome-associated membrane glycoprotein 1 antibody, Lysosome-associated membrane protein 1 antibody, OTTHUMP00000040663 antibody
Rojas, T., et al. 2019. Inositol hexakisphosphate kinase 3 promotes focal adhesion turnover via interactions with dynein intermediate chain 2. Proceedings of the National Academy of Sciences, 116(8), pp.3278-3287.
Cuevas, E., et al. 2020. Tauroursodeoxycholic acid (TUDCA) is neuroprotective in a chronic mouse model of Parkinson’s disease. Nutritional Neuroscience, Dec. 21, pp.1-18.