Our Anti-Mnk2b rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Mnk2b and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of HeLa lysate showing specific immunolabeling of the ~47 kDa Mnk2b isoform in the first lane (-). Specificity is shown in the third lane (Mnk2b) where immunolabeling is blocked by preadsorption of the Mnk2b peptide used as the antigen, but not by the Mnk2a peptide in the second lane.
MAP kinase-interacting kinases Mnk1 and Mnk2, are activated by the MAP kinases, ERK and p38 (Waskiewicz et al., 1997). Additionally, Mnk1 and Mnk2 have been shown to phosphorylate the translation initiation factor eIF4E (Fukunaga and Hunter 1997; Waskiewicz et al., 1997). Alternative splicing of the MKNK2 gene results in two isoforms with different C-termini: one that has a MAPK-binding domain, Mnk2a, and one that does not, Mnk2b (Parra et al., 2005). Recent studies have indicated that Mnk2a has tumor suppressive activity while Mnk2b is pro-oncogenic (Maimon et al., 2014).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
MKNK2
47 kDa
Synthetic peptide from the C-terminal region, specific to the human Mnk2b isoform. The sequence of the antigen has no homology with Mnk1 or Mnk2a.
Human
Non-Human Primate
AB_2631043
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via chromatography on peptide immunogen column.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~47 kDa Mnk2b protein. Immunolabeling is blocked by preadsorption with the Mnk2b peptide used as antigen, but not by Mnk2a peptide.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Fukunaga, R. and Hunter, T., 1997. MNK1, a new MAP kinase‐activated protein kinase, isolated by a novel expression screening method for identifying protein kinase substrates. The EMBO journal, 16(8), pp.1921-1933. PMID: 9155018
Maimon, A., Mogilevsky, M., Shilo, A., Golan-Gerstl, R., Obiedat, A., Ben-Hur, V., Lebenthal-Loinger, I., Stein, I., Reich, R., Beenstock, J. and Zehorai, E., 2014. Mnk2 alternative splicing modulates the p38-MAPK pathway and impacts Ras-induced transformation. Cell Reports, 7(2), pp.501-513. PMID: 24726367
Parra, J.L., Buxadé, M. and Proud, C.G., 2005. Features of the catalytic domains and C termini of the MAPK signal-integrating kinases Mnk1 and Mnk2 determine their differing activities and regulatory properties. Journal of Biological Chemistry, 280(45), pp.37623-37633. PMID: 16162500
Waskiewicz, A.J., Flynn, A., Proud, C.G. and Cooper, J.A., 1997. Mitogen‐activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2. The EMBO journal, 16(8), pp.1909-1920. PMID: 9155017
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