Our Anti-Synapsin I rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Synapsin I and is antigen affinity purified from pooled serum. It is great for use in WB, ICC, IP.
Western blot of 10ug of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.
Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ICC, IHC, IP, WB
Rabbit
SYN1
78 kDa
Native protein purified from bovine brain.
Human, Mouse, Rat
AB_2492241
Reconstitute in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Lyophilized
Prepared from pooled rabbit serum by affinity purification using a column to which the native protein was coupled.
Specific for endogenous levels of the ~78 kDa synapsin I doublet. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody
Wahba, G., et al. 2016. A rapid and efficient method for dissociated cultures of mouse myenteric neurons. Journal of Neuroscience Methods, 261, pp.110-116.
Magupalli, V.G., et al. 2013. Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2. 1 calcium channels. Journal of Biological Chemistry, 288(7), pp.4637-4648.
Davies, K.D., et al. 2008. Long term synaptic depression that is associated with GluR1 dephosphorylation but not α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization. Journal of Biological Chemistry, 283(48), pp.33138-33146.
Grosshans, D.R., et al. 2002. LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nature neuroscience, Jan;5(1):27-33.
