Anti-Dicer Antibody (N167/7)

Name: Anti-Dicer Antibody
Brand: NeuroMab
SKU: 75-196
Price: 319.0 USD
Availability: InStock

Our Anti-Dicer mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N167/7. It is KO validated, detects human, mouse, and rat Dicer, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot versus adult rat brain membranes (RBM) and extracts of embryonic stem (ES) cells from wild-type (WT) and Dicer knockout (KO) mice probed with N167/7 (left) and N52A/42 (right, loading control) TC supe. Mouse ES cell samples courtesy of Chryssa Kanellopoulou and David Livingston, Harvard.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-196

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Dicer

Target Description

Endoribonuclease Dicer or Dicer 1, Ribonuclease III is encoded by the gene DICER1. DICER1 acts as a double stranded (ds) ribonuclease and plays a role in short post transcriptional gene silencing. DICER1 cleaves full length dsRNA and miRNA into smaller moleclues 21-23 nucleotides in length, called siRNAs and microRNAs. Dicer1 is expressed in a variety of tissues, including brain. Diseases associated with this gene include Pleuropulmonary Blastoma, Glow Syndrome and Wilms Tumor.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N167/7

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Dicer1 Dicer Mdcr

Molecular Weight

215 kDa

Antigen

Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer (accession number Q8R418) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2093085

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.124

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Endoribonuclease Dicer (EC 3.1.26.3) (Double-strand-specific ribonuclease mDCR-1)

UniProt Details

UniProt (Human): Q9UPY3
UniProt (Immunogen Species): Q8R418

Product Specific References for Applications and Species

Western Blot: Mouse
Additional Publications: Unspecified

Western Blot: Mouse
PMID	Dilution	Publication
25771167	1:500	Hsu, P.K., et al. 2015. The BDNF Val66Met variant affects gene expression through miR-146b.. Neurobiology of Disease, 228-237.
22385959	not listed	Huang, Y.W.A., et al. 2012. Dual regulation of miRNA biogenesis generates target specificity in neurotrophin-induced protein synthesis. Cell, 933-946.

Additional Publications: Unspecified
PMID	Publication
25421653	Satterlee, J.S., et al. 2015. Community resources and technologies developed through the NIH Roadmap Epigenomics Program.. Methods in Molecular Biology, 27-49.

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Anti-Dicer Antibody (N167/7)

SKU: 75-196

Product Details

Product Specific References for Applications and Species

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