Our Anti-Histone H3-acetyl-Lys56 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N307/12. It detects human, mouse, and rat Histone H3-acetyl-Lys56, and is purified by Protein A chromatography. It is great for use in CI, DB.
"Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N307/12 TC supe (left) and a rabbit polyclonal control (right). ."
This antibody recognizes a modified Histone H3.1 (which is also known by many other names and is recognized by the accession number P68431 and is encoded by the gene H3C1 (which is also known by many other names). Histones organize DNA into nucleosomes and are the main structural component of chromatin. Reacts preferentially with Histone H3 acetylatedLys56 than with unacetylated-Lys56 or with other H3 acetylated-Lys epitopes (Lys4, Lys9, Lys14, Lys18, Lys23, Lys27, Lys36 and Lys64).
Synthetic peptide amino acids 51-60 (EIRRYQ[acetyl-K]STE) of human Histone H3.1 (accession number P68431).
Human, Mouse, Rat
AB_2315850
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Unconjugated
Reacts preferentially with Histone H3 acetylated-Lys56 than with unacetylated-Lys56 or with other H3 acetylated-Lys epitopes (Lys4, Lys9, Lys14, Lys18, Lys23, Lys27, Lys36 and Lys64)
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Satterlee, J.S., et al. 2015. Community resources and technologies developed through the NIH Roadmap Epigenomics Program. Methods in Molecular Biology, 27-49.
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