Anti-Synaptotagmin-3 Antibody (N278/19)

Name: Anti-Synaptotagmin-3 Antibody
Brand: NeuroMab
SKU: 75-269
Price: 319.0 USD
Availability: InStock

1 Citation

Our Anti-Synaptotagmin-3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N278/19. It detects human, mouse, and rat Synaptotagmin-3, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP- tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N278/19 (left) and N86/8 (right).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-269

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Synaptotagmin-3

Target Description

Synaptotagmins are a family of calcium sensing, membrane trafficking proteins. 17 different synaptotagmins have been identified in mammals. Synaptotagmin 3 is predominantly expressed in postsynaptic endocytic zones of neurons and has recently been shown to internalize AMPA receptors to depress synaptic strength and promote forgetting (Awasthi A. et al., 2019).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N278/19

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Syt3

Molecular Weight

75 kDa

Antigen

Fusion protein amino acids 442-533 (cytoplasmic C2B domain) of mouse Synaptotagmin-3 (accession number O35681) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_11001556

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with other Synaptotagmins

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Synaptotagmin-3 (Synaptotagmin III) (SytIII)

UniProt Details

UniProt (Human): Q9BQG1
UniProt (Immunogen Species): O35681

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blot: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
30545844	not listed	Awasthi, A., et al. 2019. Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting. Science, .

Western Blot: Mouse
PMID	Dilution	Publication
30545844	not listed	Awasthi, A., et al. 2019. Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting. Science, .

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Anti-Synaptotagmin-3 Antibody (N278/19)

SKU: 75-269

Product Details

Product Specific References for Applications and Species

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