Anti-KCNU1/Slo3 pH-Sensitive Maxi-K+ Channel Antibody (N2/16)
Our Anti-KCNU1/Slo3 pH-sensitive maxi-K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N2/16. It is KO validated, detects mouse, Xenopus KCNU1/Slo3 pH-sensitive maxi-K+ channel, and is purified by Protein A chromatography. It is great for use in EM, ICC, IP, WB.
Immunoblot versus (E) membrane proteins from wild- type (wt), Slo3-eGFP and Slo3 knockout (Slo3-/-) mouse testes and (F) immunoprecipitation products from total testes proteins pulled down with N2/16 probed with N2/16. Data courtesy of Christopher Lingle (Washington University) and reproduced with permission from the Proceedings of the National Academy of Sciences of the United States of America (2011 Zeng et al., PMID 21427226).
Roll over image to zoom in
Click on image to zoom
Gerbil, Mouse
EM, ICC, IP, WB
Mouse
KO Validated
Product Specific References for Applications and Species
| Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 22084117 | not listed | Yang, C., et al. 2011. LRRC52 (leucine-rich-repeat-containing protein 52), a testis-specific auxiliary subunit of the alkalization-activated Slo3 channel.. PNAS: USA, 19419-19424. |
| 21427226 | 5ug | Zeng, X.H., et al. 2011. Deletion of the Slo3 gene abolishes alkalization-activated K+ current in mouse spermatozoa.. PNAS: USA, 5879-5884. |
| Immunoprecipitation: Xenopus | ||
| PMID | Dilution | Publication |
| 22084117 | not listed | Yang, C., et al. 2011. LRRC52 (leucine-rich-repeat-containing protein 52), a testis-specific auxiliary subunit of the alkalization-activated Slo3 channel.. PNAS: USA, 19419-19424. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 31270758 | 1:1000 | Chávez, J.C., et al. 2019. A cytoplasmic Slo3 isoform is expressed in somatic tissues. Molecular Biology Reports, 5561-5567. |
| 25675513 | not listed | Zeng, X.H., et al. 2015. SLO3 auxiliary subunit LRRC52 controls gating of sperm KSPER currents and is critical for normal fertility.. PNAS: USA, 2599-2604. |
| 20138882 | 2.8ug/ml | Santi, C.M., et al. 2010. The SLO3 sperm-specific potassium channel plays a vital role in male fertility.. FEBS Letters, 1041-1046. |
| 19516020 | not listed | Wang, H., et al. 2009. A novel, single, transmembrane protein CATSPERG is associated with CATSPER1 channel protein.. Biology of Reproduction, 539-544. |
| Western Blot: Xenopus | ||
| PMID | Dilution | Publication |
| 19578543 | 1:200 | Yang, C.T., et al. 2009. Interactions between beta subunits of the KCNMB family and Slo3: beta4 selectively modulates Slo3 expression and function.. PLoS One, e6135. |
| 19338774 | 1:10 (supe) | Martínez-López, P., et al. 2009. Mouse sperm K+ currents stimulated by pH and cAMP possibly coded by Slo3 channels.. Biochemical and Biophysical Research Communications, 204-209. |