Anti-TRIP8b (Exon 1b) Antibody (N212A/34)

4 Citations

Our Anti-TRIP8b (exon 1b) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N212A/34. It is KO validated, detects human, mouse, and rat TRIP8b (exon 1b), and is purified by Protein A chromatography. It is great for use in ICC, WB.

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212A/34 (left) or N86/8 (right) TC supe.
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212A/34 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).
Immunoblot against crude membranes from whole rat (RBM) and mouse (MBM) brain and from human cerebellum (HBM(Cb)], cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with N212A/34 (left) or N52A/42 (right) TC supe.
Western blotting results showing TRIP8b (1a-4), and TRIP8b(1b-2) labeling in hippocampal CA1 region of control group rats either treated with saline or a PKA inhibitor(H89) and pentylenetetrazole (PTZ) treated rats under the same experimental conditions. Image from publication CC-BY-4.0. PMID: 36018183
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212A/34 (left) or N86/8 (right) TC supe.
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Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212A/34 (left) or N86/8 (right) TC supe.
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212A/34 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).
Immunoblot against crude membranes from whole rat (RBM) and mouse (MBM) brain and from human cerebellum (HBM(Cb)], cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with N212A/34 (left) or N52A/42 (right) TC supe.
Western blotting results showing TRIP8b (1a-4), and TRIP8b(1b-2) labeling in hippocampal CA1 region of control group rats either treated with saline or a PKA inhibitor(H89) and pentylenetetrazole (PTZ) treated rats under the same experimental conditions. Image from publication CC-BY-4.0. PMID: 36018183

Human, Mouse, Rat
ICC, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet
KO Validated KO Validated

SKU: 75-245

Volume: 100 µL
1-2 business days
Price:
$329.00

Product Specific References for Applications and Species

Western Blot: Mouse
PMID Dilution Publication
29662202not listedYun, S., et al. 2018. Stimulation of entorhinal cortex-dentate gyrus circuitry is antidepressive. Nature Medicine, 658-666.
Western Blot: Rat
PMID Dilution Publication
360181831:200Luo, X.D., et al. 2023. Activation of Metabotropic Glutamate Receptor 1 Regulates Hippocampal CA1 Region Excitability in Rats with Status Epilepticus by Suppressing the HCN1 Channel. Neural Regeneration Research, 594-602.
253011011:200Zhou, M., et al. 2015. Imbalance of HCN1 and HCN2 expression in hippocampal CA1 area impairs spatial learning and memory in rats with chronic morphine exposure.. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 207-214.
248386251:200Li, C.J., et al. 2014. Activation of GABAB receptors ameliorates cognitive impairment via restoring the balance of HCN1/HCN2 surface expression in the hippocampal CA1 area in rats with chronic cerebral hypoperfusion.. Molecular Neurobiology, 704-720.

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